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Species: virus
Genetic Insert: BBLF2/3
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4184; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8970953
Proper citation: RRID:Addgene_72633 Copy
Species: virus
Genetic Insert: BALF5
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4184; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8970953
Proper citation: RRID:Addgene_72630 Copy
Species: virus
Genetic Insert: BMLF1/BSLF2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4184; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8970953
Proper citation: RRID:Addgene_72636 Copy
Species: virus
Genetic Insert: BRLF1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4184; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8970953
Proper citation: RRID:Addgene_72635 Copy
Species: Mus musculus
Genetic Insert: UAS upstream of PSD95-FingR with ZFC(CCR5TC) and KRAB(A)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR 221; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Comments: Details of cloning are in:
Son et al., Scientific Reports, 2016. Transgenic FingRs for Live Mapping of Synaptic Dynamics in Genetically-Defined Neurons.
Proper citation: RRID:Addgene_72638 Copy
Species: Mus musculus
Genetic Insert: UAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR 221; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Comments: PLEASE NOTE- this plasmid has a Stop codon after mCherry which is an error and will cause the construct to not be specifically targeted to the synapse.
The Bonkowsky Lab has a corrected plasmid available which will soon be available at Addgene: pTol2-ziUAS:GPHN.FingR-mKate2-ZFI (IL2RGTC)-KRAB(A).
Details of cloning are in:
Son et al., Scientific Reports, 2016. Transgenic FingRs for Live Mapping of Synaptic Dynamics in Genetically-Defined Neurons.
Proper citation: RRID:Addgene_72639 Copy
Species: Homo sapiens
Genetic Insert: dysferlin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22110769
Proper citation: RRID:Addgene_72585 Copy
Species: Homo sapiens
Genetic Insert: dysferlin C2A domain
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22110769
Proper citation: RRID:Addgene_72582 Copy
Species: Homo sapiens
Genetic Insert: dysferlin C2B domain
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22110769
Proper citation: RRID:Addgene_72583 Copy
Species: Homo sapiens
Genetic Insert: Cep123
Vector Backbone Description: Backbone Marker:Modified Clontech vector; Backbone Size:5781; Vector Backbone:pLP-mEos2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21976302
Proper citation: RRID:Addgene_72589 Copy
Species: Homo sapiens
Genetic Insert: dysferlin
Vector Backbone Description: Vector Backbone:pCMV-9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22110769
Proper citation: RRID:Addgene_72587 Copy
Species: Homo sapiens
Genetic Insert: gRNAs toward Malat1
Vector Backbone Description: Vector Backbone:Lentiguide-puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26493208
Proper citation: RRID:Addgene_72620 Copy
Species: Homo sapiens
Genetic Insert: gRNAs toward Malat1
Vector Backbone Description: Vector Backbone:Lentiguide-puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26493208
Proper citation: RRID:Addgene_72623 Copy
Species: Homo sapiens
Genetic Insert: gRNAs toward Malat1
Vector Backbone Description: Vector Backbone:Lentiguide-puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26493208
Proper citation: RRID:Addgene_72624 Copy
Species: virus
Genetic Insert: BALF2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4184; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8970953
Proper citation: RRID:Addgene_72629 Copy
Species: Homo sapiens
Genetic Insert: gRNAs toward UCA1
Vector Backbone Description: Vector Backbone:Lentiguide-puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26493208
Proper citation: RRID:Addgene_72628 Copy
Genetic Insert: NLS-mCherry-LEXY
Vector Backbone Description: Vector Backbone:pcDNA3.1 (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26853913
Proper citation: RRID:Addgene_72655 Copy
Species: B. subtilis
Genetic Insert: mEos2-MTSBs
Vector Backbone Description: Backbone Marker:NBRP-E.coli at NIG; Backbone Size:4500; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25848771
Proper citation: RRID:Addgene_72652 Copy
Genetic Insert: H2B-GFP-LEXY
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26853913
Proper citation: RRID:Addgene_72658 Copy
Genetic Insert: H2B-GFP-AsLOV2-NES27
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5585; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26853913
Proper citation: RRID:Addgene_72659 Copy
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