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Genetic Insert: NuMA(1-2115ΔNLS)-RFP-Nano
Vector Backbone Description: Vector Backbone:pMK247_addgene#105926; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29848445
Comments: Please note that some discrepancies were found between Addgene's quality control result and the depositor's full plasmid sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_114699 Copy
Genetic Insert: NuMA(1-2115)-RFP-Nano
Vector Backbone Description: Vector Backbone:pMK247_addgene#105926; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29848445
Comments: Please note that some discrepancies were found between Addgene's quality control result and the depositor's full plasmid sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_114698 Copy
Species: Mus musculus
Genetic Insert: sgRNA M9
Vector Backbone Description: Vector Backbone:pT2K-CAGGS-IRES-CFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29880921
Proper citation: RRID:Addgene_114731 Copy
Species: Mus musculus
Genetic Insert: sgRNA M7
Vector Backbone Description: Vector Backbone:pT2K-CAGGS-IRES-CFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29880921
Proper citation: RRID:Addgene_114730 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G07550
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: SLDCGLASNESPYNEANSNLTYISDADFIQGGKTGNVQKDLLMKLRKPYTVLRYFPDGIRNCYSLNVKQDTNYLIRVMFRYGNYDGLNNSPRFDLYLGPNIWTTIDMGKSGDGVLEEIIHITRSNILDICLVKTGTSTPMISSIELRPLLYDTYIAQTGSLRNYNRFYFTDSNNYIRYPQDVHDRIWVPLILPEWTHINTSHHVIDSIDGYDPPQDVLRTGAMPANASDPMTITWNLKTATDQVYGYIYIAEIMEVQANETREFEVVVNNKVHFDPFRPTRFEAQVMFNNVPLTCEGGFCRLQLIKTPKSTLPPLMNAFEIFTGIEFPQSETNQNDVIAVKNIQASYGLNRISWQGDPCVPKQFLWTGLSCNVIDVSTPPRIVKLDLSSSGLNGVIPPSIQNLTQLQELDLSQNNLTGKVPEFLAKMKYLLVINLSGNKLSGLVPQALLDRKKEGLKLLVDENMICVSCGT
Proper citation: RRID:Addgene_114738 Copy
Species: Synthetic
Genetic Insert: sfGFP Strands 1-9
Vector Backbone Description: Backbone Size:4362; Vector Backbone:pETARA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29671583
Proper citation: RRID:Addgene_114680 Copy
Genetic Insert: RFP-Nano
Vector Backbone Description: Vector Backbone:pUC-57; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29848445
Proper citation: RRID:Addgene_114688 Copy
Genetic Insert: NuMA sgRNA
Vector Backbone Description: Vector Backbone:pX330_Addgene#42230; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29848445
Proper citation: RRID:Addgene_114687 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125495
Comments: Fragments contain 6 bp barcode TAGGAC at 3' end after digestion with MylI and ZraI
Proper citation: RRID:Addgene_114720 Copy
Species: Medaka fish (Oryzias latipes)
Genetic Insert: Tol2 transposase
Vector Backbone Description: Backbone Marker:Koichi Kawakami; Vector Backbone:pCAGGS-T2TP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28504719
Proper citation: RRID:Addgene_114725 Copy
Species: Mus musculus
Genetic Insert: sgRNA M5, sgRNA M7, sgRNA M9
Vector Backbone Description: Vector Backbone:pT2K-CAGGS-IRES-CFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29880921
Proper citation: RRID:Addgene_114729 Copy
Genetic Insert: sgRNA M5
Vector Backbone Description: Vector Backbone:pT2K-CAGGS-IRES-CFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29880921
Proper citation: RRID:Addgene_114728 Copy
Genetic Insert: stuffer (infusion)
Vector Backbone Description: Vector Backbone:pT2K-CAGGS-T2TP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29880921
Proper citation: RRID:Addgene_114727 Copy
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4895; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32427827
Proper citation: RRID:Addgene_114670 Copy
Species: Mus musculus
Genetic Insert: Otopetrin 1
Vector Backbone Description: Backbone Size:3019; Vector Backbone:pGEMHE; Vector Types:For generating the mRNA, which can highly express in Xenopus laevis oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29371428
Proper citation: RRID:Addgene_114674 Copy
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:4508; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_114671 Copy
Species: Mus musculus
Genetic Insert: Otopetrin 2
Vector Backbone Description: Backbone Size:5429; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29371428
Proper citation: RRID:Addgene_114678 Copy
Species: Mus musculus
Genetic Insert: Otopetrin 1
Vector Backbone Description: Backbone Size:5429; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29371428
Comments: Note: mOtop1 contains G396A which is a known mutation/SNP
Proper citation: RRID:Addgene_114677 Copy
Species: Mus musculus
Genetic Insert: Otopetrin 2
Vector Backbone Description: Backbone Size:3019; Vector Backbone:pGEMHE; Vector Types:For generating the mRNA, which can highly express in Xenopus laevis oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29371428
Proper citation: RRID:Addgene_114675 Copy
Genetic Insert: LGN sgRNA
Vector Backbone Description: Vector Backbone:pX330_Addgene#42230; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29848445
Proper citation: RRID:Addgene_114715 Copy
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