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Species: Synthetic
Genetic Insert: Cys-SpyCatcher002
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:6422; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102829 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:8200; Vector Backbone:PresentER; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/22/267047 for bioRxiv preprint.
Proper citation: RRID:Addgene_102943 Copy
Species: Synthetic
Genetic Insert: Sr22 PI190945
Vector Backbone Description: Vector Backbone:pVecBARII; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_102836 Copy
Species: Synthetic
Genetic Insert: Sr22 PI573523
Vector Backbone Description: Vector Backbone:pVecBARII; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_102837 Copy
Species: Synthetic
Genetic Insert: Sr22 PI573523
Vector Backbone Description: Vector Backbone:pVecBARII; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_102839 Copy
Species: Synthetic
Genetic Insert: Sr22 Schomburgk
Vector Backbone Description: Vector Backbone:pVecBARII; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_102833 Copy
Species: Synthetic
Genetic Insert: Sr22 Schomburgk
Vector Backbone Description: Vector Backbone:pVecBARII; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_102835 Copy
Species: Synthetic
Genetic Insert: SpyCatcher002 EQ
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:6422; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102830 Copy
Species: Synthetic
Genetic Insert: SpyTag002-MBP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102831 Copy
Species: Homo sapiens
Genetic Insert: Tmem192
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102929 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3679; Vector Backbone:pCK01; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_102924 Copy
Species:
Genetic Insert: LmrR-BzdB1_nSP
Vector Backbone Description: Backbone Size:4047; Vector Backbone:pCKTRBS; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_102926 Copy
Species: Other
Genetic Insert: DDdCas9VP192-P300-T2A-GFP-IRES-Neo
Vector Backbone Description: Vector Backbone:PB-CAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102887 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCAP05; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Tetracycline
References:
Comments: pCAP05 can be linearized through the restriction sites ClaI and XhoI. Linear vector can be used for the construction of cluster-specific capture vectors using the same methodology as described for pCAP03.
Proper citation: RRID:Addgene_102920 Copy
Species: Other
Genetic Insert: DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
Vector Backbone Description: Vector Backbone:PB-CAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102888 Copy
Species: Other
Genetic Insert: DDdCas9VP192-T2A-GFP-IRES-Neo
Vector Backbone Description: Vector Backbone:PB-tight; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102889 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc14/mCherry-AID(71-114)-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid can be used to dynamically and conditionally deplete Cdc14 in yeast.
A characterization guide can be found here:
https://www.nature.com/articles/s41598-017-04791-6
Detailed description of plasmid construction can be found in the Supplemental Experimental Procedures of the original paper:
http://www.cell.com/molecular-cell/pdfExtended/S1097-2765(16)30726-2
Proper citation: RRID:Addgene_102884 Copy
Species: Other
Genetic Insert: DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
Vector Backbone Description: Vector Backbone:PB-CAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102886 Copy
Species: Other
Genetic Insert: mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
Vector Backbone Description: Vector Backbone:pSAM; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: The Plac promoter from pGFP-Mut3.1 (Clonetech), along with its associated ribosome binding sequence, was amplified via PCR. Engineered 59 BamHI and 39 NdeI restriction sites were used to sub-clone the resulting fragment into a BamHI/NdeI (New England Biolabs) double digested pSAM_Bt vector upstream of the himar1C9 transposase gene. The kanamycin resistance gene from pKD4 was amplified and ligated using the restriction sites MfeI and XbaI, replacing the erythromycin resistance gene ermG in pSAM_Bt. The resulting transposon mutagenesis vector, pSAM-Ec, was stored and propagated in the pir+ E. coli strain EcS17.
Proper citation: RRID:Addgene_102939 Copy
Species: Mycobacterium bovis BCG
Genetic Insert: pknG
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note: Addgene's quality control sequencing has identified a frameshift mutation near the C-terminus of the pET-15b backbone-resident Lac-I ORF. However, the depositing lab states that this mutation does not affect PknG protein expression.
Proper citation: RRID:Addgene_102814 Copy
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