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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
p403-BrdU-Inc
 
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1+ mentions
RRID:Addgene_71789 Thymidine Kinase Herpers Simplex Virus Ampicillin PMID:17083135 Backbone Size:4456; Vector Backbone:pRS403; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:09:46 3
pJK651
 
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RRID:Addgene_71702 thioredoxin reductase Escherichia coli str. K-12 substr. MG1655 Ampicillin Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:09:45 0
pdCas9-DNMT3A-EGFP
 
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10+ mentions
RRID:Addgene_71666 S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP Homo sapiens Ampicillin PMID:26969735 The catalytic domain of human DNMT3A (amino acids P602-V912) was derived from the plasmid pcDNA3/Myc-DNMT3A (Addgene, plasmid #35521) (Chen et al., 2005, J Cell Biochem 95: 902-917). Undesired BbsI restriction site was removed by site-directed mutagenesis, without affecting the amino acid sequence. Plasmid pSpCas9n(BB)-2A-Puro (PX462) (Addgene, plasmid #48141) (Ran et al., 2013, Nat Protoc 8: 2281-2308) was used as a backbone. Additional H840A mutation was introduced into Cas9n D10A nickase. T2A-PuroR EcoRI fragment was replaced with T2A-EGFP fragment from pSpCas9n(BB)-2A-GFP (PX461) (Addgene, plasmid #48140) (Ran et al., 2013, Nat Protoc 8: 2281-2308). Backbone Size:4246; Vector Backbone:PX462; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin D10A and H840A in S.pyogenes Cas9 2026-08-29 01:09:45 28
pdCas9-DNMT3A-PuroR
 
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RRID:Addgene_71667 S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR Homo sapiens Ampicillin PMID:26969735 The catalytic domain of human DNMT3A (amino acids P602-V912) was derived from the plasmid pcDNA3/Myc-DNMT3A (Addgene, plasmid #35521) (Chen et al., 2005, J Cell Biochem 95: 902-917). Undesired BbsI restriction site was removed by site-directed mutagenesis, without affecting the amino acid sequence. Plasmid pSpCas9n(BB)-2A-Puro (PX462) (Addgene, plasmid #48141) (Ran et al., 2013, Nat Protoc 8: 2281-2308) was used as a backbone. Additional H840A mutation was introduced into Cas9n D10A nickase. Backbone Size:4246; Vector Backbone:PX462; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin D10A and H840A in S.pyogenes Cas9 2026-08-29 01:09:45 7
pJK394
 
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RRID:Addgene_71703 thioredoxin reductase 1 Acetobacter aceti 1023 Ampicillin Backbone Size:3666; Vector Backbone:pET23a; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-29 01:09:45 0
pJK558
 
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RRID:Addgene_71704 thioredoxin reductase 1 Acetobacter aceti 1023 Ampicillin Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin changes cysteine-138 to serine 2026-08-29 01:09:45 0
pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)
 
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1+ mentions
RRID:Addgene_71707 humanized S. pyogenes Cas9 fused to human Geminin Homo sapiens Ampicillin PMID:26854237 Backbone Marker:Zhang lab, Addgene plasmid 42230; Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:09:45 9
pCDH-CB1-HIF2a-GFP-T2A-Puro
 
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1+ mentions
RRID:Addgene_71708 HIF2A Homo sapiens Ampicillin PMID:24998849 Vector Backbone:pCDH; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 01:09:45 2
pcDNA3.1+ eiF4A1 myc HIS
 
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1+ mentions
RRID:Addgene_71657 eiF4A1 Homo sapiens Ampicillin Backbone Size:5490; Vector Backbone:pcDNA3.1(+)/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin aa403 from Ala to Arg 2026-08-29 01:09:45 2
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM
 
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1+ mentions
RRID:Addgene_71811 min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM Ampicillin PMID:27001518 The plasmid p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM for expression of FLP-inducible Dam-LAM protein was made by cloning the Drosophila Lamin Dm0 open reading frame from the previously described pDamMyc-Dm0 plasmid (Pickersgill et al., 2006, PMID=16878134) between the AscI and NotI sites in p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] vector. Backbone Marker:(GenBank EF362409.1); Backbone Size:8052; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:09:46 1
pcDNA3.1+ eiF4A2 myc HIS
 
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1+ mentions
RRID:Addgene_71658 EIF4A2 Homo sapiens Ampicillin Backbone Size:5490; Vector Backbone:pcDNA3.1(+)/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:09:45 2
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC
 
