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Species: Gallus gallus
Genetic Insert: Cyto-Ovalbumin
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving OVA-T2A-EGFP under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124673 Copy
Species: Gallus gallus
Genetic Insert: Cyto-Ovalbumin
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving OVA-T2A-EGFP under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124676 Copy
Species: Gallus gallus
Genetic Insert: Cyto-Ovalbumin
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving OVA-T2A-EGFP under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124677 Copy
Species: Gallus gallus
Genetic Insert: Cyto-Ovalbumin
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving OVA-T2A-EGFP under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124674 Copy
Species: Gallus gallus
Genetic Insert: Cyto-Ovalbumin
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving OVA-T2A-EGFP under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124675 Copy
Species: Gallus gallus
Genetic Insert: SEMA3A (chick)
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:3000; Vector Backbone:pBLUESCRIPT (SK); Vector Types:pBlue for ISH; Bacterial Resistance:Ampicillin
Comments: To generate the sense probe for ISH, linearize the plasmid with XbaI and transcribe with T3 RNA polymerase. To generate the antisense probe, linearize the plasmid with NotI and transcribe with T7 RNA polymerase.
Please note that T7 is the 5' sequencing primer for the vector; however, the SEMA3A insert is cloned in reverse orientation. For cloning purposes, NotI is at the 5' end of SEMA3A while XbaI is at the 3' end of SEMA3A.
Proper citation: RRID:Addgene_29564 Copy
Species: Gallus gallus
Genetic Insert: TUBB3
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:cDNA for in situ hybridization; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12486167
Comments: For riboprobes, cut with EagI. Runoffs with T7.
Proper citation: RRID:Addgene_31232 Copy
Species: Gallus gallus
Genetic Insert: SHH
Vector Backbone Description: Backbone Marker:A. D. Johnson, P. A. Krieg, Dev. Genet. 17, 280 (1995); Vector Backbone:pXeX; Vector Types:Xenopus expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11251119
Proper citation: RRID:Addgene_31233 Copy
Species: Gallus gallus
Genetic Insert: DISP1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII-TOPO; Vector Types:cDNA for in situ hybridization; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:16246323
Comments: For riboprobe cut with SpeI. Runoffs with T7 polymerase. This is not full length ORF.
Proper citation: RRID:Addgene_31235 Copy
Species: Gallus gallus
Genetic Insert: OVA
Vector Backbone Description: Backbone Marker:Reff et al., 1994; Backbone Size:9209; Vector Backbone:TCAE (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9364701
Comments: *The backbone (TCAE) was modified to eliminate the immunoglobulin variable and constant regions, the neomycin resistance gene, and the DHFR gene. See paper for more details.
Proper citation: RRID:Addgene_31598 Copy
Species: Gallus gallus
Genetic Insert: capping protein alpha 1 beta 1 (deletion 28 amino acids )
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:5000; Vector Backbone:pET- 3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12956956
Comments: Soeno et al 1998; journal of muscle research and cell motility 19, 639-646
Proper citation: RRID:Addgene_13300 Copy
Species: Gallus gallus
Genetic Insert: capping protein alpha 1 (deletion 28 amino acids) beta 1 subunits
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:5000; Vector Backbone:pET- 3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12956956
Comments: Soeno et al 1998; journal of muscle research and cell motility 19, 639-646
Proper citation: RRID:Addgene_13299 Copy
Species: Gallus gallus
Genetic Insert: PLA2G4A-C2 domain
Vector Backbone Description: Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31050338
Proper citation: RRID:Addgene_170753 Copy
Species: Gallus gallus
Genetic Insert: 1XDR4
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Comments: Note that there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences should not affect plasmid function.
Proper citation: RRID:Addgene_32614 Copy
Species: Gallus gallus
Genetic Insert: Thyroid Response Element
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32612 Copy
Species: Gallus gallus
Genetic Insert: rGH-PAL
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32617 Copy
Species: Gallus gallus
Genetic Insert: GAPDH shRNA #2
Vector Backbone Description: Vector Backbone:pRFP RNAiC-1; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32622 Copy
Species: Gallus gallus
Genetic Insert: TRbeta shRNA #1
Vector Backbone Description: Vector Backbone:pRFP RNAiC-1; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32620 Copy
Species: Gallus gallus
Genetic Insert: ASIC1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_32791 Copy
Species: Gallus gallus
Genetic Insert: AP-SEMA3C
Vector Backbone Description: Backbone Marker:modified from PCDNA3 see methods in associated publication.; Backbone Size:6000; Vector Backbone:pAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9331347
Proper citation: RRID:Addgene_29449 Copy
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