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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=6484519
Proper Citation: RRID:RGD_6484519
Description: Rattus norvegicus with name SS-ROSA26em1(SB11)Mcwi from RGD.
Species: Rattus norvegicus
Notes: This strain was produced by ZFN-stimulated knockin in the rat ROSA26 locus. ZFNs targeting the sequence CCTTCCCCCTTCTTCcctcgtGATCTGCAACTGGAGTCT were injected into SS/JrHsdMcwi rat embryos along with a plasmid template incorporating the Engrailed-2 mouse splice acceptor, a loxP site, the SB11 Sleeping Beauty transposase cDNA, and SV40 polyadenylation signal to integrate the transgene by homologous recombination. The resulting animal was confirmed by sequencing both junctions to harbor the SB11 gene knocked into the rat locus and expresses SB11 transposase in every cell by immunohistochemistry. ROSA26 is a synonym for rat Thumpd3-as1 (RGD:6491660) and is used as an official symbol for rat strain nomenclature.
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
