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Species: Synthetic
Genetic Insert: pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
Vector Backbone Description: Vector Backbone:pegRNA entry vector (MNW320); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39039307
Comments: Please visit https://doi.org/10.1101/2023.09.12.557440 for bioRxiv preprint.
Proper citation: RRID:Addgene_208977 Copy
Species: Active sludge microbial communities of municipal wastewater-treating anaerobic digesters from various locations
Genetic Insert: Ga0116245_10002768 candidate type VII proteins
Vector Backbone Description: Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205984 Copy
Species: Active sludge microbial communities of municipal wastewater-treating anaerobic digesters from various locations
Genetic Insert: Ga0116245_10002768 candidate type VII proteins
Vector Backbone Description: Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Comments: R197S frameshift mutation in Cas7 ORF will not affect plasmid function.
Proper citation: RRID:Addgene_205983 Copy
Species: Homo sapiens
Genetic Insert: SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37813990
Comments: Stbl3 is also a suitable growth strain.
Proper citation: RRID:Addgene_210709 Copy
Species: Homo sapiens
Genetic Insert: MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
Vector Backbone Description: Vector Backbone:lenti(AMP); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37813990
Comments: Stbl3 is also a suitable growth strain.
Proper citation: RRID:Addgene_210704 Copy
Species: Homo sapiens
Genetic Insert: H3C2 Homology Arms flanking a mScarlet Tag
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39668248
Comments: To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 https://www.addgene.org/207780 . Please visit https://www.biorxiv.org/content/10.1101/2023.11.01.565029v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_207781 Copy
Species: Homo sapiens
Genetic Insert: LAMP1 Homology Arms flanking a moxGFP-Puro Cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39668248
Comments: To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787): https://www.addgene.org/207787 . Please visit https://www.biorxiv.org/content/10.1101/2023.11.01.565029v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_207788 Copy
Species: Homo sapiens
Genetic Insert: sgRNA Targeting N-terminus of GOLGA2
Vector Backbone Description: Backbone Marker:Addgene #127875; Backbone Size:8947; Vector Backbone:pX330-BbsI-PITCh; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39668248
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.11.01.565029v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_207791 Copy
Species: bacteriophage MS2
Genetic Insert: MS2 loop containing Cj Cas9 gRNA
Vector Backbone Description: Backbone Marker:Genescript; Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37813990
Comments: Stbl3 is also a suitable growth strain.
Proper citation: RRID:Addgene_210711 Copy
Species: Homo sapiens
Genetic Insert: SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37813990
Comments: Stbl3 is also a suitable growth strain.
Proper citation: RRID:Addgene_210710 Copy
Species: Homo sapiens
Genetic Insert: MAPRE1 Homology Arms flanking a moxGFP-Puro Cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39668248
Comments: To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 https://www.addgene.org/207793 . Please visit https://www.biorxiv.org/content/10.1101/2023.11.01.565029v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_207794 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-I36D-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210162 Copy
Species: Photinus pyralis
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Size:6685; Vector Backbone:pRRLSIN.cPPT.MND; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38001268
Comments: Please note: Plasmid contains a V294F mutation in luciferase. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_183502 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::aro7-D147V-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210169 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-I245N-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210166 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210167 Copy
Species: Dictyostelium discoideum
Genetic Insert: PkgE
Vector Backbone Description: Vector Backbone:pDM1209; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37379843
Proper citation: RRID:Addgene_207449 Copy
Genetic Insert: ColE1 Origin of Replication
Vector Backbone Description: Backbone Size:1061; Vector Backbone:Ampicillin Resistance Gene; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37712562
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.06.05.543168v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_203924 Copy
Species: E. coli
Genetic Insert: broad-spectrum class A beta-lactamase TEM-116
Vector Backbone Description: Backbone Size:4743; Vector Backbone:pHI1; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_203880 Copy
Species: E. coli
Genetic Insert: monomeric red fluorescent protein
Vector Backbone Description: Backbone Size:4743; Vector Backbone:pHI1; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_203882 Copy
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