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Species: Caenorhabditis elegans
Genetic Insert: Let-60
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23103556
Proper citation: RRID:Addgene_66861 Copy
Species: Caenorhabditis elegans
Genetic Insert: mKate2-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044593
Comments: THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD287 (https://www.addgene.org/70685/). The full sequence for this plasmid can still be found by clicking the link next to "Depositor Sequences".
IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined.
Proper citation: RRID:Addgene_66826 Copy
Species: Caenorhabditis elegans
Genetic Insert: TagRFP-T-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044593
Comments: THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD286 (https://www.addgene.org/70684/). The full sequence for this plasmid can still be found by clicking the link next to "Addgene Sequences" and scrolling to the bottom of the page for "Full Addgene NGS sequence".
IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined.
Proper citation: RRID:Addgene_66825 Copy
Species: Caenorhabditis elegans
Genetic Insert: CST-1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23103556
Comments: Addgene sequencing results find amino acid changes S48N and H50R compared to available CST-1 reference sequences.
Proper citation: RRID:Addgene_66859 Copy
Species: Caenorhabditis elegans
Genetic Insert: YAP-1
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:PD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396260
Comments: I performed PCR on C.elegans genome to isolate the genome of YAP-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon. Serine 104 was mutated to alanine.
Proper citation: RRID:Addgene_66858 Copy
Species: Caenorhabditis elegans
Genetic Insert: YAP-1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396260
Comments: Addgene sequencing results find amino acid changes Q48R and Y213F compared to available YAP-1 reference sequences.
Proper citation: RRID:Addgene_66854 Copy
Species: Caenorhabditis elegans
Genetic Insert: RSF-1
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:PD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23103556
Comments: I performed PCR on C.elegans genome to isolate the genome of RSF-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon.
Proper citation: RRID:Addgene_66863 Copy
Species: Caenorhabditis elegans
Genetic Insert: rab-3::NLS::GCaMP6s
Vector Backbone Description: Vector Backbone:Not Available; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26712014
Proper citation: RRID:Addgene_68119 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-16
Vector Backbone Description: Backbone Marker:Andrew Fire (Addgene Plasmid 1497); Backbone Size:4406; Vector Backbone:pPD95.81; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23805378
Comments: The D->V point mutation in DAF-16 has no functional consequence.
Proper citation: RRID:Addgene_52032 Copy
Species: Caenorhabditis elegans
Genetic Insert: fli-1
Vector Backbone Description: Backbone Marker:Fire lab; Backbone Size:4487; Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18485202
Proper citation: RRID:Addgene_52348 Copy
Species: Caenorhabditis elegans
Genetic Insert: swan-1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6299; Vector Backbone:pCMV-3xFLAG; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16980389
Proper citation: RRID:Addgene_52349 Copy
Species: Caenorhabditis elegans
Genetic Insert: rack-1
Vector Backbone Description: Backbone Marker:Fire lab; Backbone Size:4487; Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21124943
Proper citation: RRID:Addgene_52351 Copy
Species: Caenorhabditis elegans
Genetic Insert: Dre-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5404; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24613396
Proper citation: RRID:Addgene_52517 Copy
Species: Caenorhabditis elegans
Genetic Insert: Lin-29 b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5442; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24613396
Proper citation: RRID:Addgene_52515 Copy
Species: Caenorhabditis elegans
Genetic Insert: Blmp-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5462; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24613396
Proper citation: RRID:Addgene_52512 Copy
Species: Caenorhabditis elegans
Genetic Insert: TSMod::unc-70
Vector Backbone Description: Vector Backbone:pCR; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24561618
Proper citation: RRID:Addgene_73368 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5::mRuby2::PH::tbb-2
Vector Backbone Description: Vector Backbone:modified pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27385332
Proper citation: RRID:Addgene_73513 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5::GFPo::PH::tbb-2
Vector Backbone Description: Vector Backbone:modified pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27385332
Proper citation: RRID:Addgene_73510 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5::mPYet::PH::tbb-2
Vector Backbone Description: Vector Backbone:modified pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27385332
Proper citation: RRID:Addgene_73516 Copy
Species: Caenorhabditis elegans
Genetic Insert: SL2 + SV40 NLS (gdp-2/gdp-3 intergenic region)
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26837755
Proper citation: RRID:Addgene_73727 Copy
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