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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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Encephalopsin (Opn3)_L (OZ541) Resource Report Resource Website |
RRID:Addgene_28066 | Zinc finger array targeting Encephalopsin (Opn3) | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Encephalopsin (Opn3)(see below). Please note that this plasmid does NOT contain the Encephalopsin (Opn3) sequence. Users must order the complementary plasmid Encephalopsin (Opn3)_R (OZ542) [Addgene plasmid 28067] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-TGGGATGGA-3' in a bacterial two hybrid assay, and resulted in 8.58 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-07-25 12:45:24 | 0 | |
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Atg7_R (OZ558) Resource Report Resource Website |
RRID:Addgene_28083 | Zinc finger array targeting Atg7 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Atg7 (see below). Please note that this plasmid does NOT contain the Atg7 sequence. Users must order the complementary plasmid Atg7_L (OZ557) [Addgene plasmid 28082] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GTGGTGGCT-3' in a bacterial two hybrid assay, and resulted in 6.91 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-07-25 12:45:24 | 0 | |
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cdkn1a(p21)_L (OZ555) Resource Report Resource Website |
RRID:Addgene_28080 | Zinc finger array targeting cdkn1a(p21) | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene cdkn1a(p21) (see below). Please note that this plasmid does NOT contain the cdkn1a(p21) sequence. Users must order the complementary plasmid cdkn1a(p21)_R (OZ556) [Addgene plasmid 28081] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGTGAACG-3' in a bacterial two hybrid assay, and resulted in 14.32 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-07-25 12:45:24 | 0 | |
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REST corepressor 2_R (OZ554) Resource Report Resource Website |
RRID:Addgene_28079 | Zinc finger array targeting REST corepressor 2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene REST corepressor 2 (see below). Please note that this plasmid does NOT contain the REST corepressor 2 sequence. Users must order the complementary plasmid REST corepressor 2_L (OZ553) [Addgene plasmid 28078] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-TGCGATGGA-3' in a bacterial two hybrid assay, and resulted in 6.66 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-07-25 12:45:24 | 0 | |
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GUSB_R (OZ552) Resource Report Resource Website |
RRID:Addgene_28077 | Zinc finger array targeting GUSB | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene GUSB (see below). Please note that this plasmid does NOT contain the GUSB sequence. Users must order the complementary plasmid GUSB_L (OZ551) [Addgene plasmid 28076] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGCGGAC-3' in a bacterial two hybrid assay, and resulted in 11.33 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-07-25 12:45:24 | 0 | |
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REST corepressor 2_L (OZ553) Resource Report Resource Website |
RRID:Addgene_28078 | Zinc finger array targeting REST corepressor 2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene REST corepressor 2 (see below). Please note that this plasmid does NOT contain the REST corepressor 2 sequence. Users must order the complementary plasmid REST corepressor 2_R (OZ554) [Addgene plasmid 28079] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GATGGTGAG-3' in a bacterial two hybrid assay, and resulted in 10.65 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-07-25 12:45:24 | 0 | |
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histamine receptor H3 (hrh3)_R (OZ550) Resource Report Resource Website |
RRID:Addgene_28075 | Zinc finger array targeting histamine receptor H3 (hrh3) | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene histamine receptor H3 (hrh3) (see below). Please note that this plasmid does NOT contain the histamine receptor H3 (hrh3) sequence. Users must order the complementary plasmid histamine receptor H3 (hrh3)_L (OZ549) [Addgene plasmid 28074] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGACTGC-3' in a bacterial two hybrid assay, and resulted in 12.10 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-07-25 12:45:24 | 0 | |
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Flag-Rab7A Q86L Resource Report Resource Website 1+ mentions |
