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Species: Homo sapiens
Genetic Insert: HIPK1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23852 Copy
Species: Homo sapiens
Genetic Insert: ITK
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23881 Copy
Species: Caenorhabditis elegans
Genetic Insert: wrmScarlet11^SEC^3xMyc
Vector Backbone Description: Backbone Size:3881; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Comments: The split-wrmScarlet system was developed and published by Goudeau et al. Genetics 2021. pGLOW63 is a derivative of published TagRFP-SEC vectors (Dickinson et al. Genetics 2015). It has a 3xMyc tag in place of 3xFlag and Lox2272 sites in place of LoxP. These features allow pGLOW63 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites. pGLOW63 must also be used in a genetic background that has wrmScarlet1-10. Made by Amelie Perez (Glow Worms '21).
Proper citation: RRID:Addgene_178877 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18094039
Comments: LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. Kabeya Y et al. (EMBO J. 2000 Nov 1. 19(21):5720-8.
Proper citation: RRID:Addgene_22405 Copy
Species: Homo sapiens
Genetic Insert: OTUD4
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:0; Vector Backbone:pDEST_Tet_ON_CMV_N_FLAG_HA_PGK_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22594 Copy
Vector Backbone Description: Backbone Size:7044; Vector Backbone:pBMTB-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other
Defining Citation: PMID:16496398
Comments: Addgene's QC sequence shows a 1bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity.
Proper citation: RRID:Addgene_22823 Copy
Species: Homo sapiens
Genetic Insert: eGFPhTERT
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pBABEhygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12198499
Proper citation: RRID:Addgene_28169 Copy
Species: Homo sapiens
Genetic Insert: Lamin A
Vector Backbone Description: Backbone Size:8589; Vector Backbone:pLenti Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124521
Comments: Addgene QC NGS analysis returned BLAST results with significant sequence identity to Lamin A/C.
Proper citation: RRID:Addgene_182673 Copy
Species: Homo sapiens
Genetic Insert: c-myc
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabepuro3:hbER tam; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15367674
Comments: Please see Littlewood et al., Nucleic Acids Res. 1995 May 25;23(10):1686-90 for more information regarding the mutant hormone binding domain of the mouse estrogen receptor (hbER Tam).
Proper citation: RRID:Addgene_19128 Copy
Species: Other
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23150568
Comments: Nishimura et al., 2009, Nature Methods
Please note that this version contains a nuclear export signal. A related construct without the NES can be found at: http://www.addgene.org/64945/
Proper citation: RRID:Addgene_47328 Copy
Species: Homo sapiens
Genetic Insert: KIBRA
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22614006
Proper citation: RRID:Addgene_40887 Copy
Genetic Insert: frt-gent-frt
Vector Backbone Description: Backbone Marker:Datensko and Wanner; Vector Backbone:pR6K; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamycin
Defining Citation: PMID:21296953
Comments: frt-gent-frt cassette was cut out of pGEM-frt-gent-frt with EcoRI and ligated into EcoRI digested pR6K. Please note that the R6K ori may be in opposite orientation relative to cassette and annotation since it was not directionally cloned.
Proper citation: RRID:Addgene_45469 Copy
Species: Homo sapiens
Genetic Insert: KRAS*G12V
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23246410
Proper citation: RRID:Addgene_46746 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid 46881; Backbone Size:6733; Vector Backbone:pDL276; Vector Types:Bacterial Expression, Other, E. coli/Gram-positive Bacterial Shuttle Vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:1409970
Comments: This plasmid may contain unstable elements. Prolonged growth in E. coli selects for deletions, but not in E. faecalis. Test multiple clones upon to ensure the full plasmid is selected.
Proper citation: RRID:Addgene_46882 Copy
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pWSK29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16547065
Proper citation: RRID:Addgene_172972 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21267423
Proper citation: RRID:Addgene_174006 Copy
Species: Other
Genetic Insert: SARS-CoV-2 ORF7a protein
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009724395.1
Proper citation: RRID:Addgene_145713 Copy
Species: Other
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009724394.1
Proper citation: RRID:Addgene_145716 Copy
Species: Other
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725303.1
Proper citation: RRID:Addgene_145715 Copy
Species: Other
Genetic Insert: SARS-CoV-2 nsp6
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725302.1
Proper citation: RRID:Addgene_145721 Copy
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