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On page 37 showing 721 ~ 740 out of 739,423 results
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http://www.addgene.org/28066

Species: Danio rerio
Genetic Insert: Zinc finger array targeting Encephalopsin (Opn3)
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Encephalopsin (Opn3)(see below). Please note that this plasmid does NOT contain the Encephalopsin (Opn3) sequence. Users must order the complementary plasmid Encephalopsin (Opn3)_R (OZ542) [Addgene plasmid 28067] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-TGGGATGGA-3' in a bacterial two hybrid assay, and resulted in 8.58 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_28066 Copy   

    • RRID:Addgene_28083

http://www.addgene.org/28083

Species: Danio rerio
Genetic Insert: Zinc finger array targeting Atg7
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Atg7 (see below). Please note that this plasmid does NOT contain the Atg7 sequence. Users must order the complementary plasmid Atg7_L (OZ557) [Addgene plasmid 28082] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GTGGTGGCT-3' in a bacterial two hybrid assay, and resulted in 6.91 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_28083 Copy   

http://www.addgene.org/28080

Species: Danio rerio
Genetic Insert: Zinc finger array targeting cdkn1a(p21)
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene cdkn1a(p21) (see below). Please note that this plasmid does NOT contain the cdkn1a(p21) sequence. Users must order the complementary plasmid cdkn1a(p21)_R (OZ556) [Addgene plasmid 28081] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGTGAACG-3' in a bacterial two hybrid assay, and resulted in 14.32 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_28080 Copy   

http://www.addgene.org/28079

Species: Danio rerio
Genetic Insert: Zinc finger array targeting REST corepressor 2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene REST corepressor 2 (see below). Please note that this plasmid does NOT contain the REST corepressor 2 sequence. Users must order the complementary plasmid REST corepressor 2_L (OZ553) [Addgene plasmid 28078] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-TGCGATGGA-3' in a bacterial two hybrid assay, and resulted in 6.66 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_28079 Copy   

    • RRID:Addgene_28077

http://www.addgene.org/28077

Species: Danio rerio
Genetic Insert: Zinc finger array targeting GUSB
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene GUSB (see below). Please note that this plasmid does NOT contain the GUSB sequence. Users must order the complementary plasmid GUSB_L (OZ551) [Addgene plasmid 28076] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGCGGAC-3' in a bacterial two hybrid assay, and resulted in 11.33 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_28077 Copy   

http://www.addgene.org/28078

Species: Danio rerio
Genetic Insert: Zinc finger array targeting REST corepressor 2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene REST corepressor 2 (see below). Please note that this plasmid does NOT contain the REST corepressor 2 sequence. Users must order the complementary plasmid REST corepressor 2_R (OZ554) [Addgene plasmid 28079] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GATGGTGAG-3' in a bacterial two hybrid assay, and resulted in 10.65 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_28078 Copy   

http://www.addgene.org/28075

Species: Danio rerio
Genetic Insert: Zinc finger array targeting histamine receptor H3 (hrh3)
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene histamine receptor H3 (hrh3) (see below). Please note that this plasmid does NOT contain the histamine receptor H3 (hrh3) sequence. Users must order the complementary plasmid histamine receptor H3 (hrh3)_L (OZ549) [Addgene plasmid 28074] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGACTGC-3' in a bacterial two hybrid assay, and resulted in 12.10 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_28075 Copy   

    • RRID:Addgene_28050

    This resource has 1+ mentions.

http://www.addgene.org/28050

Species: Homo sapiens
Genetic Insert: Rab7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_28050 Copy   

http://www.addgene.org/27999

Species: E. Coli
Genetic Insert: FLIPglu-170nΔ13V
Vector Backbone Description: Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_27999 Copy   

http://www.addgene.org/27998

Species: E. Coli
Genetic Insert: FLIPglu-30μΔ13V
Vector Backbone Description: Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_27998 Copy   

    • RRID:Addgene_27996

    This resource has 1+ mentions.

http://www.addgene.org/27996

Species:
Genetic Insert: TRMPV-Hygro (shLuci-1309)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9339; Vector Backbone:pQCXIX; Vector Types:Mammalian Expression, Retroviral, RNAi, fluorescent reporters; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_27996 Copy   

    • RRID:Addgene_27993

    This resource has 1+ mentions.

http://www.addgene.org/27993

Species:
Genetic Insert: TtTMPV-Neo (shRen)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9003; Vector Backbone:pQCXIX (modified); Vector Types:Mammalian Expression, Retroviral, RNAi, Fluorescent reporters; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_27993 Copy   

    • RRID:Addgene_28048

    This resource has 10+ mentions.

http://www.addgene.org/28048

Species: Homo sapiens
Genetic Insert: Rab7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_28048 Copy   

    • RRID:Addgene_28049

    This resource has 10+ mentions.

http://www.addgene.org/28049

Species: Homo sapiens
Genetic Insert: Rab7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_28049 Copy   

    • RRID:Addgene_27991

    This resource has 1+ mentions.

http://www.addgene.org/27991

Species:
Genetic Insert: TRMPV-ns (shRen)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7722; Vector Backbone:pQCXIX (modified); Vector Types:Mammalian Expression, Retroviral, RNAi, Fluorescent reporters; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_27991 Copy   

    • RRID:Addgene_28047

    This resource has 10+ mentions.

http://www.addgene.org/28047

Species: Homo sapiens
Genetic Insert: Rab7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_28047 Copy   

    • RRID:Addgene_28044

    This resource has 1+ mentions.

http://www.addgene.org/28044

Species: Homo sapiens
Genetic Insert: Rab5A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5078; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_28044 Copy   

    • RRID:Addgene_27990

    This resource has 1+ mentions.

http://www.addgene.org/27990

Species:
Genetic Insert: TRMPV-Neo (shRen)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9117; Vector Backbone:pQCXIX (modified); Vector Types:Mammalian Expression, Retroviral, RNAi, Fluorescent reporters; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_27990 Copy   

    • RRID:Addgene_28045

    This resource has 1+ mentions.

http://www.addgene.org/28045

Species: Homo sapiens
Genetic Insert: Rab5A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_28045 Copy   

    • RRID:Addgene_28062

    This resource has 1+ mentions.

http://www.addgene.org/28062

Species:
Genetic Insert: FokI endonuclease
Vector Backbone Description: Backbone Size:5725; Vector Backbone:pMLM290; Vector Types:Mammalian Expression, Zebrafish expression; Bacterial Resistance:Ampicillin
References:
Comments: For expression of a ZFN targeted to a site with a 5 or 6 bp spacer and “+” KKR heterodimeric FokI domain. (Doyon et. al,Nat Biotech 2011) in mammalian cells or zebrafish as described by Moore et al., PLoS One 2012 (https://www.ncbi.nlm.nih.gov/pubmed/22655075) and Cade et al., Nucleic Acids Res 2012 (https://www.ncbi.nlm.nih.gov/pubmed/22684503)

Proper citation: RRID:Addgene_28062 Copy   

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