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Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9677319
Comments: This is a mutation of an in vitro site of phosphorylation.
Proper citation: RRID:Addgene_11219 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9115241
Comments: This mutant begins at Rad 2nd ATG and contains a deletion of the calmodulin binding domain.
Proper citation: RRID:Addgene_11225 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9115241
Comments: This mutant starts at the second methionine of Rad at residue 40. This mutant corresponds to the GST fusion construct of Rad used by Zhu and Moyers which begins at Rad 2nd ATG.
Proper citation: RRID:Addgene_11224 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9677319
Comments: This is a mutation of an in vitro site of phosphorylation.
Proper citation: RRID:Addgene_11221 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9677319
Comments: This is a mutation of an in vitro site of phosphorylation.
Proper citation: RRID:Addgene_11222 Copy
Species: Homo sapiens
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:#21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110421 Copy
Species: Homo sapiens
Genetic Insert: Pro-SBP-mGFP-COL1A1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8102; Vector Backbone:pLVX-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30587510
Comments: See attached PDF for full descritions
Proper citation: RRID:Addgene_110726 Copy
Species: Mus musculus
Genetic Insert: Rlf
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pbabepuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15950903
Proper citation: RRID:Addgene_11111 Copy
Species: Homo sapiens
Genetic Insert: hTERT, p53 dominant negative
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16267004
Comments: This plasmid was generated by cloning FLAG-hTERT cDNA with flanking EcoRI-SalI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site p53DD cDNA in which HindIII/ClaI sites were again added by PCR.
Proper citation: RRID:Addgene_11128 Copy
Species: Homo sapiens
Genetic Insert: Cyclin D1, Cyclin Dependent Kinase 4
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16267004
Comments: This plasmid was generated by cloning cyclinD1 cDNA with flanking EcoRI-SalI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site CDK4R24C cDNA in which HindIII/ClaI sites were again added by PCR.
Proper citation: RRID:Addgene_11129 Copy
Species: Homo sapiens
Genetic Insert: c-myc, HRas
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16267004
Comments: Contains mouse c-myc and human Hras. This plasmid was generated by cloning c-MycT58A cDNA with flanking BamHI-EcoRI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site H-RasG12V cDNA in which HindIII/ClaI sites were again added by PCR.
Proper citation: RRID:Addgene_11130 Copy
Vector Backbone Description: Backbone Size:5891; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_120308 Copy
Species: Homo sapiens
Genetic Insert: KRASG12V
Vector Backbone Description: Backbone Size:6213; Vector Backbone:mycBioID-pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30194290
Proper citation: RRID:Addgene_122211 Copy
Species: Homo sapiens
Genetic Insert: HRASG12V
Vector Backbone Description: Backbone Size:6213; Vector Backbone:mycBioID-pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30194290
Proper citation: RRID:Addgene_122214 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116233 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS415; Vector Types:Yeast Expression, Other, Gateway Destination; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14193 Copy
Species: E.coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbbr; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:16207908
Comments: Use gentamicin at 10 ug/mL.
Proper citation: RRID:Addgene_14467 Copy
Genetic Insert: Lineage tracing library
Vector Backbone Description: Backbone Marker:Allon Klein; Vector Backbone:pLARRY-EGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31974159
Proper citation: RRID:Addgene_140024 Copy
Species: Homo sapiens
Genetic Insert: FTO and inactive dCas9
Vector Backbone Description: Backbone Size:3421; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31383972
Proper citation: RRID:Addgene_134782 Copy
Species: Homo sapiens
Genetic Insert: hGADD45A-PUFa-hTET1CD(1418-2136)
Vector Backbone Description: Backbone Marker:Plasmid #25896; Backbone Size:9573; Vector Backbone:pLX302; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134837 Copy
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