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University of Mississippi Medical Center Mitochondrial Research Core Facility (RRID:SCR_028584)Copy Citation Copied
Proper Citation: University of Mississippi Medical Center Mitochondrial Research Core Facility (RRID:SCR_028584)
Description: Core provides specialized expertise, instrumentation, and technical support for assessment of mitochondrial function in cells, tissues, and isolated mitochondria. Provides standardized mitochondrial isolation, experimental design consultation, assay optimization, data analysis, and interpretation. Services include high-resolution respirometry using the Oroboros O2k-FluoRespirometer, simultaneous measurement of oxygen consumption and reactive oxygen species production, mitochondrial respiratory control and coupling analyses, electron transport system complex activity assays, citrate synthase activity, mitochondrial calcium handling, and assessment of mitochondrial content and oxidative stress. Studies can be performed using isolated mitochondria, permeabilized tissue fibers, tissue homogenates, and intact cells, depending on the scientific question and sample availability. Core also assists with protocol development, selection of normalization strategies, troubleshooting, training, and preparation of methods and data for grants, presentations, and publications.
Synonyms: , University of Mississippi Medical Center (UMMC) Mitochondrial Research Core, UMMC Mitochondrial Research Core
Resource Type: access service resource, service resource, core facility
Keywords: ABRF, assessment of mitochondrial function in cells, tissues, mitochondrial isolation, experimental design consultation, assay optimization, data analysis and interpretation,
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