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Species: Synthetic
Genetic Insert: sgRNA targeting TRY and CPC genes
Vector Backbone Description: Vector Backbone:pCambia; Vector Types:Plant Expression, CRISPR, Plant binary vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26193878
Comments: TRY & CPC sgRNA target sequence is: AATATCTCTCTATCTCCTC
ETC2 sgRNA target sequence is: GAAGTGAGTAGCATCGAAT
See Figure 1B of the associated publication for more details.
Proper citation: RRID:Addgene_71288 Copy
Vector Backbone Description: Backbone Marker:Thomas Kuhlman; Backbone Size:5702; Vector Backbone:pTKDP-cat; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26332675
Proper citation: RRID:Addgene_71321 Copy
Vector Backbone Description: Backbone Marker:Thomas Kuhlman; Backbone Size:6047; Vector Backbone:pTKDP-hph; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26332675
Proper citation: RRID:Addgene_71322 Copy
Species: Synthetic
Genetic Insert: NADPH Alanine Dehydrogenase
Vector Backbone Description: Backbone Marker:Lucigen (Middleton, WI); Backbone Size:1800; Vector Backbone:pSMART-HCKan (AF532107.1); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34600151
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2020.08.30.274290v1.full.
Proper citation: RRID:Addgene_71326 Copy
Species: Other
Genetic Insert: 3 times VenusYFP
Vector Backbone Description: Backbone Marker:ampicillin resistant variant of pDONR-P2R-P3. attP2R-ccdB-cmR-attP3 sequences of pDONR-P2R-P3 were cloned into pGEMt-easy; Vector Backbone:p2RP3-pGEMteasy; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71271 Copy
Species: Other
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:ampicillin resistant variant of pDONR-P2R-P3. attP2R-ccdB-cmR-attP3 sequences of pDONR-P2R-P3 were cloned into pGEMt-easy; Vector Backbone:p2RP3-pGEMteasy; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71270 Copy
Vector Backbone Description: Backbone Marker:Cambia; Vector Backbone:pCAMBIA1300; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:26644504
Comments: See genbank file for best annotation of features.
Proper citation: RRID:Addgene_71275 Copy
Species: Other
Genetic Insert: T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
Vector Backbone Description: Backbone Marker:ampicillin resistant variant of pDONR-P2R-P3. attP2R-ccdB-cmR-attP3 sequences of pDONR-P2R-P3 were cloned into pGEMt-easy; Vector Backbone:p2RP3-pGEMteasy; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71273 Copy
Vector Backbone Description: Vector Backbone:pSW_1; Vector Types:Gateway vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_71279 Copy
Vector Backbone Description: Backbone Size:4963; Vector Backbone:pIL253; Vector Types:Bacterial Expression, E. coli-gram positive bacteria shuttle vector; Bacterial Resistance:Erythromycin
Defining Citation: PMID:8299952
Proper citation: RRID:Addgene_71312 Copy
Vector Backbone Description: Backbone Size:4378; Vector Backbone:pGK12; Vector Types:Bacterial Expression, E. coli-gram positive bacteria shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:11526048
Comments: In plasmid preparations from E. coli, a smaller deletion derivative of pTRK669 of about 1.7 kb may be noticeable. This smaller plasmid does not affect the functionality of pTRK669 in gram positive hosts and may not appear in those backgrounds. Addgene's quality control sequence shows the expected deletion from the smaller derivative of pTRK669.
Whole plasmid sequencing from the depositor showed deletion of the NheI site. Please see the provided digest result for reference.
Proper citation: RRID:Addgene_71313 Copy
Genetic Insert: tagRFP
Vector Backbone Description: Backbone Marker:Messing lab Plasmid 50005; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26187122
Proper citation: RRID:Addgene_71277 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Thermo-Fisher; Backbone Size:3900; Vector Backbone:PCR2-1 TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin
Comments: The poly A tail included in the sequence is sufficient for purification of full length transcript using an oligo dT column. This decreases the amount of incomplete transcripts (and therefore decreases truncated Cas9 proteins).
Proper citation: RRID:Addgene_71310 Copy
Genetic Insert: tagGFP
Vector Backbone Description: Backbone Marker:Messing lab Plasmid 50005; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26187122
Proper citation: RRID:Addgene_71278 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pIRES-hrGFP II; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20627111
Proper citation: RRID:Addgene_71317 Copy
Species: Synthetic
Genetic Insert: hOTC_Cyan Nano-lantern(Ca2+)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25831507
Proper citation: RRID:Addgene_71345 Copy
Species: Synthetic
Genetic Insert: Cyan Nano-lantern(Ca2+)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25831507
Proper citation: RRID:Addgene_71344 Copy
Species: Synthetic
Genetic Insert: Orange Nano-lantern(Ca2+)_H2B
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25831507
Proper citation: RRID:Addgene_71349 Copy
Species: Synthetic
Genetic Insert: Adenosine Kinase
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247194
Proper citation: RRID:Addgene_71290 Copy
Species: Homo sapiens
Genetic Insert: Lamp2b
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25657008
Proper citation: RRID:Addgene_71294 Copy
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