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Vector Backbone Description: Backbone Marker:Cambia; Vector Backbone:pCAMBIA1300; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:26644504
Comments: See genbank file for best annotation of features.
Proper citation: RRID:Addgene_71275 Copy
Vector Backbone Description: Backbone Marker:Thermo; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:30379827
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/14/277350 for BioRxiv preprint
Proper citation: RRID:Addgene_71534 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9000; Vector Backbone:pBI101; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:21343429
Comments: Aoyama, T. and Chua, N.-H. (1997) A glucocorticoid-mediated
transcriptional induction system in transgenic plants. Plant J.
11, 605–612
Proper citation: RRID:Addgene_71745 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:27084942
Comments: Please note that parental strain (MC4100) is streptomycin-resistant.
Proper citation: RRID:Addgene_69771 Copy
Vector Backbone Description: Backbone Size:7450; Vector Backbone:pDEST1K18ms; Vector Types:sucrose counter-selection allelic exchange vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:34032522
Proper citation: RRID:Addgene_72646 Copy
Vector Backbone Description: Backbone Size:2051; Vector Backbone:pENTR1A; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme. It serves to create intermediate supermodules with plant resistance cassettes.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 358-368bp
BsaI site #2 = 1796-1806bp
Proper citation: RRID:Addgene_48865 Copy
Vector Backbone Description: Backbone Marker:Messing Lab, PMID 1905257; Backbone Size:3772; Vector Backbone:pOK1/2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24146852
Proper citation: RRID:Addgene_48942 Copy
Species: Mus musculus
Genetic Insert: Olig2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pLP-IRES2-EGFP; Vector Types:Mammalian Expression, Cre/Lox, acceptor vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: mOlig2 was transferred from the donor vector (pDNR-mOlig2, Addgene plasmid #49397) to the acceptor vector using Cre-mediated recombination at the LoxP sites.
Proper citation: RRID:Addgene_49399 Copy
Species: Homo sapiens
Genetic Insert: Sox2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pLP-IRES2-EGFP; Vector Types:Mammalian Expression, Cre/Lox, acceptor vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: hSox2 was transferred from the donor vector (pDNR-hSox2, Addgene plasmid #49389) to the acceptor vector using Cre-mediated recombination at the LoxP sites.
Proper citation: RRID:Addgene_49391 Copy
Species: Discosoma sp.
Genetic Insert: monomeric red fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61649 Copy
Species: Auquorea victoria
Genetic Insert: enhanced yellow fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61655 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19843313
Proper citation: RRID:Addgene_61703 Copy
Vector Backbone Description: Backbone Size:11775; Vector Backbone:pEarleyGate-202; Vector Types:Plant Expression, BiFC assay; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19843313
Proper citation: RRID:Addgene_61705 Copy
Species: Pseudomonas putida strain CBB5
Genetic Insert: ndmA
Vector Backbone Description: Backbone Marker:igem parts registry; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23654268
Comments: The sequence of this plasmid is updated from the GenBank entry for pDCAF3 (KC619530.1) to include an IS5 insertion that occurred in the promoter region of the caffeine demethylation genes during passaging of the strain and a base change in the sequence of the gst9 gene. This plasmid still functions as described in the publication for pDCAF3.
Vector constitutively expresses N-demethylase genes under the control of the BBa_J23100 promoter (http://parts.igem.org/Part:BBa_J23100).
N-demethylases are only functional when grown at 30°C and require iron supplementation in minimal media. See:
Summers, R. M., Louie, T. M., Yu, C.-L., Gakhar, L., Louie, K.C., and Subramanian, M. (2012) Novel, highly specific Ndemethylases enable bacteria to live on caffeine and related purine alkaloids. J. Bacteriol. 194, 2041−2049.
For the quantification of caffeine (or other methylxanthine) content it is recommended to grow cells in M9 minimal medium supplemented with 2 g/L glucose and 2 g/L casein (M9GC).
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_65220 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:34717688
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167673 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:34717688
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167672 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:34717688
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167671 Copy
Vector Backbone Description: Backbone Size:13430; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32976518
Comments: Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants
https://academic.oup.com/plphys/article/146/2/323/6107098
Please note there are two mutations in HygR- E180D and A95S. Depositor confirms these do not affect plasmid function.
Proper citation: RRID:Addgene_174050 Copy
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161893 Copy
Species: Synthetic
Genetic Insert: pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2
Vector Backbone Description: Backbone Marker:Christopher Voigt's Lab; Vector Backbone:pTHSSd_1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:33850130
Proper citation: RRID:Addgene_161937 Copy
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