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URL: http://www.wormbase.org/db/get?name=WBStrain00037585
Proper Citation: RRID:WB-STRAIN:WBStrain00037585
Description: Caenorhabditis elegans with name F52C12.2&F52C12.6(gk3019) IV/nT1 [qIs51] (IV;V). from WB.
Species: Caenorhabditis elegans
Synonyms: F52C12.2&F52C12.6(gk3019) IV/nT1 [qIs51] (IV;V).
Notes: F52C12.2, F52C12.6. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP gk3019 homozygotes (sterile, no eggs). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GCTCTATTTTTCCCCCGAAG. External right primer: TTGCATCTTGCGGTAGACAG. Internal left primer: TTCGGAGCTTCGTTGTTTCT. Internal right primer: ATCCCGTCTCAATTGTCGTC. Internal WT amplicon: 3351 bp. Deletion size: 2297 bp. Deletion left flank: AATTTTAAAATTTTAATTCCTTGTGGCAAA. Deletion right flank: CGACTCGGAAGGCGAAATGGATTTTGAGAC.|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Affected Gene: WBGene00018679(F52C12.2)|WBGene00138717(F52C12.6)
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Source: Integrated Animals
Source Database: WormBase (WB)
