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Species: Acetobacter aceti 1023
Genetic Insert: succinyl-CoA:acetate CoA-transferase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23030530
Comments: Much higher protein production is achieved by co-production with E. coli GroEL/GroES.
Proper citation: RRID:Addgene_71578 Copy
Species: Acetobacter aceti 1023
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71612 Copy
Species: Acetobacter aceti 1023
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71618 Copy
Species: Acetobacter aceti 1023
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The protein produced has no detectable catalytic activity.
Proper citation: RRID:Addgene_71615 Copy
Species: Treponema denticola
Genetic Insert: phosphoribosylaminoimidazole carboxylase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase
Proper citation: RRID:Addgene_71642 Copy
Species: Treponema denticola
Genetic Insert: phosphoribosylaminoimidazole carboxylase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase
Proper citation: RRID:Addgene_71643 Copy
Species: Treponema denticola
Genetic Insert: phosphoribosylaminoimidazole carboxylase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase
Proper citation: RRID:Addgene_71640 Copy
Species: Treponema denticola
Genetic Insert: phosphoribosylaminoimidazole carboxylase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase
Proper citation: RRID:Addgene_71641 Copy
Species: Treponema denticola
Genetic Insert: phosphoribosylaminoimidazole carboxylase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The protein produced is anticipated to have no catalytic activity.
This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase
Proper citation: RRID:Addgene_71647 Copy
Species: Thermoplasma acidophilum DSM 1728
Genetic Insert: citrate synthase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26457521
Proper citation: RRID:Addgene_71591 Copy
Species: Acetobacter aceti 1023
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71594 Copy
Vector Backbone Description: Backbone Size:2200; Vector Backbone:pWV01; Vector Types:integration vector for bacteria; Bacterial Resistance:Erythromycin
Defining Citation: PMID:9003306
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
This plasmid has been found to be somewhat prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_71595 Copy
Species: Acetobacter aceti 1023
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71596 Copy
Species: Treponema denticola
Genetic Insert: phosphoribosylaminoimidazole carboxylase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase
Proper citation: RRID:Addgene_71630 Copy
Species: Treponema denticola
Genetic Insert: phosphoribosylaminoimidazole carboxylase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase
Proper citation: RRID:Addgene_71635 Copy
Species: Treponema denticola
Genetic Insert: phosphoribosylaminoimidazole carboxylase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase
Proper citation: RRID:Addgene_71634 Copy
Species: Treponema denticola
Genetic Insert: phosphoribosylaminoimidazole carboxylase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase
Proper citation: RRID:Addgene_71638 Copy
Vector Backbone Description: Backbone Marker:Broad Insitute; Backbone Size:7709; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Doxycycline inducible; P2A Self Cleaving Peptide; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30795624
Proper citation: RRID:Addgene_71782 Copy
Genetic Insert: EGFP coding sequence followed by eSIBR cassette
Vector Backbone Description: Backbone Size:5352; Vector Backbone:pcDNA3; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26582923
Comments: The original SIBR cassette and instructions for cloning into and chaining SIBR amiRNAs are described in:
Chung, Kwan-Ho, et al. "Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155." Nucleic Acids Research 34.7 (2006): e53-e53.
Proper citation: RRID:Addgene_71664 Copy
Species: Homo sapiens
Genetic Insert: hnRNPK
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5490; Vector Backbone:pcDNA3.1(+)/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71662 Copy
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