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URL: http://www.addgene.org/70703
Proper Citation: RRID:Addgene_70703
Insert Name: mammalian codon-optimized Staphylococcus aureus dCas9
Organism: S. aureus
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:26524662
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Comments: For this plasmid, the sequence of the sgRNA spacer is GGGCACGGGCAGCTTGCCGG. The length of the sgRNA (not including the spacer sequence) is 84 nucleotides, where this truncated repeat/anti-repeat sgRNA is similar to that described previously by Ran et al., Nature 2015 (PubMed ID: 25830891) and conveys higher activity than the canonical full length sgRNA.
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Source: Addgene
