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Species: Geobacillus stearothermophilus
Genetic Insert: PheB
Vector Backbone Description: Backbone Size:4719; Vector Backbone:pG1AK; Vector Types:Bacterial Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27332993
Comments: Plasmid from the Geobacillus plasmid set - a modular shuttle vector toolkit for thermophile metabolic engineering
Proper citation: RRID:Addgene_71738 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:4719; Vector Backbone:pG1AK; Vector Types:Bacterial Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27332993
Comments: Plasmid from the Geobacillus plasmid set - a modular shuttle vector toolkit for thermophile metabolic engineering
Proper citation: RRID:Addgene_71737 Copy
Genetic Insert: mGFP
Vector Backbone Description: Backbone Size:4918; Vector Backbone:pAAV; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26232228
Proper citation: RRID:Addgene_71760 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30541104
Comments: Please note that the full plasmid sequence contains a few differences compared to GenBank ID: KM977569. These differences do not affect the function of the plasmid.
Proper citation: RRID:Addgene_71764 Copy
Genetic Insert: mGFP
Vector Backbone Description: Backbone Size:4941; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26232228
Proper citation: RRID:Addgene_71761 Copy
Species: Synthetic
Genetic Insert: Apollo-NADP+
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3953; Vector Backbone:pECFP N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26878383
Proper citation: RRID:Addgene_71800 Copy
Species: Lactobacillus gasseri
Genetic Insert: gusA3
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:4568; Vector Backbone:pTRK882; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
Defining Citation: PMID:21375708
Proper citation: RRID:Addgene_71803 Copy
Genetic Insert: FL.hsp70P-Dam[4-HT-intein@L127C]-Myc-PC
Vector Backbone Description: Backbone Marker:(GenBank EF362409.1); Backbone Size:8052; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27001518
Comments: The plasmid p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycPC for expression of Dam[4-HT-intein@L127C]-PC protein was made by cloning the Drosophila Polycomb open reading frame from the previously described pNDamMyc-Pc plasmid (Tolhuis et al., 2006, PMID=16628213) between the AscI and NotI sites in p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open] vector.
Proper citation: RRID:Addgene_71808 Copy
Genetic Insert: min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[open]
Vector Backbone Description: Backbone Marker:(GenBank EF362409.1); Backbone Size:8052; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27001518
Comments: The p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] plasmid vector was constructed from the following elements: Drosophila minimal hsp70 promoter (“min.hsp70P”), STOP#1 cassette flanked by two unidirectional FRT sites, open reading frame encoding Dam-Myc fusion protein, polylinker consisting of AscI, SwaI, FseI, AsiSI and NotI sites, SV40 polyadenylation signal, attB site for phiC31 integrase-mediated transgenesis, pUC backbone, Drosophila mini-white marker and loxP site. The elements were assembled in the indicated order; the last five elements were taken as a whole from pUASTattB vector (Bischof et al., 2007, PMID=17360644; GenBank EF362409.1). The p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] vector is designed for cloning and expression of FLP-inducible N-terminal Dam fusion proteins.
Proper citation: RRID:Addgene_71809 Copy
Genetic Insert: FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[closed]
Vector Backbone Description: Backbone Marker: (GenBank EF362409.1); Backbone Size:8052; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27001518
Comments: To make the construct for Dam[4-HT-intein@L127C] alone control experiments, p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[closed], an in-frame stop codon was introduced in p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open] vector immediately after the Myc epitope coding sequence.
Proper citation: RRID:Addgene_71806 Copy
Genetic Insert: FL.hsp70P-Dam[4-HT-intein@L127C]-Myc-LAM
Vector Backbone Description: Backbone Marker:(GenBank EF362409.1); Backbone Size:8052; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27001518
Comments: The plasmid p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycLAM for expression of Dam[4-HT-intein@L127C]-LAM protein was made by cloning the Drosophila Lamin Dm0 open reading frame from the previously described pDamMyc-Dm0 plasmid (Pickersgill et al., 2006, PMID=16878134) between the AscI and NotI sites in p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open] vector.
Proper citation: RRID:Addgene_71807 Copy
Species: Synthetic
Genetic Insert: Aurein1.2+36GFP-BirA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5181; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26465072
Proper citation: RRID:Addgene_71752 Copy
Species: Synthetic
Genetic Insert: (GGS)9_(pos36GFP)_6XHis_LPETG
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5181; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26465072
Proper citation: RRID:Addgene_71754 Copy
Species: Synthetic
Genetic Insert: Aurein1.2_(pos36_GFP)-LPETG-His
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5181; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26465072
Proper citation: RRID:Addgene_71755 Copy
Species: Mus musculus
Genetic Insert: Cntn5.1
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71943 Copy
Species: Mus musculus
Genetic Insert: Cntn3
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71941 Copy
Genetic Insert: T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
Vector Backbone Description: Vector Backbone:SPynCas9; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26789497
Proper citation: RRID:Addgene_71824 Copy
Vector Backbone Description: Vector Backbone:Lentiviral; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26789497
Comments: The depositor states that discrepancies found by Addgene's NGS should not affect plasmid function.
Proper citation: RRID:Addgene_71825 Copy
Species: Mus musculus
Genetic Insert: Dscaml1
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71946 Copy
Species: Mus musculus
Genetic Insert: Flrt3
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71949 Copy
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