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Genetic Insert: aminoacyl-tRNA synthetase for a caged cysteine
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pMAH2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25647354
Comments: The aminoacyl-tRNA synthetase insert contains ML42GQ compared to all available reference sequences. The depositor states that these mutations are intended and the plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_71404 Copy
Genetic Insert: Npu DnaE Intein insertion in mCherry
Vector Backbone Description: Backbone Marker:lab constructed; Backbone Size:6400; Vector Backbone:pMAH2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25647354
Proper citation: RRID:Addgene_71405 Copy
Species: Homo sapiens
Genetic Insert: HDAC4*
Vector Backbone Description: Backbone Marker:Addgene plasmid #11160; Backbone Size:4798; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26272629
Proper citation: RRID:Addgene_71402 Copy
Genetic Insert: Src active kinase domain inserted with Npu DnaE intein
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25647354
Proper citation: RRID:Addgene_71406 Copy
Species: Homo sapiens
Genetic Insert: SUMO1/sentrin specific peptidase 7
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5289; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26522917
Proper citation: RRID:Addgene_71470 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.10; Vector Types:Mammalian Expression, humanSTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: Note: The plasmid for enhancer-activity testing by luciferase assays has been used in the Arnold et al. 2013 publication. It should be replaced for all enhancer activity tests mammalian cells with the updated version reported by Muerdter et al. 2017. The improved version is Addgene plasmid # 99297. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017).
Gateway-cassette was added between the KpnI and BglII sites and SCP1 between BglII and HindIII
Proper citation: RRID:Addgene_71510 Copy
Species: Morone americana (Perch)
Genetic Insert: Connexin 35
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA 3.1 zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26359298
Proper citation: RRID:Addgene_71511 Copy
Species: Neisseria meningitidis
Genetic Insert: pMCSG7-Inactive-NmeCas9
Vector Backbone Description: Backbone Marker:MCSG; Backbone Size:5286; Vector Backbone:pMCSG7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26474066
Proper citation: RRID:Addgene_71475 Copy
Species: Morone americana (Perch)
Genetic Insert: Connexin 35
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3961; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26359298
Comments: C-terminal EGFP fusion on Cx35 replaced with HaloTag by cutting with SmaI and NotI and inserting HaloTag cut from pHaloTag-N with XhoI, ends filled with Klenow, and NotI.
Proper citation: RRID:Addgene_71512 Copy
Species: Mus musculus
Genetic Insert: Septin 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15774761
Proper citation: RRID:Addgene_71546 Copy
Species: Mus musculus
Genetic Insert: Septin 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21788367
Proper citation: RRID:Addgene_71549 Copy
Species: Acetobacter aceti 1023
Genetic Insert: thioredoxin
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17192591
Proper citation: RRID:Addgene_71561 Copy
Species: Crepis alpina
Genetic Insert: delta12 fatty acid acetylenase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5857; Vector Backbone:pYES2; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17329262
Proper citation: RRID:Addgene_71562 Copy
Species: Helianthus annuus
Genetic Insert: delta12 fatty acid acetylenase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5857; Vector Backbone:pYES2; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17329262
Proper citation: RRID:Addgene_71566 Copy
Species: Crepis alpina
Genetic Insert: delta12 fatty acid desaturase (form 3)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5857; Vector Backbone:pYES2; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17329262
Proper citation: RRID:Addgene_71564 Copy
Species: Acetobacter aceti 1023
Genetic Insert: putative CoA-transferase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18502856
Proper citation: RRID:Addgene_71569 Copy
Species: Acetobacter aceti 1023
Genetic Insert: formyl-CoA:oxalate CoA-transferase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18502856
Comments: Described briefly in Mullins, Francois & Kappock (2008) J. Bacteriol. 190: 4933 (DOI: 10.1128/JB.00405-08; PMID: 18502856). Described in more detail in Mullins, et al. (2012) Protein Sci. (DOI: 10.1002/pro.2054; PMID: 22374910).
Proper citation: RRID:Addgene_71567 Copy
Species: Acetobacter aceti 1023
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71601 Copy
Species: Acetobacter aceti 1023
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71608 Copy
Species: Acetobacter aceti 1023
Genetic Insert: alanine racemase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16843006
Proper citation: RRID:Addgene_71555 Copy
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