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Proper Citation: PRIMEGENS (RRID:SCR_005474)
Description: A Web-based Tool for High-throughput Primer and Probe Design. The program has its different utilities available on its web server. A standalone version is also available. Algorithms: * SSPD - Sequence Specific Primer Design: to design primers for each of the specific sequences given by the user in the query input file against any alternate potential hybridization with any of the sequences given in the database input file. * PSPD - Probe Specific Primer Design: to design primers it selects the gene-specific fragments (probes) to design primer pairs for their PCR amplification. * FSPD Fragment Specific Primer Design: primer design algorithm used when there is a very long query sequence for which multiple primers are required for its amplification. * Check Binding Specificity * Probe Design Only: Probe design algorithm could be used to find sequence-specific probes, which doesn''t show any blast hit against database. Such probe design has been used for targeted sequencing like agilent sure-select technology with next-generation sequencing.
Abbreviations: PRIMEGENS
Synonyms: PRIMEGENS-w3, PRIMEGENS - High-throughput Primer and Probe Design, PRIMEGENSw3
Resource Type: analysis service resource, data analysis service, production service resource, service resource, software resource
Defining Citation: PMID:21415011, PMID:18579568, PMID:17951795, PMID:12424113
Keywords: primer design, high-throughput primer, probe, microarray, analysis, blast, hybridization, primer, probe design, dna methylation
Funding: DOE ; DOD ; NIH
Availability: Free for academic use, Commercial use requires license
Resource Name: PRIMEGENS
Resource ID: SCR_005474
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Source: SciCrunch Registry (RRID:SCR_005400)
Description: Interactive portal for finding and submitting biomedical resources. Resources within SciCrunch have assigned RRIDs which are used to cite resources in scientific manuscripts. SciCrunch Registry, formerly NIF Registry, provides resources catalog. Allows to add new resources. Allows edit existing resources after registration. Curators are tasked with identifying and registering resources, examining data, writing configuration files to index and display data and keeping contents current.
URL: https://rrid.site/rin/sources/SCR_005400
