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  • Organism:e. coli (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Authority Record Last Update Mentions Count
pSLQ1651-sgRNA(F+E)-sgGal4
 
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1+ mentions
RRID:Addgene_100549 sgGal4 E. coli Ampicillin PMID:28841410 Backbone Marker:Addgene #51024; Vector Backbone:pSLQ1651-sgRNA(F+E); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:18:18 8
relA+_NS1
 
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RRID:Addgene_100916 RelA fragment from E.coli E. coli Spectinomycin and Streptomycin PMID:29241543 Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin Addgene 2026-09-26 02:18:21 0
Ptrc::galP_NS1
 
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1+ mentions
RRID:Addgene_101843 Ptrc promoter driving GalP transporter expression E. coli Nourseothricin (clonNat) PMID:28430105 Backbone Size:5300; Vector Backbone:pAM1303; Vector Types:Cloning vector; Bacterial Resistance:Nourseothricin (clonNat) Addgene 2026-09-26 02:18:26 1
pTR47m4-GFP
 
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RRID:Addgene_102436 Formaldehyde repressor protein (frmR) E. coli Ampicillin PMID:28921510 Vector Backbone:pTR47m4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:18:28 0
pKSJ331
 
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1+ mentions
RRID:Addgene_103063 Colicin E1 and E1 immunity protein E. coli Ampicillin PMID:19029376 Insert was cloned from ~base pair 5015 to ~500 of the circular ColE1 plasmid, where numbering begins within the colicin E1 gene. The ColE1 sequences include both the promoter for colicin E1 and for E1 immunity protein, which is transcribed in the opposite direction from the colicin gene. The cloned DNA does not include the intact kil (lysis protein) gene. Backbone Marker:NEB; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:18:32 1
pKSJ376
 
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RRID:Addgene_103065 colicin E1 immunity protein E. coli Ampicillin PMID:27795317 The genes for colicin E1 with the R domain deletion and E1 immunity protein are transcribed from opposite strands of the vector. Colicin synthesis is under control of the vector inducible promoter; immunity protein synthesis is controlled by its own promoter. Backbone Marker:Novagen EMD/Millipore; Backbone Size:5627; Vector Backbone:pET52-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:18:32 0
PVS10
 
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RRID:Addgene_104398 rpoA-rpoB-rpoC-6xHis and rpoZ E. coli Ampicillin PMID:25665556 Plasmid pVS10 encodes the E. coli rpoA-rpoB-rpoC [His6] and rpoZ ORFs under control of T7 promoter. Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:18:44 2
PIA900
 
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1+ mentions
RRID:Addgene_104401 rpoA, B, C, and Z E. coli Ampicillin PMID:25665556 pIA900 (rpoA, B, C, and Z) encodes a C-terminally His-tagged β′ which can be removed using TEV protease. Linker with TEV-His10 cloned between NheI and StuI sites of pIA787; NheI site is gone Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression, E. coli expression vector; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:18:44 3
pET28b – E. coli RecE residues 564-868
 
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RRID:Addgene_104534 RecE residues 564-866 (C-terminal nuclease domain) E. coli Kanamycin PMID:19446525 Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Addgene 2026-09-26 02:18:45 0
pBad-HPII
 
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RRID:Addgene_105839 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:18:55 0
pBad-HPII TAG348
 
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RRID:Addgene_105844 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin TAG stop codon at site 348 Addgene 2026-09-26 02:18:55 0
pBad-HPII TAG568
 
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RRID:Addgene_105845 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin TAG stop codon at site 568 Addgene 2026-09-26 02:18:55 0
pBad-HPII TAG283
 
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RRID:Addgene_105843 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin TAG stop codon at site 283 Addgene 2026-09-26 02:18:55 0
pIBA2-SpyTag-MBP
 
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RRID:Addgene_107422 Maltose-binding protein (MalE) E. coli Ampicillin PMID:30600549 Spycatcher was published in Zakeri et al. (2012), PNAS 109, ppE690-7 Backbone Marker:IBA GmbH; Backbone Size:3286; Vector Backbone:pASK-IBA2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:19:07 0
pATLIC
 
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RRID:Addgene_110287 yfaL E. coli Ampicillin PMID:22344647 Addgene QC finds S92G, G99S, and K330R mutations in yfaL as well as a stop codon inserted before the His tag. The depositor notes these are not of functional concern Backbone Size:3986; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:19:32 0
pOmpW
 
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RRID:Addgene_112121 OmpW E. coli Ampicillin PMID:18641391 Backbone Size:5600; Vector Backbone:pET11A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin N-terminal signal sequence not included and replaced with Methionine. Addgene 2026-09-26 02:19:49 0
pOmpT
 
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RRID:Addgene_112123 OmpT E. coli Ampicillin PMID:18641391 Backbone Size:5636; Vector Backbone:pET11A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin N-terminal signal sequence not included and replaced with Methionine. Addgene 2026-09-26 02:19:49 0
pET-2(KW)2-mCherry
 
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RRID:Addgene_112738 2(KW)2-mCherry E. coli Ampicillin PMID:26264666 Note: The mCherry insert contains a N9Q mutation. The depositor has confirmed this does not affect plasmid function. Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:19:55 0
pET-2HMG-eGFP
 
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RRID:Addgene_112737 2HMG-eGFP E. coli Ampicillin Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:19:55 0
pPB-R1R2-EM7neoPheS
 
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1+ mentions
RRID:Addgene_112923 neoPheS E. coli Ampicillin PMID:30477585 Backbone Size:3470; Vector Backbone:pPB_LR5; Vector Types:Mammalian Expression, piggyBac transposon Gateway destination vector; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:19:57 1

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