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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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ML21 Resource Report Resource Website |
RRID:Addgene_61914 | none | None | PMID:21925267 | Genotype = tyrA hisG Precursor strain = ML14 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Tyr), His Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:00 | 0 | ||
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ML43 Resource Report Resource Website |
RRID:Addgene_61922 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG argH metA lysA thrC asnA asnB Precursor strain = ML42 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met, Lys, Thr, Asn *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:01 | 0 | ||
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YM138 Resource Report Resource Website |
RRID:Addgene_61953 | none | None | PMID:26577727 | Genotype = cysE Precursor strain = C43(DE3) Selective amino acid labeling (and/or requirement) = Cys Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:01 | 0 | ||
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MS1 Resource Report Resource Website |
RRID:Addgene_61960 | none | None | PMID:26577727 | Genotype = cysE hisG Precursor strain = YM138 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = Cys, His Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:01 | 0 | ||
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RF1 Resource Report Resource Website |
RRID:Addgene_61962 | none | None | PMID:26577727 | Genotype = glyA Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Gly Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:01 | 0 | ||
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RF4 Resource Report Resource Website |
RRID:Addgene_62072 | none | None | PMID:26577727 | Genotype = aspC tyrB Precursor strain = RF3 modified from the parent Escherichia coli BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asp++++, Tyr, (Phe) ++++RF4, RF5, and RF13 strains have knockouts in both aspC and tyrB genes and therefore require the presence of L-Asp (but not L-Glu) for growth in M63 minimal medium. These strains can be used for selective labeling of selected aromatic amino acid(s) such as Tyr (RF4, RF5, RF13) and/or Trp (RF13). We have NOT tested if these strains could also be used for selective Asp labeling studies (under investigation). Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:02 | 0 | ||
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RF13 Resource Report Resource Website |
RRID:Addgene_62078 | none | None | PMID:26577727 | Genotype = aspC tyrB trpA trpB Precursor strain = RF4 modified from the parent Escherichia coli BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asp++++, Tyr, Trp, (Phe) ++++RF4, RF5, and RF13 strains have knockouts in both aspC and tyrB genes and therefore require the presence of L-Asp (but not L-Glu) for growth in M63 minimal medium. These strains can be used for selective labeling of selected aromatic amino acid(s) such as Tyr (RF4, RF5, RF13) and/or Trp (RF13). We have NOT tested if these strains could also be used for selective Asp labeling studies (under investigation). Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:02 | 0 | ||
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RF6 Resource Report Resource Website 1+ mentions |
RRID:Addgene_62074 | none | None | PMID:26577727 | Genotype = proC Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Pro Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:02 | 1 | ||
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RF8 Resource Report Resource Website |
RRID:Addgene_62075 | none | None | PMID:26577727 | Genotype = asnA asnB Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asn Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:02 | 0 | ||
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HL5410 Resource Report Resource Website |
RRID:Addgene_62820 | see comments | None | PMID:26261213 | MG1655 + lacIq intergrated at intS + ΔglmZ + Dhfq | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:10 | 0 | ||
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HL 5226 Resource Report Resource Website |
RRID:Addgene_63667 | see comments | None | PMID:26261213 | MG1655 + lacIq intergrated at intS + ΔglmZ | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:20 | 0 | ||
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MG1655 Z1 malE Resource Report Resource Website 1+ mentions |
RRID:Addgene_65915 | MG1655 Z1 malE | None | PMID:26900850 | F-, lambda-, rph-1, laciq, PN25-tetR, SpR, malE- | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:41 | 1 | ||
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SJ358 Resource Report Resource Website |
RRID:Addgene_67755 | none | None | PMID:26251500 | The genotype of the strain relative to MG1655 is complex, since there are many single nucleotide variations and several 10-20kb differences (Brown & Jun (2015) Genome Announcements, Lyons et. al. (2011) PLoS ONE). There are a particular abundance of differences in the rDNA operons, which in several cases align more closely to E. coli strains DH10B, MDS42, and W. Particularly relevant genotypes are: λ+ gal+ eut+ pyrE+ ilvG+ rpoS33Am glnV(SupAm) evgA::IS1 Δrfb | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:58 | 0 | ||
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HL 6703 Resource Report Resource Website |
RRID:Addgene_69778 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | Addgene | 2026-09-26 02:37:15 | 0 | ||||
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HL 6686 Resource Report Resource Website |
RRID:Addgene_69775 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | Addgene | 2026-09-26 02:37:15 | 0 | ||||
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HL 3500 Resource Report Resource Website |
RRID:Addgene_69774 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | Addgene | 2026-09-26 02:37:15 | 0 | ||||
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HL 6778 Resource Report Resource Website |
RRID:Addgene_69782 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | Addgene | 2026-09-26 02:37:15 | 0 | ||||
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HL 6777 Resource Report Resource Website |
RRID:Addgene_69781 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | Addgene | 2026-09-26 02:37:15 | 0 | ||||
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CY027 Resource Report Resource Website |
RRID:Addgene_72402 | None | PMID:26315440 | No antibiotic resistance. | Vector Backbone:E. coli BW25113; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:None | Addgene | 2026-09-26 02:37:39 | 0 | |||
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LC-E03 Resource Report Resource Website |
RRID:Addgene_78552 | pTet--Cas9 cassette integrated at 186 primary attB site | None | PMID:27060147 | Vector Backbone:na; Vector Types:; Bacterial Resistance:None | This is a bacterial strain | Addgene | 2026-09-26 02:38:41 | 0 |
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