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Species: Homo sapiens
Genetic Insert: protein kinase R
Vector Backbone Description: Vector Backbone:pet11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42934 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-scrt2-left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
References:
Comments: Target binding site: TTTTCTGGTGCGTCTGTG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42815 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-scrt2-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
References:
Comments: Target binding site: TCACCAGAAACGAGCGAG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42816 Copy
Species: Homo sapiens
Genetic Insert: NKX3.1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET-30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: This is a full length NKX3.1 cDNA construct. Protein expressed in E.coli BL21(DE3) competent cells
Proper citation: RRID:Addgene_42896 Copy
Species: Strongyloides stercoralis
Genetic Insert: attR2::Ss-era-1 terminator (598 bp)::attL3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4773; Vector Backbone:pDONR P2R-P3; Vector Types:Multi-site Gateway donor plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_42928 Copy
Species: Perch (Morone americana)
Genetic Insert: Connexin 35
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4990; Vector Backbone:pcDNA3.1 Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Subcloned from cDNA clone from: O'Brien, J., Bruzzone, R., White, T.W., Al-Ubaidi, M.R., and Ripps, H. (1998). Cloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin family. J Neurosci 18, 7625-7637.
Proper citation: RRID:Addgene_42920 Copy
Species: A. victoria
Genetic Insert: Aquamarine
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_42888 Copy
Species: Perch (Morone americana)
Genetic Insert: Connexin 34.7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4990; Vector Backbone:pcDNA3.1 Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Subcloned from cDNA clone from: O'Brien, J., Bruzzone, R., White, T.W., Al-Ubaidi, M.R., and Ripps, H. (1998). Cloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin family. J Neurosci 18, 7625-7637.
Proper citation: RRID:Addgene_42921 Copy
Species: A. victoria
Genetic Insert: Aquamarine
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4751; Vector Backbone:pProEX HTa; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42889 Copy
Species: Perch (Morone americana)
Genetic Insert: Connexin 35
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4990; Vector Backbone:pcDNA3.1 Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Contains a non-phosphorylatable point mutation in Ser110 in cytoplasmic loop. Phosphorylation of this site regulates coupling.
Derived from clone from: O'Brien, J., Nguyen, H.B., and Mills, S.L. (2004). Cone photoreceptors in bass retina use two connexins to mediate electrical coupling. J Neurosci 24, 5632-5642.
Original cDNA clone from: O'Brien, J., Bruzzone, R., White, T.W., Al-Ubaidi, M.R., and Ripps, H. (1998). Cloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin family. J Neurosci 18, 7625-7637.
Proper citation: RRID:Addgene_42917 Copy
Species:
Genetic Insert: CRISPR-BsaI
Vector Backbone Description: Backbone Size:2254; Vector Backbone:pZE21-MCS1; Vector Types:Bacterial Expression, CRISPR, E.coli; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid is also known as pDB129.
This plasmid contains a duplicated spacer insertion site (4 BsaI sites instead of 2). Please note that these extra sites are eliminated upon treatment with BsaI, and the depositing lab has used this version extensively without issue. Addgene has found that versions of the plasmid with and without the duplicated spacer are present, both are predicted to perform similarly.
The HME63 strain is available from the from the Court Lab:
http://redrecombineering.ncifcrf.gov/strains.html
For more information on Marraffini Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/marraffini/
Proper citation: RRID:Addgene_42875 Copy
Species: Perch (Morone americana)
Genetic Insert: Connexin 35 C-terminal domain
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5930; Vector Backbone:pET42c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Stop codon at CamKII phosphorylation site near tip of the C-terminus. Truncates last 7 amino acids.
Proper citation: RRID:Addgene_42913 Copy
Species: Rattus norvegicus
Genetic Insert: Red Fluorescent Calcium binding Protein
Vector Backbone Description: Backbone Marker:Life Technologies (Invitrogen); Backbone Size:2753; Vector Backbone:pRSETA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Gene was cloned using BaMHI and HindIII - But part of the vector backbone then becomes part of the protein (leader sequence containing histag) - using NdeI and HindIII will completely remove the open reading frame/gene.
Proper citation: RRID:Addgene_42874 Copy
Species:
Genetic Insert: L7.5.1
Vector Backbone Description: Backbone Size:6200; Vector Backbone:pCT; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42900 Copy
Species: Mus musculus
Genetic Insert: PECAM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Compared to GenBank accession number L06039.1, the PECAM-1 sequence in this plasmid contains 2 nucleotide mismatches in the 5' untranslated region (UTR).
Proper citation: RRID:Addgene_42901 Copy
Species: Homo sapiens
Genetic Insert: PECAM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_42902 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-ywhai-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
References:
Comments: Target binding site: TGCTGGACACTACCCTCC
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42860 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-zmynd11-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32290; Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
References:
Comments: Target binding site: TGTTGGCGATTTGTTTCT
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42862 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-Vangl1-left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
References:
Comments: Target binding site: TTCTCTGCCCAGGATAG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42853 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-Vangl1-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
References:
Comments: Target binding site: TGTCAGTAAACCCAGC
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42854 Copy
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