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Genetic Insert: Cas14b2
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30337455
Proper citation: RRID:Addgene_112504 Copy
Genetic Insert: Cas14a1
Vector Backbone Description: Vector Backbone:pTet; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30337455
Proper citation: RRID:Addgene_112501 Copy
Vector Backbone Description: Vector Backbone:pBAT4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8660525
Proper citation: RRID:Addgene_112589 Copy
Genetic Insert: Cas14b1
Vector Backbone Description: Vector Backbone:pMBP; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30337455
Proper citation: RRID:Addgene_112505 Copy
Genetic Insert: Cas14b2
Vector Backbone Description: Vector Backbone:pMBP; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30337455
Proper citation: RRID:Addgene_112506 Copy
Vector Backbone Description: Vector Backbone:pOP2S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains N-terminal 8xHis, fusion partner: TF-Sumo (Trigger factor from E. coli (UniProtKB: P0A850) linked to Sumo (Smt3 from Saccharomyces cerevisiae, UniProtKB: Q12306), PMID: 26297996) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII
Proper citation: RRID:Addgene_112576 Copy
Vector Backbone Description: Vector Backbone:pOP2S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains N-terminal 8xHis, fusion partner: Trx (thioredoxin (trxA) from E. coli, UniProtKB: P0AA25, PMID: 7763371) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII
Proper citation: RRID:Addgene_112577 Copy
Vector Backbone Description: Vector Backbone:pOP2S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains N-terminal 8xHis, fusion partner: Sumo (Smt3 from Saccharomyces cerevisiae, UniProtKB: Q12306, PMID: 16084395) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII
Proper citation: RRID:Addgene_112574 Copy
Vector Backbone Description: Vector Backbone:pOP2S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains N-terminal 8xHis, fusion partner: TF (Trigger factor (tig) from E. coli, UniProtKB: P0A850, PMID: 18476832) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII
Proper citation: RRID:Addgene_112575 Copy
Vector Backbone Description: Vector Backbone:pOP2S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains N-terminal 8xHis, fusion partner: DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli, UniProtKB: P0AEG4) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII
Proper citation: RRID:Addgene_112562 Copy
Vector Backbone Description: Vector Backbone:pOP2S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains N-terminal 8xHis, fusion partner: AFV (Acidianus Filamentous Virus 1 protein ORF-99, UniProtKB: Q70LE8, PMID: 19472363) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII
Proper citation: RRID:Addgene_112560 Copy
Vector Backbone Description: Vector Backbone:pOP2S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains N-terminal 8xHis, fusion partner: GST (Glutathione S-transferase from Schistosoma japonicum, UniProtKB: P08515, PMID: 3047011) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII
Proper citation: RRID:Addgene_112566 Copy
Vector Backbone Description: Vector Backbone:pOP2S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains N-terminal 8xHis, fusion partner: DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli, active site cysteines are mutated to serines; UniProtKB: P0AEG4, PMID: 9518456) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII
Proper citation: RRID:Addgene_112563 Copy
Vector Backbone Description: Vector Backbone:pOP2S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains N-terminal 8xHis, fusion partner: IF2 (domain I of translation initiation factor IF-2 from E. coli, UniProtKB: P0A705, PMID: 14965771) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII
Proper citation: RRID:Addgene_112567 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Backbone Marker:Ambion; Vector Backbone:pmiR-report; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24297048
Comments: clone into pmiR-report using SacI/HindIII
Proper citation: RRID:Addgene_112543 Copy
Species: Homo sapiens
Genetic Insert: RIPK1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_112491 Copy
Vector Backbone Description: Backbone Size:5270; Vector Backbone:pRS410; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: pRS400 + pRS415 --> pRS410. Insert is KanMX (G418 resistance) from AatII-cut pRS400. Backbone is ApaLI-cut pRS415 (CEN plasmid).
Proper citation: RRID:Addgene_11258 Copy
Vector Backbone Description: Backbone Size:5071; Vector Backbone:pRS418; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: p4339 + pRS420 --> pRS428. Insert is NatMX (clonNAT resistance) from EcoRI-cut p4339. Backbone is NcoI-cut pRS420 (2 micron plasmid).
Proper citation: RRID:Addgene_11257 Copy
Species: Homo sapiens
Genetic Insert: RIPK1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_112490 Copy
Species: Homo sapiens
Genetic Insert: RIPK3
Vector Backbone Description: Vector Backbone:pCINEO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_112495 Copy
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