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Species:
Genetic Insert: FokI endonuclease
Vector Backbone Description: Backbone Size:5725; Vector Backbone:pMLM292; Vector Types:Mammalian Expression, Zebrafish expression; Bacterial Resistance:Ampicillin
References:
Comments: For expression of a ZFN targeted to a site with a 5 or 6 bp spacer and “-” ELD heterodimeric FokI domain. (Doyon et. al,Nat Biotech 2011) in mammalian cells or zebrafish as described by Moore et al., PLoS One 2012 (https://www.ncbi.nlm.nih.gov/pubmed/22655075) and Cade et al., Nucleic Acids Res 2012 (https://www.ncbi.nlm.nih.gov/pubmed/22684503)
Proper citation: RRID:Addgene_28063 Copy
Species: E. coli
Genetic Insert: MutS, MutL
Vector Backbone Description: Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: pSL7 contains a ClaI–BglII fragment of pSL6 (carrying mutS), a BamHI–HindIII fragment of pKC31 (conferring Kanamycin resistance), and a HindIII–PstI fragment of pAL51 (carrying mutL)
Proper citation: RRID:Addgene_28030 Copy
Species: Homo sapiens
Genetic Insert: DUSP7
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLEX-HA-MYC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_27978 Copy
Species: Homo sapiens
Genetic Insert: DUSP7
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLEX-HA-MYC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_27976 Copy
Species: Homo sapiens
Genetic Insert: CIAP2
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid has also been used and described in the following publication: Cellular inhibitor of apoptosis 1 and 2 are ubiquitin ligases for the apoptosis inducer Smac/DIABLO. Hu et al (J Biol Chem. 2003 Mar 21;278(12):10055-60. Epub 2003 Jan 13. https://www.ncbi.nlm.nih.gov/pubmed/12525502 )
Proper citation: RRID:Addgene_27973 Copy
Species: E. coli
Genetic Insert: MutL
Vector Backbone Description: Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: pSL5 contains a PstI–HindIII fragment of pAL51 carrying E. coli mutL and a KanR-conferring HindIII–BamHI fragment of pKC31 replacing digest sites PstI and BamHI of pBR322
Proper citation: RRID:Addgene_28028 Copy
Species: Homo sapiens
Genetic Insert: Daxx
Vector Backbone Description: Backbone Size:4756; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_27974 Copy
Species: Homo sapiens
Genetic Insert: MALT1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_27971 Copy
Species: Homo sapiens
Genetic Insert: CIAP1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid has also been used and described in the following publication: cIAP2 is a ubiquitin protein ligase for BCL10 and is dysregulated in mucosa-associated lymphoid tissue lymphomas. Hu et al (J Clin Invest. 2006 Jan . 116(1):174-81. https://www.ncbi.nlm.nih.gov/pubmed/16395405 )
Proper citation: RRID:Addgene_27972 Copy
Species: Homo sapiens
Genetic Insert: Keap1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:phrGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28025 Copy
Species: Homo sapiens
Genetic Insert: Keap1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28023 Copy
Species: Homo sapiens
Genetic Insert: Ronin
Vector Backbone Description: Backbone Size:5633; Vector Backbone:pEF1a-IRES-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Even though the XbaI site is still present, XbaI does not cut this plasmid anymore, possibly due to methylation.
Proper citation: RRID:Addgene_28021 Copy
Species: Homo sapiens
Genetic Insert: Rubicon
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5078; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28041 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Jie Yang Lab; Backbone Size:3300; Vector Backbone:pLIC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid is used for a novel and straightforward ligation-independent cloning (LIC) method based on nicking DNA endonuclease (NiDE).
To construct a NiDE-compatible pLIC vector, LacZ cDNA was amplified from a modified pMD18 construct by PCR using primers Laz-BgiII-F and Laz-EcoR I-R. The β-galactosidase gene lacZ, including restriction endonuclease sites such as BamHI, SalI, PstI, and SphI, was amplified and then digested by BglII and EcoRI. It was then cloned into pRSETb plasmid between BamHI and EcoRI sites to generate the pLIC vector.
See Author's Map and published article for more information.
Proper citation: RRID:Addgene_27989 Copy
Species: Mus musculus
Genetic Insert: 5.2 kB VEGF
Vector Backbone Description: Backbone Size:5598; Vector Backbone:PGL2 Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_27987 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7484; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: pTGV-Express contains PBAD and also includes the Shine-Dalgarno prokaryotic translation-initiation sequence for expression of heterologous genes in E. coli.
A single nucleotide deletion was detected in Addgene's quality control sequence compared to the depositing scientist's sequence at bp# 3277. The depositing scientist says this deletion has no impact on the function of the plasmid.
Proper citation: RRID:Addgene_28039 Copy
Species: Homo sapiens
Genetic Insert: SC4MOL
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
References:
Comments: Y244C mutation as described in He et al., J Clin Invest. 2011;121(3):976–984. PMID: 21285510
Proper citation: RRID:Addgene_27985 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:8136; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: A single nucleotide deletion was detected in Addgene's quality control sequence compared to the depositing scientist's sequence at bp# 3929. The depositing scientist says this deletion has no impact on the function of the plasmid.
Proper citation: RRID:Addgene_28037 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7990; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: pTGV-Express contains PBAD and also includes the Shine-Dalgarno prokaryotic translation-initiation sequence for expression of heterologous genes in E. coli.
Proper citation: RRID:Addgene_28038 Copy
Species: Homo sapiens
Genetic Insert: SC4MOL
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_27980 Copy
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