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Species: Mesoplasma florum
Genetic Insert: oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101316 Copy
Species: Mesoplasma florum
Genetic Insert: oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101310 Copy
Species: Mesoplasma florum
Genetic Insert: oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101311 Copy
Vector Backbone Description: Vector Backbone:pCAP05; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28874475
Comments: pCAP05 can be linearized through the restriction sites ClaI and XhoI. Linear vector can be used for the construction of cluster-specific capture vectors using the same methodology as described for pCAP03.
Proper citation: RRID:Addgene_102920 Copy
Vector Backbone Description: Backbone Size:2026; Vector Backbone:pRH144; Vector Types:mini-transposon system suitable for sequencing, shuttling and gene fusions; Bacterial Resistance:Tetracycline
Defining Citation: PMID:8566811
Comments: Artificial DNA for plasmid pMin1
GenBank: Z50148.1
Proper citation: RRID:Addgene_102959 Copy
Vector Backbone Description: Backbone Marker:Erb Lab; Backbone Size:6558; Vector Backbone:pTE100; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25105793
Proper citation: RRID:Addgene_59399 Copy
Vector Backbone Description: Backbone Marker:Erb Lab; Backbone Size:6558; Vector Backbone:pTE100; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25105793
Proper citation: RRID:Addgene_59398 Copy
Genetic Insert: rfp
Vector Backbone Description: Backbone Size:7569; Vector Backbone:pCM62; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24219429
Proper citation: RRID:Addgene_59775 Copy
Species: Xanthamonas
Genetic Insert: NS3
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3007; Vector Backbone:pTC14; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Comments: Note: Restriction digests show that this pNS series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_50611 Copy
Species: Xanthamonas
Genetic Insert: NS9
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3007; Vector Backbone:pTC14; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Comments: Note: Restriction digests show that this pNS series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_50617 Copy
Species: Escherichia coli
Genetic Insert: KanR
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23656183
Proper citation: RRID:Addgene_55750 Copy
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7535; Vector Backbone:pQF; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23995928
Proper citation: RRID:Addgene_48100 Copy
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7535; Vector Backbone:pQF; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23995928
Proper citation: RRID:Addgene_48097 Copy
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7535; Vector Backbone:pQF; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23995928
Proper citation: RRID:Addgene_48098 Copy
Genetic Insert: E. coli lacZ
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7300; Vector Backbone:pQF; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23995928
Proper citation: RRID:Addgene_48094 Copy
Genetic Insert: E. coli Conjugation genes
Vector Backbone Description: Vector Backbone:RK24; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25188631
Comments: LB Lennox with Tetracycline to maintain plasmid, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media.
Proper citation: RRID:Addgene_51950 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_205019 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_205017 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pMflT-o4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Comments: The user should always streak the culture on plate and work with isolated fluorescent colonies. Addgene NGS results found mixed population of nucleotides across Term_apFAB390 and C-terminus of NeonGreen, but plasmid function should not be affected.
Proper citation: RRID:Addgene_201855 Copy
Species: Unspecified
Genetic Insert: SAGA p15a His low : T7- His tags low - gfp -p15a tetAp15a - CmTrunc
Vector Backbone Description: Vector Backbone:pSEVAx61; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:40527904
Proper citation: RRID:Addgene_240791 Copy
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