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1+ mentions
RRID:Addgene_71812 min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC Ampicillin PMID:27001518 The plasmid p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC for expression of FLP-inducible Dam-PC protein was made by cloning the Drosophila Polycomb open reading frame from the previously described pNDamMyc-Pc plasmid (Tolhuis et al., 2006, PMID=16628213) between the AscI and NotI sites in p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] vector Backbone Marker:(GenBank EF362409.1); Backbone Size:8052; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:09:46 1
pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)
 
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1+ mentions
RRID:Addgene_71720 TRANSPORT INHIBITOR RESPONSE 1 Arabidopsis thaliana Ampicillin PMID:26552885 Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3 Backbone Size:7373; Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin codon-optimized for expression in C. elegans; Two point mutations D170E and M473L incorporated 2026-08-29 01:09:45 9
pdCas9-DNMT3A-PuroR (ANV)
 
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1+ mentions
RRID:Addgene_71684 S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR Homo sapiens Ampicillin PMID:26969735 The catalytic domain of human DNMT3A (amino acids P602-V912) was derived from the plasmid pcDNA3/Myc-DNMT3A (Addgene, plasmid #35521) (Chen et al., 2005, J Cell Biochem 95: 902-917). Undesired BbsI restriction site was removed by site-directed mutagenesis, without affecting the amino acid sequence. The DNMT3A active site motif ENV was mutated to ANV (E756A). Plasmid pSpCas9n(BB)-2A-Puro (PX462) (Addgene, plasmid #48141) (Ran et al., 2013, Nat Protoc 8: 2281-2308) was used as a backbone. Additional H840A mutation was introduced into Cas9n D10A nickase. Backbone Size:4246; Vector Backbone:PX462; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin D10A and H840A in S.pyogenes Cas9; E756A inactivating mutation in H. sapiens DNMT3A 2026-08-29 01:09:45 4
pdCas9-DNMT3A-EGFP (ANV)
 
Resource Report
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1+ mentions
RRID:Addgene_71685 S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP Homo sapiens Ampicillin PMID:26969735 The catalytic domain of human DNMT3A (amino acids P602-V912) was derived from the plasmid pcDNA3/Myc-DNMT3A (Addgene, plasmid #35521) (Chen et al., 2005, J Cell Biochem 95: 902-917). Undesired BbsI restriction site was removed by site-directed mutagenesis, without affecting the amino acid sequence. The DNMT3A active site motif ENV was mutated to ANV (E756A). Plasmid pSpCas9n(BB)-2A-Puro (PX462) (Addgene, plasmid #48141) (Ran et al., 2013, Nat Protoc 8: 2281-2308) was used as a backbone. Additional H840A mutation was introduced into Cas9n D10A nickase. T2A-PuroR EcoRI fragment was replaced with T2A-EGFP fragment from pSpCas9n(BB)-2A-GFP (PX461) (Addgene, plasmid #48140) (Ran et al., 2013, Nat Protoc 8: 2281-2308). Backbone Size:4246; Vector Backbone:PX462; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin D10A and H840A in S.pyogenes Cas9; E756A inactivating mutation in H. sapiens DNMT3A 2026-08-29 01:09:45 7
pcDNA-hHSF1 WT
 
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1+ mentions
RRID:Addgene_71724 HSF1 Homo sapiens Ampicillin PMID:25963659 Backbone Size:5400; Vector Backbone:pcDNA 3.1/Myc-His (-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:09:45 3
pJK563
 
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RRID:Addgene_71689 N5-carboxyaminoimidazole ribonucleotide mutase Acetobacter aceti 1023 Ampicillin Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin changes tryptophan-34 to phenylalanine, tyrosine-154 to isoleucine, tryptophan-165 to phenylalanine, additional N-terminal methionine 2026-08-29 01:09:45 0
pBbE1k-3
 
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1+ mentions
RRID:Addgene_71722 dihydropterine reductase Homo sapiens Kanamycin PMID:22948011 There is a patent for this. Lee, T.S., Satoh, Y., Keasling, J.D., Host cells and methods for oxidizing aromatic amino acids, PCT/US2012/031025 Backbone Marker:JBEI; Vector Backbone:pBbE1k; Vector Types:; Bacterial Resistance:Kanamycin 2026-08-29 01:09:45 1
Gal4-VP16-HSF1Δ∼PRD
 
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RRID:Addgene_71727 Gal4 DBD HSF1 Δ∼PRD RD VP16 AD Homo sapiens Ampicillin PMID:25963659 Backbone Size:2924; Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:09:45 0
Gal4-VP16
 
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10+ mentions
RRID:Addgene_71728 Gal4 DBD(1-147) Saccharomyces cerevisiae Ampicillin PMID:25963659 Backbone Size:2924; Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:09:45 16

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