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Species: Herpers Simplex Virus
Genetic Insert: Thymidine Kinase
Vector Backbone Description: Backbone Size:4456; Vector Backbone:pRS403; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17083135
Proper citation: RRID:Addgene_71789 Copy
Species: Escherichia coli str. K-12 substr. MG1655
Genetic Insert: thioredoxin reductase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71702 Copy
Species: Homo sapiens
Genetic Insert: S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP
Vector Backbone Description: Backbone Size:4246; Vector Backbone:PX462; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26969735
Comments: The catalytic domain of human DNMT3A (amino acids P602-V912) was derived from the plasmid pcDNA3/Myc-DNMT3A (Addgene, plasmid #35521) (Chen et al., 2005, J Cell Biochem 95: 902-917). Undesired BbsI restriction site was removed by site-directed mutagenesis, without affecting the amino acid sequence.
Plasmid pSpCas9n(BB)-2A-Puro (PX462) (Addgene, plasmid #48141) (Ran et al., 2013, Nat Protoc 8: 2281-2308) was used as a backbone. Additional H840A mutation was introduced into Cas9n D10A nickase. T2A-PuroR EcoRI fragment was replaced with T2A-EGFP fragment from pSpCas9n(BB)-2A-GFP (PX461) (Addgene, plasmid #48140) (Ran et al., 2013, Nat Protoc 8: 2281-2308).
Proper citation: RRID:Addgene_71666 Copy
Species: Homo sapiens
Genetic Insert: S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR
Vector Backbone Description: Backbone Size:4246; Vector Backbone:PX462; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26969735
Comments: The catalytic domain of human DNMT3A (amino acids P602-V912) was derived from the plasmid pcDNA3/Myc-DNMT3A (Addgene, plasmid #35521) (Chen et al., 2005, J Cell Biochem 95: 902-917). Undesired BbsI restriction site was removed by site-directed mutagenesis, without affecting the amino acid sequence.
Plasmid pSpCas9n(BB)-2A-Puro (PX462) (Addgene, plasmid #48141) (Ran et al., 2013, Nat Protoc 8: 2281-2308) was used as a backbone. Additional H840A mutation was introduced into Cas9n D10A nickase.
Proper citation: RRID:Addgene_71667 Copy
Species: Acetobacter aceti 1023
Genetic Insert: thioredoxin reductase 1
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71703 Copy
Species: Acetobacter aceti 1023
Genetic Insert: thioredoxin reductase 1
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71704 Copy
Species: Homo sapiens
Genetic Insert: humanized S. pyogenes Cas9 fused to human Geminin
Vector Backbone Description: Backbone Marker:Zhang lab, Addgene plasmid 42230; Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26854237
Proper citation: RRID:Addgene_71707 Copy
Species: Homo sapiens
Genetic Insert: HIF2A
Vector Backbone Description: Vector Backbone:pCDH; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24998849
Proper citation: RRID:Addgene_71708 Copy
Species: Homo sapiens
Genetic Insert: eiF4A1
Vector Backbone Description: Backbone Size:5490; Vector Backbone:pcDNA3.1(+)/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71657 Copy
Genetic Insert: min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM
Vector Backbone Description: Backbone Marker:(GenBank EF362409.1); Backbone Size:8052; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27001518
Comments: The plasmid p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM for expression of FLP-inducible Dam-LAM protein was made by cloning the Drosophila Lamin Dm0 open reading frame from the previously described pDamMyc-Dm0 plasmid (Pickersgill et al., 2006, PMID=16878134) between the AscI and NotI sites in p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] vector.
Proper citation: RRID:Addgene_71811 Copy
Species: Homo sapiens
Genetic Insert: EIF4A2
Vector Backbone Description: Backbone Size:5490; Vector Backbone:pcDNA3.1(+)/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71658 Copy
Genetic Insert: min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC
Vector Backbone Description: Backbone Marker:(GenBank EF362409.1); Backbone Size:8052; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27001518
Comments: The plasmid p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC for expression of FLP-inducible Dam-PC protein was made by cloning the Drosophila Polycomb open reading frame from the previously described pNDamMyc-Pc plasmid (Tolhuis et al., 2006, PMID=16628213) between the AscI and NotI sites in p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] vector
Proper citation: RRID:Addgene_71812 Copy
Species: Arabidopsis thaliana
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Backbone Size:7373; Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26552885
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_71720 Copy
Species: Homo sapiens
Genetic Insert: S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR
Vector Backbone Description: Backbone Size:4246; Vector Backbone:PX462; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26969735
Comments: The catalytic domain of human DNMT3A (amino acids P602-V912) was derived from the plasmid pcDNA3/Myc-DNMT3A (Addgene, plasmid #35521) (Chen et al., 2005, J Cell Biochem 95: 902-917). Undesired BbsI restriction site was removed by site-directed mutagenesis, without affecting the amino acid sequence. The DNMT3A active site motif ENV was mutated to ANV (E756A).
Plasmid pSpCas9n(BB)-2A-Puro (PX462) (Addgene, plasmid #48141) (Ran et al., 2013, Nat Protoc 8: 2281-2308) was used as a backbone. Additional H840A mutation was introduced into Cas9n D10A nickase.
Proper citation: RRID:Addgene_71684 Copy
Species: Homo sapiens
Genetic Insert: S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP
Vector Backbone Description: Backbone Size:4246; Vector Backbone:PX462; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26969735
Comments: The catalytic domain of human DNMT3A (amino acids P602-V912) was derived from the plasmid pcDNA3/Myc-DNMT3A (Addgene, plasmid #35521) (Chen et al., 2005, J Cell Biochem 95: 902-917). Undesired BbsI restriction site was removed by site-directed mutagenesis, without affecting the amino acid sequence. The DNMT3A active site motif ENV was mutated to ANV (E756A).
Plasmid pSpCas9n(BB)-2A-Puro (PX462) (Addgene, plasmid #48141) (Ran et al., 2013, Nat Protoc 8: 2281-2308) was used as a backbone. Additional H840A mutation was introduced into Cas9n D10A nickase. T2A-PuroR EcoRI fragment was replaced with T2A-EGFP fragment from pSpCas9n(BB)-2A-GFP (PX461) (Addgene, plasmid #48140) (Ran et al., 2013, Nat Protoc 8: 2281-2308).
Proper citation: RRID:Addgene_71685 Copy
Species: Homo sapiens
Genetic Insert: HSF1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA 3.1/Myc-His (-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25963659
Proper citation: RRID:Addgene_71724 Copy
Species: Acetobacter aceti 1023
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71689 Copy
Species: Homo sapiens
Genetic Insert: dihydropterine reductase
Vector Backbone Description: Backbone Marker:JBEI; Vector Backbone:pBbE1k; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22948011
Comments: There is a patent for this.
Lee, T.S., Satoh, Y., Keasling, J.D., Host cells and methods for oxidizing aromatic amino acids, PCT/US2012/031025
Proper citation: RRID:Addgene_71722 Copy
Species: Homo sapiens
Genetic Insert: Gal4 DBD HSF1 Δ∼PRD RD VP16 AD
Vector Backbone Description: Backbone Size:2924; Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25963659
Proper citation: RRID:Addgene_71727 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal4 DBD(1-147)
Vector Backbone Description: Backbone Size:2924; Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25963659
Proper citation: RRID:Addgene_71728 Copy
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