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Species: Acetobacter aceti 1023
Genetic Insert: alanine racemase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16843006
Comments: This plasmid contains 49 additional nucleotides between the NdeI cloning site and the initiator Met codon.
Proper citation: RRID:Addgene_71552 Copy
Species: Acetobacter aceti 1023
Genetic Insert: alanine racemase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16843006
Proper citation: RRID:Addgene_71559 Copy
Species: Acetobacter aceti 1023
Genetic Insert: alanine racemase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16843006
Comments: This plasmid is described as being cloned from a PCR product in the paper. It was actually produced by subcloning the NdeI-HindIII fragment from pJK303.
Proper citation: RRID:Addgene_71556 Copy
Species: Homo sapiens
Genetic Insert: Septin 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22608511
Proper citation: RRID:Addgene_71583 Copy
Species: Acetobacter aceti 1023
Genetic Insert: succinyl-CoA:acetate CoA-transferase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23030530
Comments: Much higher protein production is achieved by co-production with E. coli GroEL/GroES.
Proper citation: RRID:Addgene_71581 Copy
Genetic Insert: acetyl-CoA:oxalate CoA-transferase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23935849
Proper citation: RRID:Addgene_71587 Copy
Genetic Insert: acetyl-CoA:oxalate CoA-transferase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23935849
Proper citation: RRID:Addgene_71588 Copy
Species: Acetobacter aceti 1023
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71621 Copy
Genetic Insert: acetyl-CoA:oxalate CoA-transferase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23935849
Proper citation: RRID:Addgene_71585 Copy
Species: Treponema denticola
Genetic Insert: phosphoribosylaminoimidazole carboxylase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase
Proper citation: RRID:Addgene_71628 Copy
Species: Treponema denticola
Genetic Insert: phosphoribosylaminoimidazole carboxylase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase
Proper citation: RRID:Addgene_71629 Copy
Species: Treponema denticola
Genetic Insert: phosphoribosylaminoimidazole carboxylase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase
Proper citation: RRID:Addgene_71626 Copy
Species: Treponema denticola
Genetic Insert: phosphoribosylaminoimidazole carboxylase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase
Proper citation: RRID:Addgene_71627 Copy
Species: Thermoplasma acidophilum DSM 1728
Genetic Insert: citrate synthase
Vector Backbone Description: Vector Backbone:pRec7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19645419
Comments: Protein production is induced using nalidixic acid as described. (Kurz, L. C., Drysdale, G., Riley, M., Tomar, M. A., Chen, J., Russell, R. J. M., and Danson, M. J. ( 2000) Kinetics and mechanism of the citrate synthase from the thermophilic archaeon Thermoplasma acidophilum Biochemistry 39, 2283– 2296 DOI: 10.1021/bi991982r)
Also known as pJK383
Proper citation: RRID:Addgene_71573 Copy
Species: Acetobacter aceti 1023
Genetic Insert: putative CoA-transferase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18502856
Proper citation: RRID:Addgene_71570 Copy
Species: Acetobacter aceti 1023
Genetic Insert: acetyl-CoA:oxalate CoA-transferase
Vector Backbone Description: Backbone Size:3663; Vector Backbone:pET23d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18502856
Proper citation: RRID:Addgene_71571 Copy
Species: Acetobacter aceti 1023
Genetic Insert: succinyl-CoA:acetate CoA-transferase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23030530
Comments: Much higher protein production is achieved by co-production with E. coli GroEL/GroES.
Proper citation: RRID:Addgene_71576 Copy
Species: Acetobacter aceti 1023
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The protein produced has no detectable catalytic activity.
Proper citation: RRID:Addgene_71610 Copy
Species: Acetobacter aceti 1023
Genetic Insert: succinyl-CoA:acetate CoA-transferase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23030530
Comments: Much higher protein production is achieved by co-production with E. coli GroEL/GroES.
Proper citation: RRID:Addgene_71575 Copy
Species: Acetobacter aceti 1023
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The protein produced has no detectable catalytic activity.
Proper citation: RRID:Addgene_71613 Copy
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