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Species: Gallus gallus
Genetic Insert: pyruvate kinase
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11152524
Proper citation: RRID:Addgene_24163 Copy
Species: Gallus gallus
Genetic Insert: HUR
Vector Backbone Description: Vector Backbone:pH6HTC_minus_cer-region; Vector Types:Bacterial Expression, Cell Free Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.11.08.467743 for bioRxiv preprint.
Proper citation: RRID:Addgene_175313 Copy
Species: Gallus gallus
Genetic Insert: TUBB3
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:cDNA for in situ hybridization; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12486167
Comments: For riboprobes, cut with EagI. Runoffs with T7.
Proper citation: RRID:Addgene_31232 Copy
Species: Gallus gallus
Genetic Insert: SHH
Vector Backbone Description: Backbone Marker:A. D. Johnson, P. A. Krieg, Dev. Genet. 17, 280 (1995); Vector Backbone:pXeX; Vector Types:Xenopus expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11251119
Proper citation: RRID:Addgene_31233 Copy
Species: Gallus gallus
Genetic Insert: DISP1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII-TOPO; Vector Types:cDNA for in situ hybridization; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:16246323
Comments: For riboprobe cut with SpeI. Runoffs with T7 polymerase. This is not full length ORF.
Proper citation: RRID:Addgene_31235 Copy
Species: Gallus gallus
Genetic Insert: OVA
Vector Backbone Description: Backbone Marker:Reff et al., 1994; Backbone Size:9209; Vector Backbone:TCAE (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9364701
Comments: *The backbone (TCAE) was modified to eliminate the immunoglobulin variable and constant regions, the neomycin resistance gene, and the DHFR gene. See paper for more details.
Proper citation: RRID:Addgene_31598 Copy
Species: Gallus gallus
Genetic Insert: 1XDR4
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Comments: Note that there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences should not affect plasmid function.
Proper citation: RRID:Addgene_32614 Copy
Species: Gallus gallus
Genetic Insert: Thyroid Response Element
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32612 Copy
Species: Gallus gallus
Genetic Insert: rGH-PAL
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32617 Copy
Species: Gallus gallus
Genetic Insert: GAPDH shRNA #2
Vector Backbone Description: Vector Backbone:pRFP RNAiC-1; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32622 Copy
Species: Gallus gallus
Genetic Insert: TRbeta shRNA #1
Vector Backbone Description: Vector Backbone:pRFP RNAiC-1; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32620 Copy
Species: Gallus gallus
Genetic Insert: ASIC1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_32791 Copy
Species: Gallus gallus
Genetic Insert: shh
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(E); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8269518
Proper citation: RRID:Addgene_13992 Copy
Species: Gallus gallus
Genetic Insert: shh
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8269518
Proper citation: RRID:Addgene_13991 Copy
Species: Gallus gallus
Genetic Insert: Hoxd-13
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9043077
Comments: RCASHoxd-13 was constructed using a cDNA corresponding to the longer of the two transcripts whose putative coding region is identical to the published sequence, with the exception of 9 glycines encoded by ggc repeats starting at amino acid position 10, instead of the reported 7 ggc repeats (Rogina and Upholdt, 1993). This cDNA was cloned into the shuttle vector SLAX-13 (Riddle et al., 1993), and then subcloned into the ClaI site of RCAS(BP)A .
Proper citation: RRID:Addgene_13985 Copy
Species: Gallus gallus
Genetic Insert: Hoxc6
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8812121
Comments: To generate a retrovirus capable of transcribing Hoxc-6 mRNA, the region encoding the PR11 Hoxc-6 protein was isolated from a chick cDNA clone (Nelson et al., 1996). The PR11 Hoxc-6 protein comprises 235 amino acids (Oliver et al., 1988; Coletta et al., 1991; Shimeld et al., 1993). The ATG of the PR11 coding region was engineered by PCR for cloning into the NcoI site of the SLAX-13 shuttle vector (Riddle et al., 1993), and then subcloned into the retroviral vector RCAS-BP(A) (Hughes et al., 1987). The insert is 932 base pairs and includes approximately 230 bp of 3' untranslated sequence.
Proper citation: RRID:Addgene_13986 Copy
Species: Gallus gallus
Genetic Insert: Slug
Vector Backbone Description: Backbone Size:3000; Vector Backbone:Slax13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full length coding sequence, PCR (vent) product, 5’ primer converts 2nd codon from cca (pro) to gca (ala) in order to generate a Nco I site; 3’ primer is wt plus EcoRI site, from Malcolm Logan.
Proper citation: RRID:Addgene_14015 Copy
Species: Gallus gallus
Genetic Insert: HoxD10
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full-length Hox D10, from Craig Nelson.
Proper citation: RRID:Addgene_14010 Copy
Species: Gallus gallus
Genetic Insert: Lmx1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For 3'UTR probe cut with Xba1 and use T7 RNA pol, from Bob Riddle.
Proper citation: RRID:Addgene_14024 Copy
Species: Gallus gallus
Genetic Insert: Chicken Beta 1 Integrin
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3487386
Proper citation: RRID:Addgene_14066 Copy
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