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Vector Backbone Description: Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128038 Copy
Vector Backbone Description: Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128037 Copy
Genetic Insert: AYE Nterm
Vector Backbone Description: Backbone Size:5371; Vector Backbone:pet29 b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12948494
Comments: A synthetic gene which placed the sequence under the control of the T7 promoter, a C-terminal 6X His tag, and codon usage optimized for Escherichia coli was obtained from BlueHeron Biotechnologies. The gene was then subcloned into the pet29 b(+) vector from Novagen.
Proper citation: RRID:Addgene_12484 Copy
Species: Homo sapiens
Genetic Insert: human APOAI gene
Vector Backbone Description: Backbone Marker:BCM; Backbone Size:24000; Vector Backbone:pD21; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12742997
Comments: Please note plasmid was difficult to sequence due to its size. Addgene NGS results contain several ambiguous bases. We advise running additional diagnostic tests before use.
The backbone was developed at BCM. The relevant reference is Proc. Natl. Acad Sci. USA 98: 13282-13287.
Proper citation: RRID:Addgene_124962 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125127 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125128 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125126 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125129 Copy
Species: Homo sapiens
Genetic Insert: SUN1_916
Vector Backbone Description: Backbone Marker:clonetech; Backbone Size:4700; Vector Backbone:ｐEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27858498
Proper citation: RRID:Addgene_125850 Copy
Species: Homo sapiens
Genetic Insert: PINK1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8688; Vector Backbone:pLenti6/V5-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15824318
Proper citation: RRID:Addgene_13318 Copy
Vector Backbone Description: Backbone Size:5891; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_120308 Copy
Genetic Insert: 2x Streptococcus pyogenes sgRNA
Vector Backbone Description: Backbone Size:2739; Vector Backbone:pTAKN2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29403014
Proper citation: RRID:Addgene_108552 Copy
Species: Mus musculus
Genetic Insert: Rlf
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pbabepuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15950903
Proper citation: RRID:Addgene_11111 Copy
Species: Homo sapiens
Genetic Insert: hTERT, p53 dominant negative
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16267004
Comments: This plasmid was generated by cloning FLAG-hTERT cDNA with flanking EcoRI-SalI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site p53DD cDNA in which HindIII/ClaI sites were again added by PCR.
Proper citation: RRID:Addgene_11128 Copy
Species: Homo sapiens
Genetic Insert: Cyclin D1, Cyclin Dependent Kinase 4
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16267004
Comments: This plasmid was generated by cloning cyclinD1 cDNA with flanking EcoRI-SalI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site CDK4R24C cDNA in which HindIII/ClaI sites were again added by PCR.
Proper citation: RRID:Addgene_11129 Copy
Species: Homo sapiens
Genetic Insert: c-myc, HRas
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16267004
Comments: Contains mouse c-myc and human Hras. This plasmid was generated by cloning c-MycT58A cDNA with flanking BamHI-EcoRI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site H-RasG12V cDNA in which HindIII/ClaI sites were again added by PCR.
Proper citation: RRID:Addgene_11130 Copy
Species: Homo sapiens
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:#21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110421 Copy
Species: Homo sapiens
Genetic Insert: Pro-SBP-mGFP-COL1A1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8102; Vector Backbone:pLVX-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30587510
Comments: See attached PDF for full descritions
Proper citation: RRID:Addgene_110726 Copy
Species: Homo sapiens
Genetic Insert: KRASG12V
Vector Backbone Description: Backbone Size:6213; Vector Backbone:mycBioID-pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30194290
Proper citation: RRID:Addgene_122211 Copy
Species: Homo sapiens
Genetic Insert: HRASG12V
Vector Backbone Description: Backbone Size:6213; Vector Backbone:mycBioID-pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30194290
Proper citation: RRID:Addgene_122214 Copy
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