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Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7464; Vector Backbone:pEF-DEST51; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29401 Copy
Species: Mus musculus
Genetic Insert: SEMA3A
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:3000; Vector Backbone:pBLUESCRIPT (SK); Vector Types:for ISH; Bacterial Resistance:Ampicillin
References:
Comments: Please note that this vector was designed to generate a probe for in situ hybridization (ISH) and is functional for this purpose. The SEMA3A sequence has not been verified to be full-length and may contain additional mutations when compared to the GenBank reference sequence.
Proper citation: RRID:Addgene_29450 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-5X-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29350 Copy
Species: Homo sapiens
Genetic Insert: E2F2 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK2 (Promega); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29469 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGW1myc2b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29347 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA3.1/GS; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments:
Proper citation: RRID:Addgene_29345 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4492; Vector Backbone:pMV261; Vector Types:Bacterial Expression, Mycobacteria expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Replicative mycobacterial destination vector for recombination cloning.
Please note that there are multiple discrepancies between Addgene's quality control sequence and the depositor's theoretical sequence. The Hmama lab assures us that these differences should not affect plasmid function.
Proper citation: RRID:Addgene_29463 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4445; Vector Backbone:pMV361; Vector Types:Bacterial Expression, Mycobacteria expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Integrative mycobacterial destination vector for recombination cloning.
Please note that there are multiple discrepancies between Addgene's quality control sequence and the depositor's theoretical sequence. The Hmama lab assures us that these differences should not affect plasmid function.
Proper citation: RRID:Addgene_29462 Copy
Species: Homo sapiens
Genetic Insert: Cent2
Vector Backbone Description: Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Centrin-2 sequence in this plasmid contains an additional serine at position 3 when compared to GenBank NP_004335.1
Proper citation: RRID:Addgene_29559 Copy
Species: Homo sapiens
Genetic Insert: ADAMTS18
Vector Backbone Description: Backbone Marker:Systems Biosciences Inc; Backbone Size:5500; Vector Backbone:pCDF1-EF1-MCS2-Puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29558 Copy
Species: Homo sapiens
Genetic Insert: ADAMTS18
Vector Backbone Description: Backbone Marker:Systems Biosciences Inc; Backbone Size:5500; Vector Backbone:pCDF1-EF1-MCS2-Puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29556 Copy
Species: Homo sapiens
Genetic Insert: ADAMTS18
Vector Backbone Description: Backbone Marker:Systems Biosciences Inc; Backbone Size:5500; Vector Backbone:pCDF1-EF1-MCS2-Puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29555 Copy
Species: Homo sapiens
Genetic Insert: FUS-267-526aa
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid # 14227); Backbone Size:8126; Vector Backbone:pAG426Gal-ccdB-EYFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29605 Copy
Species: Homo sapiens
Genetic Insert: FUS-101-526aa
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid # 14227); Backbone Size:8126; Vector Backbone:pAG426Gal-ccdB-EYFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29603 Copy
Species: Homo sapiens
Genetic Insert: FUS-1-501aa
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid # 14227); Backbone Size:8126; Vector Backbone:pAG426Gal-ccdB-EYFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29601 Copy
Species: Homo sapiens
Genetic Insert: FUS-1-453aa
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid # 14227); Backbone Size:8126; Vector Backbone:pAG426Gal-ccdB-EYFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29600 Copy
Species: Gallus gallus
Genetic Insert: SEMA3A (chick)
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:3000; Vector Backbone:pBLUESCRIPT (SK); Vector Types:pBlue for ISH; Bacterial Resistance:Ampicillin
References:
Comments: To generate the sense probe for ISH, linearize the plasmid with XbaI and transcribe with T3 RNA polymerase. To generate the antisense probe, linearize the plasmid with NotI and transcribe with T7 RNA polymerase.
Please note that T7 is the 5' sequencing primer for the vector; however, the SEMA3A insert is cloned in reverse orientation. For cloning purposes, NotI is at the 5' end of SEMA3A while XbaI is at the 3' end of SEMA3A.
Proper citation: RRID:Addgene_29564 Copy
Species: Homo sapiens
Genetic Insert: ERBB4
Vector Backbone Description: Backbone Marker:Systems Biosciences Inc; Backbone Size:5500; Vector Backbone:pCDF1-EF2-MCS2-Puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29539 Copy
Species: Homo sapiens
Genetic Insert: ERBB4
Vector Backbone Description: Backbone Marker:Systems Biosciences Inc; Backbone Size:5500; Vector Backbone:pCDF1-EF2-MCS2-Puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29538 Copy
Species: Homo sapiens
Genetic Insert: ERBB4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29530 Copy
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