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URL: http://www.addgene.org/68939
Proper Citation: RRID:Addgene_68939
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:22434850
Vector Backbone Description: Backbone Size:5743; Vector Backbone:N/A; Vector Types:Staphylococcal allelic exchange vector; Bacterial Resistance:Chloramphenicol
Comments: It is prudent to check that the pIMAY is still temperature sensitive in Staph Aureus before starting any cloning. The author found out that it could lose this function while cultured in Ecoli. That's why Ecoli bearing pIMAY MUST be cultured at 37C in Chloramphenicol (10µg/ml).
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Source: Addgene
