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Vector Backbone Description: Backbone Marker:Kaniga, et al Gene. 1991 109: 137-141.; Backbone Size:8509; Vector Backbone:pKNG101; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin and Chloramphenicol
Defining Citation: PMID:19843225
Comments: Please note that there are several discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_71298 Copy
Species: Other
Genetic Insert: dGFP
Vector Backbone Description: Backbone Marker:Patrick Salmon; Backbone Size:5000; Vector Backbone:pCLX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25732825
Comments: Bisei Ohkawara recloned the CHOP promoter for me after a mix-up with the original plasmids. I received the good plasmids from Japan in June 2014.
from:
Division of Neurogenetics,
Center for Neurological Diseases and Cancer,
Nagoya University Graduate School of Medicine
Institute for advanced research Nagoya University (IAR)
65 Tsurumai, Showa-ku, Nagoya 466-8550, Japan.
Proper citation: RRID:Addgene_71299 Copy
Species: changed Cysteine 176 to Alanine
Genetic Insert: HP0518
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET28(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26306031
Proper citation: RRID:Addgene_71360 Copy
Species: Other
Genetic Insert: NLS-2xMCP-2xTagRFP-T
Vector Backbone Description: Vector Backbone:pHsp83; Vector Types:Insect Expression, P element-mediated germline transformation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25792328
Proper citation: RRID:Addgene_71242 Copy
Species: Homo sapiens
Genetic Insert: cortactin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24469447
Comments: silent mutation present at 72nd nucleotide
Proper citation: RRID:Addgene_71365 Copy
Species: Mus musculus
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24469447
Proper citation: RRID:Addgene_71366 Copy
Vector Backbone Description: Backbone Marker:Nordeen, 1988 and Promega; Vector Backbone:pXP1 and pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10964627
Comments: Depositor states "A spontaneous single C to A mutation was identified 564 bp upstream of the origin of replication at plasmid position 4660 in pXPM which generated high copy replication. A HindIII/XbaI fragment of pGL3 (Promega) containing the improved luciferase gene Luc+ (Sherf et al.,1994) was cloned into into HindIII/XbaI-cut pXPM."
JM109 are the cells used by the author for pXPG and its derivatives.
Proper citation: RRID:Addgene_71248 Copy
Genetic Insert: HP0518
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET28(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26306031
Comments: Please Note: the HP0518 insert has a C-terminal His tag.
Proper citation: RRID:Addgene_71353 Copy
Genetic Insert: HP0518
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET28(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26306031
Proper citation: RRID:Addgene_71351 Copy
Species: changed Tyrosine 133 to Alanine
Genetic Insert: HP0518
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET28(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26306031
Proper citation: RRID:Addgene_71356 Copy
Species: changed Histidine 155 to Alanine
Genetic Insert: HP0518
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET28(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26306031
Proper citation: RRID:Addgene_71357 Copy
Species: changed Tyrosine 132 to Alanine
Genetic Insert: HP0518
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET28(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26306031
Proper citation: RRID:Addgene_71355 Copy
Species: S. Pyogenes
Genetic Insert: humanized dCas9-KRAB T2A GFP
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26501517
Comments: Note: DNA prep concentrations may be low due to the size of this plasmid, so working with low copy protocols, higher starting volumes, or fresh transformants is recommended.
Proper citation: RRID:Addgene_71237 Copy
Species: changed Tryptophan 158 to Alanine
Genetic Insert: HP0518
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET28(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26306031
Proper citation: RRID:Addgene_71358 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RMP1
Vector Backbone Description: Backbone Marker:D. Amberg (State University of New York Upstate Medical University, Syracuse, NY; Vector Backbone:pTD125; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16585272
Comments: Plasmid is also called pTD125 GFP:RMP1
Vector is URA, CEN-based.
Construct has: ACT1promoter-GFP-RMP1-ACT1terminator
Proper citation: RRID:Addgene_71386 Copy
Species: Homo sapiens
Genetic Insert: human synapsin promoter-flex switch-dsRed-shscramble
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26094607
Comments: this plasmid is modified from the pRIME system. See:
Stegmeier, F., Hu, G., Rickles, R.J., Hannon, G.J., and Elledge, S.J. (2005). A
lentiviral microRNA-based system for single-copy polymerase II-regulated
RNA interference in mammalian cells. Proc. Natl. Acad. Sci. USA 102,
13212–13217
We used The pPRIME-CMV-dsRed-FF3 vector, a gift from Stephen Elledge (Addgene plasmid 11664), as the building block to make the AAV-hsyn-flex-dsRed-shscramble plasmid
All the enzymes are unique cutters, except EcoRI and XhoI, the insert dsRed-shRNA can be retrieved using AscI and NheI, giving 1.2kb and 4.8kb bands.
The insert was read using primers in the dsred sequence:
dsred 410: CGTAATGCAGAAGAAGACTATG
dsred 530R: CCATGTAGATGGACTTGAACTC
Proper citation: RRID:Addgene_71383 Copy
Species: Synthetic
Genetic Insert: CMV promoter-dsRed-mir30-shscramble
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26094607
Comments: this plasmid is modified from the pRIME system. See:
Stegmeier, F., Hu, G., Rickles, R.J., Hannon, G.J., and Elledge, S.J. (2005). A
lentiviral microRNA-based system for single-copy polymerase II-regulated
RNA interference in mammalian cells. Proc. Natl. Acad. Sci. USA 102,
13212–13217
We used The pPRIME-CMV-dsRed-FF3 vector, a gift from Stephen Elledge (Addgene plasmid 11664) to make pPRIME-dsRed-scramble
Proper citation: RRID:Addgene_71384 Copy
Species: Drosophila melanogaster
Genetic Insert: Copia
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4526; Vector Backbone:pCoHygro; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71389 Copy
Species: Homo sapiens
Genetic Insert: GIV/Girdin
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71544 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: POP1
Vector Backbone Description: Backbone Marker:D. Amberg (State University of New York Upstate Medical University, Syracuse, NY; Vector Backbone:pTD125; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16585272
Comments: Vector is URA, CEN-based.
Construct has: ACT1promoter-GFP-POP1-ACT1terminator
Proper citation: RRID:Addgene_71387 Copy
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