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Species: Other
Genetic Insert: Level 2 RECKLEEN plasmid
Vector Backbone Description: Vector Backbone:Custom built using Marburg Collection; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:41168338
Comments: Please visit https://doi.org/10.21203/rs.3.rs-5859838/v1 for preprint.
Proper citation: RRID:Addgene_233457 Copy
Species: Other
Genetic Insert: Level 2 RECKLEEN plasmid, Ptac lambda Red operon, Ptet SpG cas9, J23117 acrIIA4
Vector Backbone Description: Vector Backbone:Custom built using Marburg Collection; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:41168338
Comments: Please visit https://doi.org/10.21203/rs.3.rs-5859838/v1 for preprint.
Proper citation: RRID:Addgene_233458 Copy
Vector Backbone Description: Vector Backbone:pYH7; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:40195982
Comments: Please visit https://doi.org/10.21203/rs.3.rs-6229288/v1 for the article preprint.
Proper citation: RRID:Addgene_240097 Copy
Species: Aequorea victoria
Genetic Insert: superfolder green fluorescent protein (sfGFP)
Vector Backbone Description: Vector Backbone:pSEVAb86; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:40471122
Proper citation: RRID:Addgene_217503 Copy
Species: Aequorea victoria
Genetic Insert: superfolder green fluorescent protein (sfGFP)
Vector Backbone Description: Vector Backbone:pSEVAb84; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:40471122
Proper citation: RRID:Addgene_217502 Copy
Vector Backbone Description: Backbone Size:6440; Vector Backbone:pUC19; Vector Types:Expression vector for targeted integration; Bacterial Resistance:Apramycin
Defining Citation: PMID:34890250
Comments: For cloning in homology arms, can use Gateway, restriction ligation, or homology-based cloning such as Gibson/Infusion cloning. eGFP can be swapped for your gene of interest or can be fused (recommend homology base cloning).
Original pSelAct backbone was developed here:
Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151
pSelAct-Express was develop and tested here:
Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345
Proper citation: RRID:Addgene_174375 Copy
Species: Stackebrandtia nassauensis
Genetic Insert: inactivated SnaE
Vector Backbone Description: Vector Backbone:pOSV801; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:38947204
Comments: also encodes SnaA, SnaB, SnaC, SnaD, SnaO, SnaT1, SnaT2
Proper citation: RRID:Addgene_225057 Copy
Species: Stackebrandtia nassauensis
Genetic Insert: inactivated SnaA
Vector Backbone Description: Vector Backbone:pOSV801; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:38947204
Comments: also encodes SnaB, SnaC, SnaD, SnaE, SnaO, SnaT1, SnaT2
Proper citation: RRID:Addgene_225056 Copy
Species: Synthetic
Genetic Insert: AmR
Vector Backbone Description: Backbone Size:6370; Vector Backbone:pFNC-6; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39778677
Proper citation: RRID:Addgene_227670 Copy
Species: Stackebrandtia nassauensis
Genetic Insert: SnaA
Vector Backbone Description: Vector Backbone:pOSV801; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:38947204
Comments: encodes biosynthetic genes for threopeptin, including SnaA, SnaB, SnaC, SnaD, SnaE, SnaO, SnaT1, SnaT2
Proper citation: RRID:Addgene_225055 Copy
Genetic Insert: apramycin resistance gene
Vector Backbone Description: Vector Backbone:pSGAb; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Proper citation: RRID:Addgene_226171 Copy
Vector Backbone Description: Backbone Size:9558; Vector Backbone:pBAC2015, pGM1190, and pUC19; Vector Types:; Bacterial Resistance:Apramycin
Defining Citation: PMID:39666857
Proper citation: RRID:Addgene_227579 Copy
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:39666857
Proper citation: RRID:Addgene_227581 Copy
Species: Synthetic
Genetic Insert: Codon optimized APOBEC1-nCas9-UGI fusion protein
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:38340355
Proper citation: RRID:Addgene_209447 Copy
Species: Synthetic
Genetic Insert: Codon optimized APOBEC1-nCas9-UGI fusion protein
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:38340355
Proper citation: RRID:Addgene_209446 Copy
Species: Synthetic
Genetic Insert: T7RNAP Circuit
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:35366417
Proper citation: RRID:Addgene_199993 Copy
Vector Backbone Description: Vector Backbone:pMMB207; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39162403
Comments: Gebhardt lab ID # pMJG588.
Proper citation: RRID:Addgene_218359 Copy
Vector Backbone Description: Vector Backbone:pMMB207; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39162403
Comments: Gebhardt lab ID # pMJG120.
Proper citation: RRID:Addgene_218357 Copy
Species: S. coelicolor (bacteria)
Genetic Insert: scbe3-NG-HF1
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin
Defining Citation: PMID:38971862
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218160 Copy
Species: S. coelicolor (bacteria)
Genetic Insert: escbe3-NG
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin
Defining Citation: PMID:38971862
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218163 Copy
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