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Species: Homo sapiens
Genetic Insert: DNMT3A
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_100090 Copy
Species: Homo sapiens
Genetic Insert: SUVAR39H1
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_100088 Copy
Species: Mus musculus
Genetic Insert: Ezh2[SET]
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_100087 Copy
Species: Mus musculus
Genetic Insert: 14056
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_100086 Copy
Species: Homo sapiens
Genetic Insert: FOG1(aa 1-45)
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_100085 Copy
Species: Homo sapiens
Genetic Insert: mCerulean-P2A-putative cMYC DBD with a HA tag
Vector Backbone Description: Backbone Marker:Michel Sadelain (Addgene plasmid # 23244); Vector Backbone:pLM-mCerulean-cMYC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28813671
Proper citation: RRID:Addgene_100125 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dhr1
Vector Backbone Description: Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Comments: Addgene NGS results found S1073N, and an extra 16 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1.
Proper citation: RRID:Addgene_100003 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dhr1
Vector Backbone Description: Vector Backbone:pAS2-1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Comments: Addgene NGS results found S1073N, and an extra 30 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1.
Proper citation: RRID:Addgene_100002 Copy
Species: Homo sapiens
Genetic Insert: G9A
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_100089 Copy
Species: Homo sapiens
Genetic Insert: miR-183-96-82 Cluster promoter 0.8 Kb Fragment
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4322; Vector Backbone:pGL4; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28412746
Comments: Addgene's sequencing results found two discrepancies compared to the depositor's sequence: 123_124insAATT, 162delG, & 854G>T. Some differences could be SNPs and do not occur near the binding sites of the transcription factors of interest; thus they should not affect plasmid function.
Proper citation: RRID:Addgene_100129 Copy
Species: Caenorhabditis elegans
Genetic Insert: zif-1
Vector Backbone Description: Backbone Marker:Christian Frøkjær-Jensen (Erik Jorgensen lab); Backbone Size:7328; Vector Backbone:pCFJ151; Vector Types:Targeting vector for Mos1 transposon mediated single copy transgene insertion; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28619826
Proper citation: RRID:Addgene_100006 Copy
Species: Homo sapiens
Genetic Insert: miR-183-96-82 Cluster promoter 4.8Kb Fragment
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4334; Vector Backbone:pGL4; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28412746
Comments: Addgene's sequencing results found the following discrepancies compared to the depositor's sequence: 367A>G, 739G>A, 928_929insA, 945delA, 1009C>T, 1682G>A, 2324A>T, 2503G>C, 3233A>G, 4092delG, 4784G>T. These differences could be SNPs and do not occur near the binding sites of the transcription factors of interest; thus they should not affect plasmid function.
Proper citation: RRID:Addgene_100127 Copy
Species: Homo sapiens
Genetic Insert: miR-183-96-82 Cluster promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4334; Vector Backbone:pGL4; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28412746
Comments: Addgene's sequencing results found two discrepancies compared to the depositor's sequence: 555delG, 1247G>T. These differences could be SNPs and do not occur near the binding sites of the transcription factors of interest; thus they should not affect plasmid function.
Proper citation: RRID:Addgene_100126 Copy
Species: Homo sapiens
Genetic Insert: STAR protein
Vector Backbone Description: Backbone Size:4000; Vector Backbone:HMBP-3C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26555181
Comments: The STARD1 domain sequence was subcloned after 3C site to obtain 3C cleavable His6-MBP-3C-STAR construction.
Proper citation: RRID:Addgene_100094 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3 sigma human
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28-his-3C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28931924
Comments: The 3C site was introduced immediately prior to 14-3-3 sequence to facilitate purification by subtractive IMAC.
Proper citation: RRID:Addgene_100093 Copy
Species: Homo sapiens
Genetic Insert: Fetal microtubule-associated protein tau
Vector Backbone Description: Backbone Size:5231; Vector Backbone:pET28-his-3C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28575131
Comments: The 3C site is introduced in front of Tau sequence for easy purification by subtractive IMAC
Proper citation: RRID:Addgene_100092 Copy
Species: Homo sapiens
Genetic Insert: DNA fragmentation factor subunit alpha
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26106156
Comments: Please note- insertion of amino acids ENLYFG after AA117, and insertion of ENLYFQSG after AA224 in ICAD [Genbank sequence NP_004392.1.] are expected insertions. They make the ICAD variant recognizable and cleavable by TEVP.
Proper citation: RRID:Addgene_100098 Copy
Species: Homo sapiens
Genetic Insert: Kruppel like factor 4
Vector Backbone Description: Backbone Size:10102; Vector Backbone:pEIGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28412746
Proper citation: RRID:Addgene_100131 Copy
Species: Synthetic
Genetic Insert: ECFP-TevS-YPET
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20723762
Proper citation: RRID:Addgene_100097 Copy
Species: Bacillus alcalophilus
Genetic Insert: NsvBa
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:6200; Vector Backbone:pTracer-CMV2, IRES GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25385530
Proper citation: RRID:Addgene_100015 Copy
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