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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
PB-TO-NGN2-EMX1 puro-BFP
 
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RRID:Addgene_182312 hNGN2-P2A-EMX1 Homo sapiens Ampicillin Backbone Size:13225; Vector Backbone:pUCM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:01:27 0
pLenti GW V5 Eco/Dam hum LaminA
 
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RRID:Addgene_182673 Lamin A Homo sapiens Ampicillin PMID:23124521 Addgene QC NGS analysis returned BLAST results with significant sequence identity to Lamin A/C. Backbone Size:8589; Vector Backbone:pLenti Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 01:01:30 0
pBabepuro-myc-ER
 
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10+ mentions
RRID:Addgene_19128 c-myc Homo sapiens Ampicillin PMID:15367674 Please see Littlewood et al., Nucleic Acids Res. 1995 May 25;23(10):1686-90 for more information regarding the mutant hormone binding domain of the mouse estrogen receptor (hbER Tam). Backbone Size:5169; Vector Backbone:pBabepuro3:hbER tam; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:01:41 16
pBABE Gal4(1-94) HSF ABC Flag ac
 
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RRID:Addgene_1946 Heat Shock Factor 1 Homo sapiens Ampicillin PMID:11486022 contains the FLAG epitope amino terminal to the GAL4 DNA binding domain (amino acids 1 to 94), attached to the C terminus of hHSF1, (amino acids 201 to 529). This construct was subcloned into the pBABE/puro retroviral vector via the BglII and EcoRI sites. Acidic Mutant. (Kingston #1153) Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin acidic mutant (D416, E493, E496 A) 2026-08-29 01:01:44 0
pBMTB-6
 
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RRID:Addgene_22823 Other PMID:16496398 Addgene's QC sequence shows a 1bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity. Backbone Size:7044; Vector Backbone:pBMTB-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2026-08-29 01:02:21 0
pDONR223-HIPK1
 
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RRID:Addgene_23852 HIPK1 Homo sapiens Spectinomycin PMID:21107320 The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately. Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin 2026-08-29 01:02:29 0
pDONR223-ITK
 
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RRID:Addgene_23881 ITK Homo sapiens Spectinomycin PMID:21107320 The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately. Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin 2026-08-29 01:02:27 0
pHAGE-EGFR-A763_Y764insFQEA
 
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RRID:Addgene_116233 EGFR Homo sapiens Ampicillin PMID:29533785 Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin A763_Y764insFQEA 2026-08-29 12:52:28 0
pGLOW63
 
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RRID:Addgene_178877 wrmScarlet11^SEC^3xMyc Caenorhabditis elegans Ampicillin The split-wrmScarlet system was developed and published by Goudeau et al. Genetics 2021. pGLOW63 is a derivative of published TagRFP-SEC vectors (Dickinson et al. Genetics 2015). It has a 3xMyc tag in place of 3xFlag and Lox2272 sites in place of LoxP. These features allow pGLOW63 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites. pGLOW63 must also be used in a genetic background that has wrmScarlet1-10. Made by Amelie Perez (Glow Worms '21). Backbone Size:3881; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin Added ATG start codon to wrmScarlet11 sequence for N-terminal tags 2026-08-29 01:01:10 0
10e8v4 scFv
 
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RRID:Addgene_175264 10e8v4 variable domain Synthetic Kanamycin PMID:33676924 Backbone Marker:Genescript; Vector Backbone:pET26b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin NA 2026-08-29 01:00:36 0
pH6HTN_minus_cer-region
 
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RRID:Addgene_175298 Ampicillin Vector Backbone:pH6HTN; Vector Types:Bacterial Expression, Other, Cell Free Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:00:36 0
pAd5-B6/7
 
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RRID:Addgene_175747 Adenovirus 5 genomic region 25043-35938 Ampicillin PMID:35098167 Backbone Marker:ThermoFisher Scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Adenoviral, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:00:40 0
pACYC-T7CyDisCo
 
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RRID:Addgene_176405 Mitochondrial FAD-linked sulfhydryl oxidase Saccharomyces cerevisiae Chloramphenicol Please visit https://www.biorxiv.org/content/10.1101/2021.08.31.458447v1 for bioRxiv preprint. Backbone Size:5068; Vector Backbone:pACYC184-TU2A-RFP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol Codon optimised for expression in E. coli 2026-08-29 01:00:46 0
pACYC-T7FunCyDisCo
 
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RRID:Addgene_176404 FAD-linked sulfhydryl oxidase ERV2 from Fol Other Chloramphenicol Please visit https://www.biorxiv.org/content/10.1101/2021.08.31.458447v1 for bioRxiv preprint. Backbone Size:5068; Vector Backbone:pACYC184-TU2A-RFP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol deleted amino acids 2-25, codon optimised for E. coli expression 2026-08-29 01:00:46 0
pBABE-hygro
 
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10+ mentions
RRID:Addgene_1765 Ampicillin PMID:2194165 Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication: Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96. If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC. Please note that the sequence from which the Addgene map was generated is an estimate of the real sequence based on how the vector was assembled. We encourage scientists to test enzymes before using them for cloning. Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:00:47 36
pBABE-zeo (pBABE-bleo)
 
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10+ mentions
RRID:Addgene_1766 Ampicillin PMID:2194165 Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication: Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96. SspI site in Amp gene. If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC. Backbone Size:4888; Vector Backbone:pBABE-zeo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:00:48 12
pLenti-Ef1a-mClover-YTHDC1-T2A-BSD-siRNA-Resistant
 
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1+ mentions
RRID:Addgene_177129 YTHDC1 Homo sapiens Ampicillin PMID:34375583 Vector Backbone:modified from Addgene 61425; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin siRNA resistant silent mutations 2026-08-29 01:00:53 1
pBABEpuro GFP-LC3
 
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50+ mentions
RRID:Addgene_22405 microtubule-associated protein 1 light chain 3 beta Rattus norvegicus Ampicillin PMID:18094039 LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. Kabeya Y et al. (EMBO J. 2000 Nov 1. 19(21):5720-8. Backbone Marker:Addgene plasmid # 1764; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:20 98
Flag-HA-OTUD4
 
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RRID:Addgene_22594 OTUD4 Homo sapiens Ampicillin PMID:19615732 Backbone Marker:Harper_Lab; Backbone Size:0; Vector Backbone:pDEST_Tet_ON_CMV_N_FLAG_HA_PGK_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin None 2026-08-29 01:02:23 0
dCas9 plasmid
 
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1+ mentions
RRID:Addgene_100091 Ampicillin PMID:28973434 Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-09-01 09:09:17 7

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