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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pTGV-Kan SMR5938
 
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RRID:Addgene_28036 Kanamycin PMID:11483365 Note that Addgene's sequencing results found a 1 nucleotide deletion at bp# 2950 when compared with the sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function. Backbone Size:7157; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-07-25 12:45:24 0
pTGV-CAT SMR5453
 
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RRID:Addgene_28033 Chloramphenicol PMID:11483365 Note that Addgene's sequencing results found a 1 nucleotide mismatch at bp# 238 and 1 nucleotide deletions at bp# 2970 & 3016 when compared with the sequence provided by the depositing laboratory. These differences are not known to affect plasmid function. Backbone Size:7240; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-07-25 12:45:24 0
pJA304
 
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RRID:Addgene_28091 (Gly)5Ala::mCherry(with introns)::tbb-2 3'UTR Caenorhabditis elegans Kanamycin PMID:21637852 Backbone Marker:invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin 2026-07-25 12:45:25 0
pDR-GW AT1.03YEMK
 
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1+ mentions
RRID:Addgene_28004 AT1.03YEMK B. Subtilis Ampicillin PMID:20854260 AT1.03 is composed of mseCFPdeltaC11, in which 11 C-terminal amino acids were truncated, Bacillus subtilis F0F1 ATP synthase subunit epsilon with V9T/V42K/F67T/L78T mutations, and cp173-mVenus. Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin M60Y, G131E, K132M, 133K 2026-07-25 12:45:24 8
pDR-GW AT1.03R122K/R126K
 
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1+ mentions
RRID:Addgene_28005 AT1.03R122K/R126K B. Subtilis Ampicillin PMID:20854260 AT1.03 is composed of mseCFPdeltaC11, in which 11 C-terminal amino acids were truncated, Bacillus subtilis F0F1 ATP synthase subunit epsilon with V9T/V42K/F67T/L78T mutations, and cp173-mVenus. Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin R122K, R126K 2026-07-25 12:45:24 5
pDR-GW FLII12Pglu-700μδ6
 
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1+ mentions
RRID:Addgene_28002 FLII12Pglu-700μδ6 E. Coli Ampicillin PMID:20854260 Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:45:24 1
pDR-GW AT1.03
 
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1+ mentions
RRID:Addgene_28003 AT1.03 B. Subtilis Ampicillin PMID:20854260 AT1.03 is composed of mseCFPdeltaC11, in which 11 C-terminal amino acids were truncated, Bacillus subtilis F0F1 ATP synthase subunit epsilon with V9T/V42K/F67T/L78T mutations, and cp173-mVenus. Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:45:24 6
IL6-GFP
 
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1+ mentions
RRID:Addgene_28088 IL6 Mus musculus Kanamycin PMID:17606866 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-07-25 12:45:25 5
pDR-GW FLIPglu-600μΔ13V
 
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RRID:Addgene_28001 FLIPglu-600μΔ13V E. Coli Ampicillin PMID:20854260 Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin F16A in mglB 2026-07-25 12:45:24 0
NRF2b (nfe212b)_R (OZ562)
 
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RRID:Addgene_28087 Zinc finger array targeting NRF2b (nfe212b) Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene NRF2b (nfe212b) (see below). Please note that this plasmid does NOT contain the NRF2b (nfe212b) sequence. Users must order the complementary plasmid NRF2b (nfe212b)_L (OZ561) [Addgene plasmid 28086] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGAGGAG-3' in a bacterial two hybrid assay, and resulted in 4.34 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin 2026-07-25 12:45:25 0
pEF1a-IRES-Neo
 
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10+ mentions
RRID:Addgene_28019 Ampicillin PMID:20581084 Backbone Size:5633; Vector Backbone:pEF1a-Luc-IRES-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:45:24 14
p15aTetT7mut-fuseYFP
 
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RRID:Addgene_28013 EYFP-fuse, T7 RNAP Synthetic, T7 phage Kanamycin PMID:19801994 Backbone Marker:Clonetech; Backbone Size:2500; Vector Backbone:pProlar.A122; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-07-25 12:45:24 0
pCFPT7
 
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RRID:Addgene_28012 T7 RNAP T7 phage Ampicillin PMID:19801994 Backbone Marker:Novagen; Backbone Size:6000; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:45:24 0
Mfn2-YFP
 
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10+ mentions
RRID:Addgene_28010 mitofusin 2 Homo sapiens Kanamycin PMID:12499352 Santel, A. and M.T. Fuller, Control of mitochondrial morphology by a human mitofusin. J Cell Sci, 2001. 114(Pt 5): p. 867-74. Backbone Size:4700; Vector Backbone:YFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin silent S508S 2026-07-25 12:45:24 14
tdp43-EGFP construct2
 
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RRID:Addgene_28195 Tar DNA binding protein Homo sapiens Kanamycin PMID:21209826 Backbone Marker:Clontech Laboratories, Inc; Backbone Size:4694; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin aa86-414 only 2026-07-25 12:45:26 0
pCR.MCV.Ori339 (589)
 
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RRID:Addgene_28192 MCV replication region Human Merkel Cell polyomavirus Ampicillin and Kanamycin PMID:18812503 Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-07-25 12:45:26 0
FGD5
 
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RRID:Addgene_28109 FGD5:HPC09X-H08:C212499 Homo sapiens Kanamycin PDB: 3MPX. http://www.thesgc.org/structures/3MPX/ Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin contains amino acid residues 889-1304. 2026-07-25 12:45:25 0
BRPF1 (3MO8)
 
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RRID:Addgene_28107 BRPF1:JMC014-E01:C44383 Homo sapiens Kanamycin PDB: 3MO8. https://www.thesgc.org/structures/3MO8/ PDB: 5C6S. https://www.thesgc.org/structures/5C6S Note that this clone is the same as PDB IDs 3MO8 and 5C6S Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-07-25 12:45:25 0
hWIP1 D314A FLAG
 
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1+ mentions
RRID:Addgene_28106 WIP1 Homo sapiens Ampicillin PMID:20118229 Backbone Size:7767; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Contains phosphatase-dead mutation D314A 2026-07-25 12:45:25 3
HA-Cdk9 T186A
 
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RRID:Addgene_28103 Cdk9 Homo sapiens Ampicillin PMID:18829461 Backbone Size:0; Vector Backbone:pcDNA6/3HA; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin Contains T186A mutation in Cdk9 2026-07-25 12:45:25 0

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