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  • Organism:caenorhabditis elegans (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
Punc-4::NGL-1::spGFP1-10
 
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1+ mentions
RRID:Addgene_65827 NLG-1 Caenorhabditis elegans Ampicillin PMID:18255029 Backbone Marker:C.Bargmann; Backbone Size:3500; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:08:54 1
dg263_pENTR_ChrimsonCel
 
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RRID:Addgene_66101 Chrimson Caenorhabditis elegans Kanamycin PMID:26022242 This construct was produced through de novo gene synthesis. Chrimson amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346. Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-29 01:08:57 1
dg264_pENTR_ChronosCel
 
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RRID:Addgene_66102 Chronos Caenorhabditis elegans Kanamycin PMID:26022242 This construct was produced through de novo gene synthesis. Chronos amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346. Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-29 01:09:01 0
pClneoEGFPC-let60 K16N
 
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RRID:Addgene_66861 Let-60 Caenorhabditis elegans Ampicillin PMID:23103556 Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Lysine 16 is mutated to asparagine 2026-08-29 01:09:03 0
pDD285
 
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RRID:Addgene_66826 mKate2-C1^SEC^3xFlag Caenorhabditis elegans Ampicillin PMID:26044593 THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD287 (https://www.addgene.org/70685/). The full sequence for this plasmid can still be found by clicking the link next to "Depositor Sequences". IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined. Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:09:03 0
pDD284
 
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RRID:Addgene_66825 TagRFP-T-C1^SEC^3xFlag Caenorhabditis elegans Ampicillin PMID:26044593 THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD286 (https://www.addgene.org/70684/). The full sequence for this plasmid can still be found by clicking the link next to "Addgene Sequences" and scrolling to the bottom of the page for "Full Addgene NGS sequence". IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined. Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:09:05 5
pClneoMyc' CST-1
 
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RRID:Addgene_66859 CST-1 Caenorhabditis elegans Ampicillin PMID:23103556 Addgene sequencing results find amino acid changes S48N and H50R compared to available CST-1 reference sequences. Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S48N, H50R, S257P and V340A mutations in CST-1 compared to GenBank reference sequence NP_001024575.2. CST-1 ends at amino acid 444 2026-08-29 01:09:05 1
pPD95.75-cYAP-1 S104A
 
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RRID:Addgene_66858 YAP-1 Caenorhabditis elegans Ampicillin PMID:23396260 I performed PCR on C.elegans genome to isolate the genome of YAP-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon. Serine 104 was mutated to alanine. Backbone Marker:Addgene; Vector Backbone:PD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin Serine104 is mutated to alanine 2026-08-29 01:09:03 0
pClneoMyc'-cYAP-1
 
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RRID:Addgene_66854 YAP-1 Caenorhabditis elegans Ampicillin PMID:23396260 Addgene sequencing results find amino acid changes Q48R and Y213F compared to available YAP-1 reference sequences. Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:09:03 0
pPD95.75 RSF-1
 
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RRID:Addgene_66863 RSF-1 Caenorhabditis elegans Ampicillin PMID:23103556 I performed PCR on C.elegans genome to isolate the genome of RSF-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon. Backbone Marker:Addgene; Vector Backbone:PD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:09:04 0
pJJR82
 
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1+ mentions
RRID:Addgene_75027 EGFP^SEC^3xFlag Caenorhabditis elegans Ampicillin PMID:32586975 Backbone Size:3854; Vector Backbone:pDD282; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:10:17 6
pJJR81
 
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1+ mentions
RRID:Addgene_75029 tagBFP2^SEC^3xFlag Caenorhabditis elegans Ampicillin Backbone Size:3854; Vector Backbone:pDD282; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:10:18 5
pJJR83
 
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1+ mentions
RRID:Addgene_75028 mCherry^SEC^3xFlag Caenorhabditis elegans Ampicillin PMID:32586975 Backbone Size:3854; Vector Backbone:pDD282; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:10:17 8
pSR02
 
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1+ mentions
RRID:Addgene_69149 mCherry Caenorhabditis elegans Ampicillin PMID:26144318 Backbone Marker:Erik Jorgensen; Backbone Size:7606; Vector Backbone:pCFJ356; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin codon-optimzed index 1.0 2026-08-29 01:09:22 1
pSR49
 
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RRID:Addgene_69259 TagBFP2 Caenorhabditis elegans Ampicillin PMID:26144318 Backbone Marker:Andrew Fire; Backbone Size:3417; Vector Backbone:pPD49_26; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin codon-optimzed index 1.0, TagBFP2 contains 2 single nucleotide changes compared to TagBFP 2026-08-29 01:09:23 0
pSR40
 
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RRID:Addgene_69257 Cre recombinase Caenorhabditis elegans Ampicillin PMID:26144318 Backbone Marker:Erik Jorgensen; Backbone Size:8941; Vector Backbone:pCFJ355; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin codon-optimzed index 1.0 2026-08-29 01:09:23 0
pUA97
 
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RRID:Addgene_69340 ceBFP SEC 3xFlag Caenorhabditis elegans Ampicillin The plasmid was made based on pDD268 from Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.178335. Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin sqt-1(e1350) 2026-08-29 01:09:24 0
pJW4.33
 
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RRID:Addgene_62880 GST-MEG-3 Caenorhabditis elegans Ampicillin PMID:25535836 Backbone Size:4984; Vector Backbone:pGEX6p1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:08:31 0
mg409
 
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RRID:Addgene_73368 TSMod::unc-70 Caenorhabditis elegans Kanamycin PMID:24561618 Vector Backbone:pCR; Vector Types:; Bacterial Resistance:Kanamycin 2026-08-29 01:10:02 0
JH04
 
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RRID:Addgene_73513 pmex-5::mRuby2::PH::tbb-2 Caenorhabditis elegans Ampicillin PMID:27385332 Vector Backbone:modified pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-29 01:10:03 0

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