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Plasmids are provided by Addgene and DGRC.

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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pPET-PKR/PPase
 
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RRID:Addgene_42934 protein kinase R Homo sapiens Ampicillin PMID:15567412 Vector Backbone:pet11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:47:06 5
TAL3352
 
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RRID:Addgene_42815 ZebrafishCommunity-scrt2-left Danio rerio Ampicillin PMID:21822241 Target binding site: TTTTCTGGTGCGTCTGTG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-07-25 12:47:05 0
TAL3353
 
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RRID:Addgene_42816 ZebrafishCommunity-scrt2-right Danio rerio Ampicillin PMID:21822241 Target binding site: TCACCAGAAACGAGCGAG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-07-25 12:47:05 0
His-NKX3.1
 
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RRID:Addgene_42896 NKX3.1 Homo sapiens Kanamycin PMID:19780584 This is a full length NKX3.1 cDNA construct. Protein expressed in E.coli BL21(DE3) competent cells Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET-30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-07-25 12:47:05 0
pPV475
 
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RRID:Addgene_42928 attR2::Ss-era-1 terminator (598 bp)::attL3 Strongyloides stercoralis Kanamycin Backbone Marker:Invitrogen; Backbone Size:4773; Vector Backbone:pDONR P2R-P3; Vector Types:Multi-site Gateway donor plasmid; Bacterial Resistance:Kanamycin 2026-07-25 12:47:06 0
PCx35-pcDNA
 
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RRID:Addgene_42920 Connexin 35 Perch (Morone americana) Ampicillin PMID:15201336 Subcloned from cDNA clone from: O'Brien, J., Bruzzone, R., White, T.W., Al-Ubaidi, M.R., and Ripps, H. (1998). Cloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin family. J Neurosci 18, 7625-7637. Backbone Marker:Invitrogen; Backbone Size:4990; Vector Backbone:pcDNA3.1 Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:47:06 1
pAquaN1
 
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RRID:Addgene_42888 Aquamarine A. victoria Kanamycin PMID:23192565 Backbone Marker:Clontech; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T66S and H149G 2026-07-25 12:47:05 3
PCx34.7-pcDNA
 
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RRID:Addgene_42921 Connexin 34.7 Perch (Morone americana) Ampicillin PMID:15201336 Subcloned from cDNA clone from: O'Brien, J., Bruzzone, R., White, T.W., Al-Ubaidi, M.R., and Ripps, H. (1998). Cloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin family. J Neurosci 18, 7625-7637. Backbone Marker:Invitrogen; Backbone Size:4990; Vector Backbone:pcDNA3.1 Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:47:06 0
pPROEX Aqua
 
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RRID:Addgene_42889 Aquamarine A. victoria Ampicillin PMID:23192565 Backbone Marker:Life Technologies; Backbone Size:4751; Vector Backbone:pProEX HTa; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T65S and H148G 2026-07-25 12:47:05 3
PCx35-pcDNA-S110A
 
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RRID:Addgene_42917 Connexin 35 Perch (Morone americana) Ampicillin PMID:15857663 Contains a non-phosphorylatable point mutation in Ser110 in cytoplasmic loop. Phosphorylation of this site regulates coupling. Derived from clone from: O'Brien, J., Nguyen, H.B., and Mills, S.L. (2004). Cone photoreceptors in bass retina use two connexins to mediate electrical coupling. J Neurosci 24, 5632-5642. Original cDNA clone from: O'Brien, J., Bruzzone, R., White, T.W., Al-Ubaidi, M.R., and Ripps, H. (1998). Cloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin family. J Neurosci 18, 7625-7637. Backbone Marker:Invitrogen; Backbone Size:4990; Vector Backbone:pcDNA3.1 Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Ser110 to Ala 2026-07-25 12:47:05 0
pCRISPR
 
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10+ mentions
RRID:Addgene_42875 CRISPR-BsaI Kanamycin PMID:23360965 This plasmid is also known as pDB129. This plasmid contains a duplicated spacer insertion site (4 BsaI sites instead of 2). Please note that these extra sites are eliminated upon treatment with BsaI, and the depositing lab has used this version extensively without issue. Addgene has found that versions of the plasmid with and without the duplicated spacer are present, both are predicted to perform similarly. The HME63 strain is available from the from the Court Lab: http://redrecombineering.ncifcrf.gov/strains.html For more information on Marraffini Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/marraffini/ Backbone Size:2254; Vector Backbone:pZE21-MCS1; Vector Types:Bacterial Expression, CRISPR, E.coli; Bacterial Resistance:Kanamycin 2026-07-25 12:47:05 31
PCx35CT-S298ter
 
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RRID:Addgene_42913 Connexin 35 C-terminal domain Perch (Morone americana) Kanamycin PMID:15857663 Stop codon at CamKII phosphorylation site near tip of the C-terminus. Truncates last 7 amino acids. Backbone Marker:Novagen; Backbone Size:5930; Vector Backbone:pET42c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Ser298 stop codon 2026-07-25 12:47:05 0
pRSET-RCaMP1h
 
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1+ mentions
RRID:Addgene_42874 Red Fluorescent Calcium binding Protein Rattus norvegicus Ampicillin PMID:23459413 Gene was cloned using BaMHI and HindIII - But part of the vector backbone then becomes part of the protein (leader sequence containing histag) - using NdeI and HindIII will completely remove the open reading frame/gene. Backbone Marker:Life Technologies (Invitrogen); Backbone Size:2753; Vector Backbone:pRSETA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:47:05 7
pCT-L7.5.1
 
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1+ mentions
RRID:Addgene_42900 L7.5.1 Ampicillin PMID:18602401 Backbone Size:6200; Vector Backbone:pCT; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:47:05 3
pcDNAI Neo mouse PECAM FL
 
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1+ mentions
RRID:Addgene_42901 PECAM Mus musculus Kanamycin Compared to GenBank accession number L06039.1, the PECAM-1 sequence in this plasmid contains 2 nucleotide mismatches in the 5' untranslated region (UTR). Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-07-25 12:47:05 1
pcDNAI Neo human PECAM FL
 
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RRID:Addgene_42902 PECAM Homo sapiens Kanamycin Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-07-25 12:47:05 1
TAL3397
 
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RRID:Addgene_42860 ZebrafishCommunity-ywhai-right Danio rerio Ampicillin PMID:21822241 Target binding site: TGCTGGACACTACCCTCC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-07-25 12:47:05 0
TAL3399
 
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RRID:Addgene_42862 ZebrafishCommunity-zmynd11-right Danio rerio Ampicillin PMID:21822241 Target binding site: TGTTGGCGATTTGTTTCT It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32290; Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-07-25 12:47:05 0
TAL3390
 
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RRID:Addgene_42853 ZebrafishCommunity-Vangl1-left Danio rerio Ampicillin PMID:21822241 Target binding site: TTCTCTGCCCAGGATAG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-07-25 12:47:05 0
TAL3391
 
Resource Report
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RRID:Addgene_42854 ZebrafishCommunity-Vangl1-right Danio rerio Ampicillin PMID:21822241 Target binding site: TGTCAGTAAACCCAGC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-07-25 12:47:05 0

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