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http://www.addgene.org/27060

Species: synthetic gene
Genetic Insert: P#38-mcherry rfp-DAS+4
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:

Proper citation: RRID:Addgene_27060 Copy   

•   Source: Addgene

http://www.addgene.org/27058

Species: synthetic gene
Genetic Insert: P#38-mcherry rfp
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:

Proper citation: RRID:Addgene_27058 Copy   

•   Source: Addgene

http://www.addgene.org/27057

Species: synthetic gene
Genetic Insert: P#38-gfp-DAS+4
Vector Backbone Description: Backbone Size:6087; Vector Backbone:pTE-0X1-GCT6; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: pTE-mcs derivative (Addgene plasmid# 20320) with GFP-DAS+4 under the control of a strong promoter. DAS+4 sequence is AANDENYSENYADAS at end of GFP.

Proper citation: RRID:Addgene_27057 Copy   

•   Source: Addgene

http://www.addgene.org/27064

Species: synthetic gene
Genetic Insert: P#38-luxA-FLAG-luxB-DAS+4
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:

Proper citation: RRID:Addgene_27064 Copy   

•   Source: Addgene

http://www.addgene.org/27155

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Glickman Lab; Backbone Size:4152; Vector Backbone:pmsg360; Vector Types:mycobacterial ko vector; Bacterial Resistance:Hygromycin
References:
Comments: Note that Addgene's sequencing results differ from the depositor's sequence at bp# 561, 565 and 829. Depositing scientist states that these differences do not affect the function of the plasmid.

Proper citation: RRID:Addgene_27155 Copy   

•   Source: Addgene

http://www.addgene.org/27374

Species:
Genetic Insert: tetR(sB), clpXtb-K127R
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:6181; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Hygromycin
References:
Comments:

Proper citation: RRID:Addgene_27374 Copy   

•   Source: Addgene

http://www.addgene.org/31851

Species:
Genetic Insert: GFP (including ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5497; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: Plasmid can also be grown in mycobacteria.

Proper citation: RRID:Addgene_31851 Copy   

•   Source: Addgene

http://www.addgene.org/31853

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:8221; Vector Backbone:pACE; Vector Types:Bacterial Expression, Escherichia coli-Mycobacterium shuttle plasmid; Bacterial Resistance:Hygromycin
References:
Comments: Plasmid can also be grown in mycobacteria. Also see PMID 15342574 for additional reference. Please note that Addgene were unable to verify the His tag in this construct. In addition there is a 1bp mismatch in our ACEpro-F sequence with the available NCBI reference. Depositing lab believes the construct is functional.

Proper citation: RRID:Addgene_31853 Copy   

•   Source: Addgene

http://www.addgene.org/31820

Species:
Genetic Insert: GFP (lacking ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5497; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: Plasmid can also be grown in mycobacteria.

Proper citation: RRID:Addgene_31820 Copy   

•   Source: Addgene

http://www.addgene.org/32177

Species:
Genetic Insert: rpsL, FRT-hyg-FRT
Vector Backbone Description: Vector Backbone:pMN250; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: This is a suicide vector for gene deletions in mycobacteria; requires rpsL mutant (streptomycin resistant)

Proper citation: RRID:Addgene_32177 Copy   

•   Source: Addgene

http://www.addgene.org/32379

Species:
Genetic Insert: int Ms6; gfpm2+; xylEm; ttsbiA, ttsbiB
Vector Backbone Description: Vector Backbone:pMl603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin
References:
Comments: This is an improved Ms6 integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE expression as reporter genes; terminators to protect expression cassette against transcriptional interference. Please note that the conserved N->S point mutation found in the Addgene QC sequence of the Ms6 integrase gene is of no consequence and the plasmid is still fully functional for integration.

Proper citation: RRID:Addgene_32379 Copy   

•   Source: Addgene

http://www.addgene.org/32378

Species:
Genetic Insert: int Giles; gfpm2+; xylEm; ttsbiA, ttsbiB
Vector Backbone Description: Vector Backbone:pML603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin
References:
Comments: This is an improved Giles integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE as reporter genes; terminators to protect expression cassette against transcriptional interference.

Proper citation: RRID:Addgene_32378 Copy   

•   Source: Addgene

http://www.addgene.org/32361

Species:
Genetic Insert: pJAZ38 ori
Vector Backbone Description: Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: This is a low copy number (one?) plasmid that co-replicates with pAL5000-derived plasmids in mycobacteria.

Proper citation: RRID:Addgene_32361 Copy   

•   Source: Addgene

http://www.addgene.org/37278

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ghader Bashiri; Backbone Size:5087; Vector Backbone:pYUB1049; Vector Types:Bacterial Expression, mycobacteria shuttle vector; Bacterial Resistance:Hygromycin
References:
Comments:

Proper citation: RRID:Addgene_37278 Copy   

•   Source: Addgene

http://www.addgene.org/30181

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
References:
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdKatuschka2. The Katuschka tandem-dimer ORF is present at bp# 44-1522

Proper citation: RRID:Addgene_30181 Copy   

•   Source: Addgene

http://www.addgene.org/30180

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
References:
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Kaede. The Kaede ORF is present at bp# 5624-6304.

Proper citation: RRID:Addgene_30180 Copy   

•   Source: Addgene

http://www.addgene.org/30182

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
References:
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdTomato. The tdTomato ORF is present at bp# 102-1532.

Proper citation: RRID:Addgene_30182 Copy   

•   Source: Addgene

http://www.addgene.org/30173

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
References:
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance.

Proper citation: RRID:Addgene_30173 Copy   

•   Source: Addgene

http://www.addgene.org/30175

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
References:
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mOrange2. The mOrange2 ORF is present at bp# 5620-6330.

Proper citation: RRID:Addgene_30175 Copy   

•   Source: Addgene

http://www.addgene.org/30179

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
References:
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mKate2. The mKate2 ORF is present at bp# 105-803.

Proper citation: RRID:Addgene_30179 Copy   

•   Source: Addgene