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http://www.addgene.org/28036

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7157; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Note that Addgene's sequencing results found a 1 nucleotide deletion at bp# 2950 when compared with the sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function.

Proper citation: RRID:Addgene_28036 Copy   

•   Source: Addgene

http://www.addgene.org/28033

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7240; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Note that Addgene's sequencing results found a 1 nucleotide mismatch at bp# 238 and 1 nucleotide deletions at bp# 2970 & 3016 when compared with the sequence provided by the depositing laboratory. These differences are not known to affect plasmid function.

Proper citation: RRID:Addgene_28033 Copy   

•   Source: Addgene

http://www.addgene.org/28091

Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::mCherry(with introns)::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_28091 Copy   

•   Source: Addgene

http://www.addgene.org/28004

Species: B. Subtilis
Genetic Insert: AT1.03YEMK
Vector Backbone Description: Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: AT1.03 is composed of mseCFPdeltaC11, in which 11 C-terminal amino acids were truncated, Bacillus subtilis F0F1 ATP synthase subunit epsilon with V9T/V42K/F67T/L78T mutations, and cp173-mVenus.

Proper citation: RRID:Addgene_28004 Copy   

•   Source: Addgene

http://www.addgene.org/28005

Species: B. Subtilis
Genetic Insert: AT1.03R122K/R126K
Vector Backbone Description: Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: AT1.03 is composed of mseCFPdeltaC11, in which 11 C-terminal amino acids were truncated, Bacillus subtilis F0F1 ATP synthase subunit epsilon with V9T/V42K/F67T/L78T mutations, and cp173-mVenus.

Proper citation: RRID:Addgene_28005 Copy   

•   Source: Addgene

http://www.addgene.org/28002

Species: E. Coli
Genetic Insert: FLII12Pglu-700μδ6
Vector Backbone Description: Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_28002 Copy   

•   Source: Addgene

http://www.addgene.org/28003

Species: B. Subtilis
Genetic Insert: AT1.03
Vector Backbone Description: Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: AT1.03 is composed of mseCFPdeltaC11, in which 11 C-terminal amino acids were truncated, Bacillus subtilis F0F1 ATP synthase subunit epsilon with V9T/V42K/F67T/L78T mutations, and cp173-mVenus.

Proper citation: RRID:Addgene_28003 Copy   

•   Source: Addgene

http://www.addgene.org/28088

Species: Mus musculus
Genetic Insert: IL6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_28088 Copy   

•   Source: Addgene

http://www.addgene.org/28001

Species: E. Coli
Genetic Insert: FLIPglu-600μΔ13V
Vector Backbone Description: Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_28001 Copy   

•   Source: Addgene

http://www.addgene.org/28087

Species: Danio rerio
Genetic Insert: Zinc finger array targeting NRF2b (nfe212b)
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene NRF2b (nfe212b) (see below). Please note that this plasmid does NOT contain the NRF2b (nfe212b) sequence. Users must order the complementary plasmid NRF2b (nfe212b)_L (OZ561) [Addgene plasmid 28086] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGAGGAG-3' in a bacterial two hybrid assay, and resulted in 4.34 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_28087 Copy   

•   Source: Addgene

http://www.addgene.org/28019

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5633; Vector Backbone:pEF1a-Luc-IRES-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_28019 Copy   

•   Source: Addgene

http://www.addgene.org/28013

Species: Synthetic, T7 phage
Genetic Insert: EYFP-fuse, T7 RNAP
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:2500; Vector Backbone:pProlar.A122; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_28013 Copy   

•   Source: Addgene

http://www.addgene.org/28012

Species: T7 phage
Genetic Insert: T7 RNAP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6000; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_28012 Copy   

•   Source: Addgene

http://www.addgene.org/28010

Species: Homo sapiens
Genetic Insert: mitofusin 2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:YFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Santel, A. and M.T. Fuller, Control of mitochondrial morphology by a human mitofusin. J Cell Sci, 2001. 114(Pt 5): p. 867-74.

Proper citation: RRID:Addgene_28010 Copy   

•   Source: Addgene

http://www.addgene.org/28195

Species: Homo sapiens
Genetic Insert: Tar DNA binding protein
Vector Backbone Description: Backbone Marker:Clontech Laboratories, Inc; Backbone Size:4694; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_28195 Copy   

•   Source: Addgene

http://www.addgene.org/28192

Species: Human Merkel Cell polyomavirus
Genetic Insert: MCV replication region
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_28192 Copy   

•   Source: Addgene

http://www.addgene.org/28109

Species: Homo sapiens
Genetic Insert: FGD5:HPC09X-H08:C212499
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: PDB: 3MPX. http://www.thesgc.org/structures/3MPX/

Proper citation: RRID:Addgene_28109 Copy   

•   Source: Addgene

http://www.addgene.org/28107

Species: Homo sapiens
Genetic Insert: BRPF1:JMC014-E01:C44383
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: PDB: 3MO8. https://www.thesgc.org/structures/3MO8/ PDB: 5C6S. https://www.thesgc.org/structures/5C6S Note that this clone is the same as PDB IDs 3MO8 and 5C6S

Proper citation: RRID:Addgene_28107 Copy   

•   Source: Addgene

http://www.addgene.org/28106

Species: Homo sapiens
Genetic Insert: WIP1
Vector Backbone Description: Backbone Size:7767; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_28106 Copy   

•   Source: Addgene

http://www.addgene.org/28103

Species: Homo sapiens
Genetic Insert: Cdk9
Vector Backbone Description: Backbone Size:0; Vector Backbone:pcDNA6/3HA; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_28103 Copy   

•   Source: Addgene