RRID:Addgene_28050 | Rab7A | Homo sapiens | Ampicillin | PMID:20974968 | Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Q86L | 2026-07-25 12:45:24 | 1 | |
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pDR-GW FLIPglu-170nΔ13V Resource Report Resource Website |
RRID:Addgene_27999 | FLIPglu-170nΔ13V | E. Coli | Ampicillin | PMID:20854260 | Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | WT binding protein (no F16A) | 2026-07-25 12:45:24 | 0 | |
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pDR-GW FLIPglu-30μΔ13V Resource Report Resource Website |
RRID:Addgene_27998 | FLIPglu-30μΔ13V | E. Coli | Ampicillin | PMID:20854260 | Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | D154A in mglB | 2026-07-25 12:45:24 | 0 | |
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TRMPV-Hygro Resource Report Resource Website 1+ mentions |
RRID:Addgene_27996 | TRMPV-Hygro (shLuci-1309) | Ampicillin | PMID:21131983 | Backbone Marker:Clontech; Backbone Size:9339; Vector Backbone:pQCXIX; Vector Types:Mammalian Expression, Retroviral, RNAi, fluorescent reporters; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:24 | 1 | |||
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TtTMPV-Neo Resource Report Resource Website 1+ mentions |
RRID:Addgene_27993 | TtTMPV-Neo (shRen) | Ampicillin | PMID:21131983 | Backbone Marker:Clontech; Backbone Size:9003; Vector Backbone:pQCXIX (modified); Vector Types:Mammalian Expression, Retroviral, RNAi, Fluorescent reporters; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:24 | 4 | |||
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EGFP-Rab7A T22N Resource Report Resource Website 10+ mentions |
RRID:Addgene_28048 | Rab7A | Homo sapiens | Ampicillin | PMID:20974968 | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | T22N | 2026-07-25 12:45:24 | 12 | |
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EGFP-Rab7A Q67L Resource Report Resource Website 10+ mentions |
RRID:Addgene_28049 | Rab7A | Homo sapiens | Ampicillin | PMID:20974968 | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Q67L | 2026-07-25 12:45:24 | 19 | |
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TRMPV-ns Resource Report Resource Website 1+ mentions |
RRID:Addgene_27991 | TRMPV-ns (shRen) | Ampicillin | PMID:21131983 | Backbone Marker:Clontech; Backbone Size:7722; Vector Backbone:pQCXIX (modified); Vector Types:Mammalian Expression, Retroviral, RNAi, Fluorescent reporters; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:24 | 1 | |||
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EGFP-Rab7A Resource Report Resource Website 10+ mentions |
RRID:Addgene_28047 | Rab7A | Homo sapiens | Ampicillin | PMID:20974968 | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:24 | 23 | ||
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Myc-Rab5A S34N Resource Report Resource Website 1+ mentions |
RRID:Addgene_28044 | Rab5A | Homo sapiens | Ampicillin | PMID:20974968 | Backbone Marker:Invitrogen; Backbone Size:5078; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S34N | 2026-07-25 12:45:24 | 1 | |
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TRMPV-Neo Resource Report Resource Website 1+ mentions |
RRID:Addgene_27990 | TRMPV-Neo (shRen) | Ampicillin | PMID:21131983 | Backbone Marker:Clontech; Backbone Size:9117; Vector Backbone:pQCXIX (modified); Vector Types:Mammalian Expression, Retroviral, RNAi, Fluorescent reporters; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:24 | 3 | |||
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EGFP-Rab5A S34N Resource Report Resource Website 1+ mentions |
RRID:Addgene_28045 | Rab5A | Homo sapiens | Ampicillin | PMID:20974968 | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S34N | 2026-07-25 12:45:24 | 9 | |
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pCLR2068 Resource Report Resource Website 1+ mentions |
RRID:Addgene_28062 | FokI endonuclease | Ampicillin | PMID:17603475 | For expression of a ZFN targeted to a site with a 5 or 6 bp spacer and “+” KKR heterodimeric FokI domain. (Doyon et. al,Nat Biotech 2011) in mammalian cells or zebrafish as described by Moore et al., PLoS One 2012 (https://www.ncbi.nlm.nih.gov/pubmed/22655075) and Cade et al., Nucleic Acids Res 2012 (https://www.ncbi.nlm.nih.gov/pubmed/22684503) | Backbone Size:5725; Vector Backbone:pMLM290; Vector Types:Mammalian Expression, Zebrafish expression; Bacterial Resistance:Ampicillin | Contains H537R point mutation in FokI domain, as well as E490K and I538K present in the vector backbone | 2026-07-25 12:45:24 | 1 |
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