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Species: Homo sapiens
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Marker:EMD Biosciences; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12218187
Proper citation: RRID:Addgene_72214 Copy
Species: Homo sapiens
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Marker:EMD Biosciences; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12218187
Proper citation: RRID:Addgene_72215 Copy
Species: Homo sapiens
Genetic Insert: Dcp2
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12218187
Comments: The depositor noted that the Dcp2 Q170R mutation found in Addgene's quality control sequence does NOT affect plasmid function.
Proper citation: RRID:Addgene_72213 Copy
Species: Mus musculus
Genetic Insert: Ten-Eleven Translocation 1
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21778364
Comments: Modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins.
Note that an additional mutation, K1581H, was introduced by PCR. This additional mutation does not affect the property of the mutant protein (as it is catalytically dead).
For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862
Proper citation: RRID:Addgene_72218 Copy
Species: Mus musculus
Genetic Insert: Ten-Eleven Translocation 2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21778364
Comments: Modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins
For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862
Proper citation: RRID:Addgene_72219 Copy
Species: Homo sapiens
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4984; Vector Backbone:pGEX-6p; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12218187
Comments: The depositor noted that the Dcp2 F146Y and E198K mutations found in Addgene's quality control sequence do NOT affect plasmid function.
Proper citation: RRID:Addgene_72216 Copy
Species: Homo sapiens
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4984; Vector Backbone:pGEX-6p; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12218187
Comments: The depositor noted that the Dcp2 F404L mutation found in Addgene's quality control sequence does NOT affect plasmid function.
Proper citation: RRID:Addgene_72217 Copy
Species: Homo sapiens
Genetic Insert: SMN2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10339583
Comments: Please note that the Addgene verified sequence detects multiple SNPs compared to the NCBI reference #NG_008728.1. The depositor confirmed that these mutations should not affect plasmid function.
Proper citation: RRID:Addgene_72287 Copy
Species: Clostridium Cellulolyticum
Genetic Insert: Clostridium Cellulolyticum Type I Dockerin
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:5648; Vector Backbone:pMAL-c5G; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27699856
Proper citation: RRID:Addgene_72200 Copy
Species: Homo sapiens
Genetic Insert: AB42
Vector Backbone Description: Backbone Marker:Waldo, et al. PMID 10404163 ; Backbone Size:6000; Vector Backbone:pET28(A+) based GFP folding reporter; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12079364
Proper citation: RRID:Addgene_72203 Copy
Species: Homo sapiens
Genetic Insert: argonaute RISC catalytic component 2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23636329
Proper citation: RRID:Addgene_72209 Copy
Species: Homo sapiens
Genetic Insert: HHEX
Vector Backbone Description: Backbone Size:9289; Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_72354 Copy
Species: Homo sapiens
Genetic Insert: ATF4
Vector Backbone Description: Backbone Size:9289; Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_72352 Copy
Species: Homo sapiens
Genetic Insert: HHEX
Vector Backbone Description: Backbone Size:9289; Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_72355 Copy
Genetic Insert: RhlR
Vector Backbone Description: Vector Backbone:E. coli BW25113; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26315440
Comments: This is an ancestor strain of CY027. The chloramphenicol resistance marker can be removed by Flp-FRT recombination to obtain CY027.
Proper citation: RRID:Addgene_72340 Copy
Vector Backbone Description: Backbone Size:5751; Vector Backbone:pHLmMBP-6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26850170
Comments: Cloning note: For cloning information, please refer to Figure S1 of the manuscript associated with this plasmid.
Mutagenesis note: Quikchange mutagenesis does not work efficiently on this family of plasmids.
Citation for pHLsec from which this plasmid is derived:
A time- and cost-efficient system for high-level protein production in mammalian cells
A. R. Aricescu, W. Lu and E. Y. Jones
Acta Cryst. D62:1243-1250 (2006)
PMID:17001101
doi:10.1107/S0907444906029799
Proper citation: RRID:Addgene_72346 Copy
Vector Backbone Description: Backbone Size:5796; Vector Backbone:pHLmMBP-8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26850170
Comments: Cloning note: For cloning information, please refer to Figure S1 of the manuscript associated with this plasmid.
Mutagenesis note: Quikchange mutagenesis does not work efficiently on this family of plasmids.
Citation for pHLsec from which this plasmid is derived:
A time- and cost-efficient system for high-level protein production in mammalian cells
A. R. Aricescu, W. Lu and E. Y. Jones
Acta Cryst. D62:1243-1250 (2006)
PMID:17001101
doi:10.1107/S0907444906029799
Proper citation: RRID:Addgene_72347 Copy
Vector Backbone Description: Backbone Size:5871; Vector Backbone:pHLmMBP-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26850170
Comments: Cloning note: For cloning information, please refer to Figure S1 of the manuscript associated with this plasmid.
Mutagenesis note: Quikchange mutagenesis does not work efficiently on this family of plasmids.
Citation for pHLsec from which this plasmid is derived:
A time- and cost-efficient system for high-level protein production in mammalian cells
A. R. Aricescu, W. Lu and E. Y. Jones
Acta Cryst. D62:1243-1250 (2006)
PMID:17001101
doi:10.1107/S0907444906029799
Proper citation: RRID:Addgene_72344 Copy
Vector Backbone Description: Backbone Size:5877; Vector Backbone:pHLmMBP-3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26850170
Comments: Cloning note: For cloning information, please refer to Figure S1 of the manuscript associated with this plasmid.
Mutagenesis note: Quikchange mutagenesis does not work efficiently on this family of plasmids.
Citation for pHLsec from which this plasmid is derived:
A time- and cost-efficient system for high-level protein production in mammalian cells
A. R. Aricescu, W. Lu and E. Y. Jones
Acta Cryst. D62:1243-1250 (2006)
PMID:17001101
doi:10.1107/S0907444906029799
Proper citation: RRID:Addgene_72345 Copy
Species: Homo sapiens
Genetic Insert: SSRP1
Vector Backbone Description: Backbone Size:9289; Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_72372 Copy
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