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Species: Homo sapiens
Genetic Insert: Fgß peptide, ddFLN4 fingerprint
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101239 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant G582A/V583A/E584A/D585A/N588A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101233 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant F491A/M495A/T496A/N497A/Q926A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101235 Copy
Species: Homo sapiens
Genetic Insert: FFSARG peptide, ddFLN4 fingerprint
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101240 Copy
Species: Synthetic
Genetic Insert: LOV2 and STIM1 cytosolic fragment
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101245 Copy
Species: Mus musculus
Genetic Insert: Rock2
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101296 Copy
Species: Other
Genetic Insert: NL4-3 vpu
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101330 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: The TS module with mTFP1-F40-venusA206K was adapted from Vinculin TS (a kind gift from Martin Schwartz [Grashoff et al., 2010]). The TSmod was cloned in to pEGFP-N1 vector at Xho1 and Not1 restriction sites. For aE-Cat-TS, the mouse N-terminal (1_697 aa) of alpha-catenin was inserted before mTFP1 by in- fusion cloning (Takara Bioscience, Clontech) at the Xho1 restriction site
Proper citation: RRID:Addgene_101298 Copy
Species: Mus musculus
Genetic Insert: Rock1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101292 Copy
Species: Mus musculus
Genetic Insert: Rock1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101293 Copy
Species: Mus musculus
Genetic Insert: Rock1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101294 Copy
Species: Other
Genetic Insert: GFP, tat
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101338 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: The C-terminal fragment (698-906 aa) of alpha-catenin was cloned after VenusA206K at the Not1 restriction site
Proper citation: RRID:Addgene_101299 Copy
Species: Other
Genetic Insert: NL4-3 envFS
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101333 Copy
Species: Other
Genetic Insert: NL4-3 envFS
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101334 Copy
Species: Other
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pNL4-3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101341 Copy
Species: Other
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pNL4-4; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: The last methionine in Gag (M423) was left as it was outside the ApaI site used to clone in the mutations. However, C-terminus of Gag is functionally inert, and Pol slips 27 nucleotides away and is unlikely to produce a stable protein product.
Proper citation: RRID:Addgene_101342 Copy
Species: Other
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101349 Copy
Species: Other
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101343 Copy
Species: Other
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pNL4-4; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101344 Copy
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