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Species: Acetobacter aceti 1023
Genetic Insert: peptidase S9
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72405 Copy
Species: Acetobacter aceti 1023
Genetic Insert: lipase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72406 Copy
Species: Acetobacter aceti 1023
Genetic Insert: succinyl-CoA:acetate CoA-transferase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72403 Copy
Species: Acetobacter aceti 1023
Genetic Insert: feruloyl esterase
Vector Backbone Description: Backbone Size:3663; Vector Backbone:pET23d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72409 Copy
Species: Acetobacter aceti 1023
Genetic Insert: 4-hydroxybenzoyl-CoA thioesterase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72408 Copy
Species: Acetobacter aceti 1023
Genetic Insert: phosphoribosylpyrophosphate synthetase
Vector Backbone Description: Backbone Size:3663; Vector Backbone:pET23d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72390 Copy
Species: Escherichia coli str. K-12 substr. MG1655
Genetic Insert: phosphoribosylaminoimidazole synthetase
Vector Backbone Description: Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Source plasmid contained a Gly324>Asp mutation that we fixed using QuikChange.
Reference for source plasmid:
Schultheisz, H. L., Szymczyna, B. R., Scott, L. G., and Williamson, J. R. (2008) Pathway engineered enzymatic de novo purine nucleotide synthesis ACS Chem. Biol. 3 499 511 [DOI: 10.1021/cb800066p]
Proper citation: RRID:Addgene_72397 Copy
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72318 Copy
Species: Synthetic
Genetic Insert: modified YFP
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72316 Copy
Species: Escherichia coli str. K-12 substr. MG1655
Genetic Insert: cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72382 Copy
Species: Bacillus subtilis
Genetic Insert: phosphoribosylglycinamide synthetase
Vector Backbone Description: Backbone Size:3663; Vector Backbone:pET23d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72383 Copy
Species: Escherichia coli BL21(DE3)
Genetic Insert: phosphoribosylamine-glycine ligase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72386 Copy
Species: Acetobacter aceti 1023
Genetic Insert: lipase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72421 Copy
Species: Acetobacter aceti 1023
Genetic Insert: phosphoribosylpyrophosphate synthetase
Vector Backbone Description: Backbone Size:3663; Vector Backbone:pET23d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72389 Copy
Species: Acetobacter aceti 1023
Genetic Insert: putative CoA-transferase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72425 Copy
Species: Synthetic
Genetic Insert: modified YFP
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72308 Copy
Species: Sindbis virus
Genetic Insert: DH-BB(5'SIN;TE12ORF)
Vector Backbone Description: Vector Backbone:pSIN; Vector Types:Sindbis virus helper construct; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72309 Copy
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26315440
Comments: This is an ancestor strain of CY008. The chloramphenicol resistance marker can be removed by Flp-FRT recombination to obtain CY008.
Proper citation: RRID:Addgene_72337 Copy
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72320 Copy
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72321 Copy
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