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Vector Backbone Description: Vector Backbone:pX330; pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71920 Copy
Vector Backbone Description: Vector Backbone:pX330; pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71929 Copy
Vector Backbone Description: Vector Backbone:pX330; pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71927 Copy
Vector Backbone Description: Vector Backbone:pX330; pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71928 Copy
Species: Synthetic
Genetic Insert: dCas9-VP64
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71879 Copy
Vector Backbone Description: Vector Backbone:pX330; pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71918 Copy
Vector Backbone Description: Vector Backbone:pX330; pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71917 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74800 Copy
Species: Homo sapiens
Genetic Insert: IDH1
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19359588
Proper citation: RRID:Addgene_62923 Copy
Species: Homo sapiens
Genetic Insert: IDH1
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19359588
Proper citation: RRID:Addgene_62924 Copy
Species: Synthetic
Genetic Insert: CTG REPEATS
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Addgene cannot verify or guarantee the presence of all 700 CTG repeats in this array
Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
Proper citation: RRID:Addgene_63087 Copy
Species: Homo sapiens
Genetic Insert: expanded CGG repeats (99 repeats) within the 5'UTR of FMR1
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This construct allows translation of CGG repeats into 99 glycine due to a near cognate ATG codon upstream of the repeat in the 5'UTR of FMR1 (an ACG codon that initiates weakly translation).
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
Proper citation: RRID:Addgene_63089 Copy
Genetic Insert: DV2 prM-E
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29999491
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/22/286955 for bioRxiv preprint.
Proper citation: RRID:Addgene_63161 Copy
Species: Homo sapiens
Genetic Insert: STAT3
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: CAGA (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-D2-V5
Proper citation: RRID:Addgene_64611 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73169 Copy
Species: Synthetic
Genetic Insert: Clover_PUFc
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73689 Copy
Species: Synthetic
Genetic Insert: Clover_PUFa
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73688 Copy
Species: Synthetic
Genetic Insert: BFPKRAB_PUFa
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73692 Copy
Species: Synthetic
Genetic Insert: mRuby2_PUFa
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73690 Copy
Genetic Insert: sgCentromere-20xPBSc
Vector Backbone Description: Vector Backbone:pX330; pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73696 Copy
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