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Genetic Insert: tetA
Vector Backbone Description: Backbone Size:8754; Vector Backbone:pMJH46; Vector Types:Bacterial Expression, Red Recombinase, temperature sensitive; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:28352570
Comments: Conjugally transferable red recombinase vector pMJH65
Proper citation: RRID:Addgene_67273 Copy
Genetic Insert: tetA(C)
Vector Backbone Description: Backbone Marker:Alexeyev, M.F., BioTechniques 18:52-56, 1995; Vector Backbone:pBSL15; Vector Types:Bacterial vector based on pMB1 replicon; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:26921431
Proper citation: RRID:Addgene_74110 Copy
Species: Synthetic
Genetic Insert: tetA(C)
Vector Backbone Description: Vector Backbone:pUCBM21; Vector Types:Conjugative bacterial vector; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:26921431
Proper citation: RRID:Addgene_74104 Copy
Genetic Insert: Flp recombinase
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pFLP1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:15908923
Comments: GenBank Accession # AY597273
Compared to the Genbank reference sequence AY597273, Addgene's sequencing results found a T350I mutation in the tetracycline cassette and a G5D mutation in FLP recombinase. The effect of these differences is unknown.
Proper citation: RRID:Addgene_64946 Copy
Genetic Insert: FRT-TcR-FRT
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pFRT2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:15908923
Comments: GenBank Accession #AY712950
Compared to the Genbank reference sequence AY712950, Addgene's sequencing results found a T350I mutation in the tetracycline cassette. The effect of this difference is unknown.
Proper citation: RRID:Addgene_64951 Copy
Species: Caenorhabditis elegans
Genetic Insert: let-363 cDNA
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:22278922
Comments: This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain.
Proper citation: RRID:Addgene_42600 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-15
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:22278922
Comments: This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain.
Note: Addgene's quality control sequencing has found that the deposited clone contains a T to C mismatch with respect to the C. elegans N2 reference daf-15 sequence, which would cause a V797A change in the coded protein. According to the depositing lab, this point mutation does not affect RNAi feeding; however, its possible effects in other contexts are not known.
Proper citation: RRID:Addgene_42601 Copy
Species: Homo sapiens
Genetic Insert: Bystin
Vector Backbone Description: Backbone Size:4033; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:9560222
Proper citation: RRID:Addgene_11869 Copy
Species: Synthetic
Genetic Insert: GFP+
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5796; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology, E. coli and Bacillus shuttle vector; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:29666238
Proper citation: RRID:Addgene_107582 Copy
Species: Synthetic
Genetic Insert: GFP+
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:6336; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:29666238
Proper citation: RRID:Addgene_107578 Copy
Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8387644
Comments: This is Gq alpha with an HA epitope engineered into an internal site that does not seem to affect receptor coupling in multiple studies. Epitope tagged by Paul Wilson, see Wedegaertner, JBC 268: 25001-25008. The 5' non-coding sequences were removed, but the 3' non-coding sequences remain, as in Strathmann & Simon (1990) PNAS 87:9113-9117. The parent construct has been donated to the ATCC by the Bourne Lab.
There is a 1bp insertion, a 5bp insertion, and a 1bp deletion when author sequence is aligned with Addgene quality control sequence. These differences are between the stop codon and the SP6 sequence.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_24502 Copy
Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8387644
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gz alpha residues (EYNLV to YIGLC). Works the same as qi5 and is the least popular since no one knows what Gz alpha really does in nature. Since qz5 is not sensitive to pertussis toxin, qz5 may be the only G protein activated by a Gi-coupled receptor in cells treated with pertussis toxin. This trick can be experimentally useful in settings where you want experimental control of the exact G protein and the receptor that is activated. It is theoretically possible that this construct will work better than the other constructs for particular receptors, but the Conklin lab has not seen this happen yet.
Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_25867 Copy
Species: Homo sapiens
Genetic Insert: RAP1
Vector Backbone Description: Backbone Marker:Anumanthan et al. 1998; Vector Backbone:pAXEF-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:15469846
Proper citation: RRID:Addgene_32697 Copy
Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:1547942
Comments: Addgene sequencing results show that only half of the leurin zipper is deleted, protein ends at amino acid 133.
Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence.
Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.
Proper citation: RRID:Addgene_21900 Copy
Species: Homo sapiens
Genetic Insert: integrin alpha 9 / alpha 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:11598204
Comments: See article for cloning details.
Proper citation: RRID:Addgene_13588 Copy
Species: Homo sapiens
Genetic Insert: integrin alpha 9
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:7523411
Comments: Please note that the first 41 amino acids of the integrin alpha insert show greater identity to integrin alpha-5 rather than integrin alpha-9
Proper citation: RRID:Addgene_13585 Copy
Species: Homo sapiens
Genetic Insert: integrin beta 6 777T
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:12634787
Proper citation: RRID:Addgene_13582 Copy
Species: Synthetic
Genetic Insert: T7A1, O2, O1, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:28295806
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170477 Copy
Species: Synthetic
Genetic Insert: T7A1, O2, Os, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170478 Copy
Species: Synthetic
Genetic Insert: T7A1, O-, O1, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170473 Copy
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