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Species: Homo sapiens
Genetic Insert: Ple138
Vector Backbone Description: Backbone Size:17590; Vector Backbone:pEMS1040; Vector Types:Pleiades Promoter Project [sic, Pleaides Plieades]; Bacterial Resistance:Ampicillin
References:
Comments: Insert was not sequence confirmed, ends may be different and may contain some internal SNPs (sequence is based on initial cloning design, and not necessarirly final cloning design)
Please note that Addgene's sequencing results with the oEMS2493 primer differ from the full sequence information provided by the depositing laboratory by a 3 nucleotide mismatch at bp# 179-182. This difference is not known to affect the function of the plasmid
Proper citation: RRID:Addgene_28998 Copy
Species: Homo sapiens
Genetic Insert: ER beta
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_28237 Copy
Species: Homo sapiens
Genetic Insert: Ple135
Vector Backbone Description: Backbone Size:17590; Vector Backbone:pEMS1040; Vector Types:Pleiades Promoter Project [sic, Pleaides Plieades]; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28996 Copy
Species: Homo sapiens
Genetic Insert: Ple136
Vector Backbone Description: Backbone Size:17590; Vector Backbone:pEMS1040; Vector Types:Pleiades Promoter Project [sic, Pleaides Plieades]; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28997 Copy
Species: Homo sapiens
Genetic Insert: Androgen receptor
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Addgene's sequencing result indicates a loss of Q58 in the unstable poly-glutamine region. Our sequencing also shows that the BamHI site at the 3' end of the insert was destroyed. Please see our quality control sequence for the most accurate look at this region.
Tilley, W.D., Marcelli, M., Wilson, J.D. and McPhaul, J.M. (1989) Characterization and cloning of a cDNA encoding the human androgen receptor. Proc. Natl Acad. Sci. USA, 86, 327–331
Proper citation: RRID:Addgene_28235 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Backbone Size:4727; Vector Backbone:pEYFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Addgene Plasmid 18712: HA-Ubiquitin
Proper citation: RRID:Addgene_28236 Copy
Species: Homo sapiens
Genetic Insert: l(3)mbt-like 1 (Drosophila)
Vector Backbone Description: Backbone Marker:IBA; Backbone Size:3286; Vector Backbone:pASK-IBA43plus; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28234 Copy
Species: Homo sapiens
Genetic Insert: l(3)mbt-like 2 (Drosophila)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag4A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_28232 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor alpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Tzukerman MT, Esty A, Santiso-Mere D, Danielian P, Parker MG, Stein RB, Pike JW, McDonnell DP 1994 Human estrogen receptor transactivational capacity is determined by both cellular and promoter context and mediated by two functionally distinct intramolecular regions. Mol Endocrinol 8:21–30
Proper citation: RRID:Addgene_28230 Copy
Species: Homo sapiens
Genetic Insert: Tar DNA binding protein
Vector Backbone Description: Backbone Size:5310; Vector Backbone:pCAG-EGFP/RFP-int; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28208 Copy
Species: Homo sapiens
Genetic Insert: Tar DNA binding protein
Vector Backbone Description: Backbone Size:5310; Vector Backbone:pCAG-EGFP/RFP-int; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28207 Copy
Species: Homo sapiens
Genetic Insert: Tar DNA binding protein
Vector Backbone Description: Backbone Size:5310; Vector Backbone:pCAG-EGFP/RFP-int; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28205 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Cellecta; Backbone Size:7500; Vector Backbone:pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28289 Copy
Species: Homo sapiens
Genetic Insert: Ple9
Vector Backbone Description: Backbone Size:17451; Vector Backbone:pEMS1302; Vector Types:Pleiades Promoter Project [sic, Pleaides Plieades]; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29014 Copy
Species: Danio rerio
Genetic Insert: Flrt3 mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See map and Genbank file for plasmid details.
Proper citation: RRID:Addgene_28217 Copy
Species: Mus musculus
Genetic Insert: Oct4, Sox2, Klf4, c-Myc, Lin28
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:10200; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that Addgene's sequencing results using the CMV-F and pCEP-F primers found 2 sequence variants within and after the CMV enhancer. One is a C->G mismatch at position 775 within the CMV enhancer and the second is a deletion at position 949 just after the end of the CAG enhancer, as compared to the full plasmid sequence provided by the depositing scientist. The significance of the two sequence variants is unclear; however, the plasmid functions as described in the associated publication. Addgene's other 4 sequencing results completely matched the depositor's sequence.
Proper citation: RRID:Addgene_28213 Copy
Species: Danio rerio
Genetic Insert: Unc5b mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See map and Genbank file for plasmid details.
Please note that Addgene's sequencing results identified 4 single nucleotide mismatches (at bp# 3373, 3376, 3380 and 3386) when compared to the full plasmid sequence. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen.
Proper citation: RRID:Addgene_28214 Copy
Species:
Genetic Insert: miRNAa
Vector Backbone Description: Backbone Size:6341; Vector Backbone:pCAG-RFP-KX; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28211 Copy
Species:
Genetic Insert: miRNAb
Vector Backbone Description: Backbone Size:6341; Vector Backbone:pCAG-RFP-KX; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28212 Copy
Species: Mus musculus
Genetic Insert: NHERF1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV-2-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28297 Copy
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