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Species: Mus musculus
Genetic Insert: Unc5b
Vector Backbone Description: Backbone Marker:Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72195 Copy
Species: Synthetic
Genetic Insert: PP7-PS-2x-yeGFP
Vector Backbone Description: Backbone Size:5435; Vector Backbone:pURA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26694838
Proper citation: RRID:Addgene_72230 Copy
Species: Other
Genetic Insert: 2x-mCherry
Vector Backbone Description: Backbone Size:4821; Vector Backbone:pKAN; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26694838
Proper citation: RRID:Addgene_72236 Copy
Species: Synthetic
Genetic Insert: PP7-PS-2x-yeGFP
Vector Backbone Description: Backbone Size:5641; Vector Backbone:pURA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26694838
Proper citation: RRID:Addgene_72234 Copy
Species: Other
Genetic Insert: KlURA3
Vector Backbone Description: Vector Backbone:pFA6aKlURA3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15260239
Comments: The Resnik and Storici Labs recommend reading the following review articles for more information on this plasmid:
- Storici and Resnick Method Enzymol 2006 PMID: 16793410
- Stuckey et al., Meth. Mol. Biol. 2011 PMID: 21660695
- Stuckey and Storici Methods Enzymol 2013 PMID: 24182920
Proper citation: RRID:Addgene_72238 Copy
Species: Mus musculus
Genetic Insert: Unc5c.a
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: We identified two previously-unreported isoforms and named them "y" and "z". As such, we designated this previously-reported isoform as isoform "a".
Proper citation: RRID:Addgene_72180 Copy
Species: Mus musculus
Genetic Insert: Unc5d.y
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: Unc5d.y is missing exons 6 and 8.
Proper citation: RRID:Addgene_72184 Copy
Species: Mus musculus
Genetic Insert: Unc5d.z
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: Unc5d.z is missing exon 6.
Proper citation: RRID:Addgene_72185 Copy
Species: Mus musculus
Genetic Insert: Unc5c.z
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: Unc5c.z is missing exon 3.
Proper citation: RRID:Addgene_72182 Copy
Species: Drosophila melanogaster
Genetic Insert: Dscam1_7.27.26
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: This transcript contains altrenative exons 4.7, 6.27 and 9.26.
Proper citation: RRID:Addgene_72189 Copy
Species: Drosophila melanogaster
Genetic Insert: Dscam1_7.27.22
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: This transcript contains altrenative exons 4.7, 6.27 and 9.22.
Proper citation: RRID:Addgene_72186 Copy
Species: Mus musculus
Genetic Insert: Ten-Eleven Translocation 2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21778364
Comments: Modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins
For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862
Proper citation: RRID:Addgene_72220 Copy
Species: Acetobacter aceti 1023
Genetic Insert: alanine racemase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16843006
Proper citation: RRID:Addgene_72225 Copy
Species: Acetobacter aceti 1023
Genetic Insert: alanine racemase
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16843006
Proper citation: RRID:Addgene_72224 Copy
Species: Homo sapiens
Genetic Insert: Kif18b
Vector Backbone Description: Vector Backbone:pcDNA4TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25307933
Proper citation: RRID:Addgene_72228 Copy
Species: S. pyogenes
Genetic Insert: mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26735016
Proper citation: RRID:Addgene_72250 Copy
Species: S. pyogenes
Genetic Insert: mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26735016
Proper citation: RRID:Addgene_72251 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:8844; Vector Backbone:pCDH-CMV-MCS; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72254 Copy
Species: Other
Genetic Insert: iCre-P2A-tdTomato-T2A-Puro
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6677; Vector Backbone:pCDH-CMV-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72255 Copy
Species: Synthetic
Genetic Insert: copGFP
Vector Backbone Description: Backbone Marker:Systembio; Vector Backbone:pCDH-CMV-MCS; Vector Types:Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72253 Copy
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