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Plasmid Name
RRID:Addgene_45796 RRID Copied  
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RRID:Addgene_45796
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Plasmid Information

URL: http://www.addgene.org/45796

Proper Citation: RRID:Addgene_45796

Insert Name: TN-XXL

Organism: Gallus gallus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:19160515

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin

Comments: TN-XXL is composed of two fluorescent proteins: cyan fluorescent protein (CFP) as the FRET donor and cpCitrine as the FRET acceptor. These proteins are linked by the calcium-sensitive double C-terminal lobe of troponin C (TnC). After the binding of free calcium, TnC undergoes a reversible conformational change that leads to energy transfer from the donor to the acceptor fluorophore, resulting in a drop in CFP fluorescence and an increase in cpCitrine fluorescence.

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Ratings and Alerts

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Data and Source Information

Source: Addgene